Microbiota functional activity biosensors for characterizing nutrient metabolism in vivo
Abstract
Methods for measuring gut microbiota biochemical activities in vivo are needed to characterize its functional states in health and disease. To illustrate one approach, an arabinan-containing polysaccharide was isolated from pea fiber, its structure defined, and forward genetic and proteomic analyses used to compare its effects, versus unfractionated pea fiber and sugar beet arabinan, on a human gut bacterial strain consortium in gnotobiotic mice. We produced ‘Microbiota Functional Activity Biosensors’ (MFABs) consisting of glycans covalently linked to the surface of fluorescent paramagnetic microscopic glass beads. Three MFABs, each containing a unique glycan/fluorophore combination, were simultaneously orally gavaged into gnotobiotic mice, recovered from their intestines, and analyzed to directly quantify bacterial metabolism of structurally distinct arabinans in different human diet contexts. Colocalizing pea-fiber arabinan and another polysaccharide (glucomannan) on the bead surface enhanced in vivo degradation of glucomannan. MFABs represent a potentially versatile platform for developing new prebiotics and more nutritious foods.
- Authors:
-
[1];
[1];
[2];
[2];
[2];
[1]
- Washington Univ., St. Louis, MO (United States). Edison Family Center for Genome Sciences and Systems Biology; Washington Univ., St. Louis, MO (United States). Center for Gut Microbiome and Nutrition Research
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Mondelez International, Deerfield, IL (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division; National Institutes of Health (NIH); Damon Runyon Cancer Research Foundation; USDOE
- OSTI Identifier:
- 1785173
- Alternate Identifier(s):
- OSTI ID: 1963842
- Grant/Contract Number:
- AC05-00OR22725; SC0015662; DK70977; DK078669; DRG–2303–17; F30 DK123838
- Resource Type:
- Accepted Manuscript
- Journal Name:
- eLife
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: na; Journal ID: ISSN 2050-084X
- Publisher:
- eLife Sciences Publications, Ltd.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Wesener, Darryl A., Beller, Zachary W., Peters, Samantha L., Rajabi, Amir, Dimartino, Gianluca, Giannone, Richard J., Hettich, Robert L., and Gordon, Jeffrey I. Microbiota functional activity biosensors for characterizing nutrient metabolism in vivo. United States: N. p., 2021.
Web. doi:10.7554/elife.64478.
Wesener, Darryl A., Beller, Zachary W., Peters, Samantha L., Rajabi, Amir, Dimartino, Gianluca, Giannone, Richard J., Hettich, Robert L., & Gordon, Jeffrey I. Microbiota functional activity biosensors for characterizing nutrient metabolism in vivo. United States. https://doi.org/10.7554/elife.64478
Wesener, Darryl A., Beller, Zachary W., Peters, Samantha L., Rajabi, Amir, Dimartino, Gianluca, Giannone, Richard J., Hettich, Robert L., and Gordon, Jeffrey I. Mon .
"Microbiota functional activity biosensors for characterizing nutrient metabolism in vivo". United States. https://doi.org/10.7554/elife.64478. https://www.osti.gov/servlets/purl/1785173.
@article{osti_1785173,
title = {Microbiota functional activity biosensors for characterizing nutrient metabolism in vivo},
author = {Wesener, Darryl A. and Beller, Zachary W. and Peters, Samantha L. and Rajabi, Amir and Dimartino, Gianluca and Giannone, Richard J. and Hettich, Robert L. and Gordon, Jeffrey I.},
abstractNote = {Methods for measuring gut microbiota biochemical activities in vivo are needed to characterize its functional states in health and disease. To illustrate one approach, an arabinan-containing polysaccharide was isolated from pea fiber, its structure defined, and forward genetic and proteomic analyses used to compare its effects, versus unfractionated pea fiber and sugar beet arabinan, on a human gut bacterial strain consortium in gnotobiotic mice. We produced ‘Microbiota Functional Activity Biosensors’ (MFABs) consisting of glycans covalently linked to the surface of fluorescent paramagnetic microscopic glass beads. Three MFABs, each containing a unique glycan/fluorophore combination, were simultaneously orally gavaged into gnotobiotic mice, recovered from their intestines, and analyzed to directly quantify bacterial metabolism of structurally distinct arabinans in different human diet contexts. Colocalizing pea-fiber arabinan and another polysaccharide (glucomannan) on the bead surface enhanced in vivo degradation of glucomannan. MFABs represent a potentially versatile platform for developing new prebiotics and more nutritious foods.},
doi = {10.7554/elife.64478},
journal = {eLife},
number = na,
volume = 10,
place = {United States},
year = {Mon Mar 08 00:00:00 EST 2021},
month = {Mon Mar 08 00:00:00 EST 2021}
}
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