Modeling Cell Survival Fraction and Other Dose-Response Relationships for Immunodeficient C.B-17 SCID Mice Exposed to 320-kV X Rays
Abstract
US homeland security concerns related to potential misuse of γ-ray-emitting radiation sources employed in radiobiological research (eg, shielded cesium-137 irradiators) led to recommendations by the National Research Council to conduct studies into possibly replacing γ-ray irradiators used in research involving small rodent and other models with X-ray instruments. A limiting factor is suitability of the X-ray photon energy spectra. The objective of our research was to demonstrate the suitability of the radiation energy spectrum of 320-kV X rays after filtration (HVL = 4 mm Cu) for in-vivo cytotoxicity studies in immunodeficient C.B-17 SCID mice. By using a previously-published Hazard Function (HF) model to characterize dose-response relationships for in vivo bone marrow and spleen cell survival fractions and also to characterize the acute lethality risk (hematopoietic syndrome mode) we demonstrate that the filtered 320-kV X-ray beam appears suitable for such studies. A key finding for C.B-17 SCID mice when compared to results previously obtained for immunocompetent C.B-17 mice is that the immunodeficient mice appear to be more radioresistant, implicating a possible role of the immune system capacity in radiosensitivity of mammals.
- Authors:
-
[1];
- Lovelace Biomedical Research Institute, Albuquerque, NM, USA
- Sandia National Laboratories, Albuquerque, NM, USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1785301
- Grant/Contract Number:
- NA0003525, SAND2019-15284 J
- Resource Type:
- Published Article
- Journal Name:
- Dose-Response
- Additional Journal Information:
- Journal Name: Dose-Response Journal Volume: 19 Journal Issue: 2; Journal ID: ISSN 1559-3258
- Publisher:
- SAGE Publications
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Scott, Bobby R., Lin, Yong, Saxton, Bryanna, Chen, Wenshu, Potter, Charles A., and Belinsky, Steven A. Modeling Cell Survival Fraction and Other Dose-Response Relationships for Immunodeficient C.B-17 SCID Mice Exposed to 320-kV X Rays. United States: N. p., 2021.
Web. doi:10.1177/15593258211019887.
Scott, Bobby R., Lin, Yong, Saxton, Bryanna, Chen, Wenshu, Potter, Charles A., & Belinsky, Steven A. Modeling Cell Survival Fraction and Other Dose-Response Relationships for Immunodeficient C.B-17 SCID Mice Exposed to 320-kV X Rays. United States. https://doi.org/10.1177/15593258211019887
Scott, Bobby R., Lin, Yong, Saxton, Bryanna, Chen, Wenshu, Potter, Charles A., and Belinsky, Steven A. Mon .
"Modeling Cell Survival Fraction and Other Dose-Response Relationships for Immunodeficient C.B-17 SCID Mice Exposed to 320-kV X Rays". United States. https://doi.org/10.1177/15593258211019887.
@article{osti_1785301,
title = {Modeling Cell Survival Fraction and Other Dose-Response Relationships for Immunodeficient C.B-17 SCID Mice Exposed to 320-kV X Rays},
author = {Scott, Bobby R. and Lin, Yong and Saxton, Bryanna and Chen, Wenshu and Potter, Charles A. and Belinsky, Steven A.},
abstractNote = {US homeland security concerns related to potential misuse of γ-ray-emitting radiation sources employed in radiobiological research (eg, shielded cesium-137 irradiators) led to recommendations by the National Research Council to conduct studies into possibly replacing γ-ray irradiators used in research involving small rodent and other models with X-ray instruments. A limiting factor is suitability of the X-ray photon energy spectra. The objective of our research was to demonstrate the suitability of the radiation energy spectrum of 320-kV X rays after filtration (HVL = 4 mm Cu) for in-vivo cytotoxicity studies in immunodeficient C.B-17 SCID mice. By using a previously-published Hazard Function (HF) model to characterize dose-response relationships for in vivo bone marrow and spleen cell survival fractions and also to characterize the acute lethality risk (hematopoietic syndrome mode) we demonstrate that the filtered 320-kV X-ray beam appears suitable for such studies. A key finding for C.B-17 SCID mice when compared to results previously obtained for immunocompetent C.B-17 mice is that the immunodeficient mice appear to be more radioresistant, implicating a possible role of the immune system capacity in radiosensitivity of mammals.},
doi = {10.1177/15593258211019887},
journal = {Dose-Response},
number = 2,
volume = 19,
place = {United States},
year = {Mon May 31 00:00:00 EDT 2021},
month = {Mon May 31 00:00:00 EDT 2021}
}
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1177/15593258211019887
https://doi.org/10.1177/15593258211019887
Other availability
Search WorldCat to find libraries that may hold this journal
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
A Comparison of in vivo Cellular Responses to CS-137 Gamma Rays and 320-KV X Rays
journal, January 2013
- Scott, B. R.; Gott, K. M.; Potter, C. A.
- Dose-Response, Vol. 11, Issue 4
Microdosimetric and Biological Effects of Photon Irradiation at Different Energies in Bone Marrow
journal, October 2015
- Belley, Matthew D.; Ashcraft, Kathleen A.; Lee, Chen-Ting
- Radiation Research, Vol. 184, Issue 4
Characterization and dosimetry of a practical X-ray alternative to self-shielded gamma irradiators
journal, January 2011
- Mehta, Kishor; Parker, Andrew
- Radiation Physics and Chemistry, Vol. 80, Issue 1
Transitioning from Gamma Rays to X Rays for Comparable Biomedical Research Irradiations: Energy Matters
journal, April 2020
- Poirier, Yannick; Belley, Matthew D.; Dewhirst, Mark W.
- Radiation Research, Vol. 193, Issue 6
Establishing a Murine Model of the Hematopoietic Syndrome of the Acute Radiation Syndrome
journal, January 2012
- Plett, P. Artur; Sampson, Carol H.; Chua, Hui Lin
- Health Physics, Vol. 103, Issue 4
Successful Migration from Radioactive Irradiators to X-ray Irradiators in One of the Largest Medical Centers in the US
journal, January 2019
- Kamen, Jacob; Hsu, Wen-Ya; Boswell, Brandon
- Health Physics, Vol. 117, Issue 5
The Effect of Age on Two Modes of Radiation Death and on Hematopoietic Cell Survival in the Mouse
journal, November 1967
- Yuhas, John M.; Storer, John B.
- Radiation Research, Vol. 32, Issue 3
Immune Reconstitution and Thymic Involution in the Acute and Delayed Hematopoietic Radiation Syndromes
journal, January 2020
- Wu, Tong; Plett, P. Artur; Chua, Hui Lin
- Health Physics, Vol. 119, Issue 5
Comparison of X-ray vs. gamma irradiation of CPDA-1 red cells
journal, November 2005
- Janatpour, K.; Denning, L.; Nelson, K.
- Vox Sanguinis, Vol. 89, Issue 4
Relation between Lineal Energy Distribution and Relative Biological Effectiveness for Photon Beams according to the Microdosimetric Kinetic Model
journal, January 2011
- Okamoto, Hiroyuki; Kanai, Tatsuaki; Kase, Yuki
- Journal of Radiation Research, Vol. 52, Issue 1
Microdosimetry: Reflections on Harald Rossi
journal, June 2002
- M. Kellerer, A.
- Radiation Protection Dosimetry, Vol. 99, Issue 1
Radiation damage and immune suppression in splenic mononuclear cell populations
journal, February 1997
- Harrington, N. P.; Chambers, K. A.; Ross, W. M.
- Clinical & Experimental Immunology, Vol. 107, Issue 2
Replacement of 137Cs Irradiators with X-ray Irradiators
journal, January 2009
- Dodd, Brian; Vetter, Richard J.
- Health Physics, Vol. 96, Issue suppl 2
Microdosimetry of 10-15 MeV bremsstrahlung x rays: Microdosimetry of 10-15 MeV bremsstrahlung
journal, May 1984
- Amols, H. I.; Zellmer, D. L.
- Medical Physics, Vol. 11, Issue 3
Irradiation Conditions Necessary for Murine Bone Marrow Ablation Utilizing an X-Ray-Based Irradiator.
journal, November 2004
- Kennedy, Patrick J.; Wang, Lei; Burke, Michael J.
- Blood, Vol. 104, Issue 11
Biological microdosimetry based on radiation cytotoxicity data
journal, August 2012
- Scott, B. R.; Hutt, J.; Lin, Y.
- Radiation Protection Dosimetry, Vol. 153, Issue 4
Effects of Low-Dose Radiation on the Immune System of Mice after Total-Body Irradiation
journal, October 2010
- Bogdándi, Enikő Noémi; Balogh, Andrea; Felgyinszki, Nikolett
- Radiation Research, Vol. 174, Issue 4
A Comparison of Cs-137 γ Rays and 320-kV X-Rays in a Mouse Bone Marrow Transplantation Model
journal, April 2020
- Gott, Katherine M.; Potter, Charles A.; Doyle-Eisele, Melanie
- Dose-Response, Vol. 18, Issue 2
Characterization of an orthovoltage biological irradiator used for radiobiological research
journal, February 2015
- Azimi, Rezvan; Alaei, Parham; Spezi, Emiliano
- Journal of Radiation Research, Vol. 56, Issue 3
Risk Estimators for Radiation-Induced Bone Marrow Syndrome Lethality in Humans
journal, September 1988
- Scott, Bobby R.; Hahn, Fletcher F.; McClellan, Roger O.
- Risk Analysis, Vol. 8, Issue 3
A Microdosimetric-Kinetic Model for the Sensitization of V79 Cells to Radiation by Incorporation of Bromodeoxyuridine
journal, May 2001
- Hawkins, Roland B.
- Radiation Research, Vol. 155, Issue 5
Stochastic Threshold Exponential (TE) Model for Hematopoietic Tissue Reconstitution Deficit after Radiation Damage
journal, January 2014
- Scott, B. R.; Potter, C. A.
- Dose-Response, Vol. 12, Issue 3