Septins coordinate cell wall integrity and lipid metabolism in a sphingolipid-dependent process

Mela, Alexander; Momany, Michelle

doi:10.1242/jcs.258336

Title: Septins coordinate cell wall integrity and lipid metabolism in a sphingolipid-dependent process

Journal Article · 19 April 2021 · Journal of Cell Science

DOI: https://doi.org/10.1242/jcs.258336 · OSTI ID:1780429

Mela, Alexander ^[1];

^[1]

Fungal Biology Group and Plant Biology Department, University of Georgia, 2502 Miller Plant Science Building, Athens, GA 30602, USA

ABSTRACT Septins colocalize with membrane sterol-rich regions and facilitate recruitment of cell wall synthases during wall remodeling. We show that null mutants missing an Aspergillus nidulans core septin present in hexamers and octamers (ΔaspAcdc11, ΔaspBcdc3 or ΔaspCcdc12) are sensitive to multiple cell wall-disturbing agents that activate the cell wall integrity MAPK pathway. The null mutant missing the octamer-exclusive core septin (ΔaspDcdc10) showed similar sensitivity, but only to a single cell wall-disturbing agent and the null mutant missing the noncore septin (ΔaspE) showed only very mild sensitivity to a different single agent. Core septin mutants showed changes in wall polysaccharide composition and chitin synthase localization. Mutants missing any of the five septins resisted ergosterol-disrupting agents. Hexamer mutants showed increased sensitivity to sphingolipid-disrupting agents. Core septins mislocalized after treatment with sphingolipid-disrupting agents, but not after ergosterol-disrupting agents. Our data suggest that the core septins are involved in cell wall integrity signaling, that all five septins are involved in monitoring ergosterol metabolism, that the hexamer septins are required for sphingolipid metabolism and that septins require sphingolipids to coordinate the cell wall integrity response.

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Research Organization:: Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: SC0015662

OSTI ID:: 1780429

Journal Information:: Journal of Cell Science, Journal Name: Journal of Cell Science Journal Issue: 5 Vol. 135; ISSN 0021-9533

Publisher:: The Company of BiologistsCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cooperation Between the Septins and the Actomyosin Ring and Role of a Cell-Integrity Pathway During Cell Division in Fission Yeast Wu, Jian-Qiu; Ye, Yanfang; Wang, Ning Genetics, Vol. 186, Issue 3 https://doi.org/10.1534/genetics.110.119842	journal	November 2010
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Septin Organization and Functions in Budding Yeast Glomb, Oliver; Gronemeyer, Thomas Frontiers in Cell and Developmental Biology, Vol. 4 https://doi.org/10.3389/fcell.2016.00123	journal	November 2016
The Aspergillus fumigatus cell wall integrity signaling pathway: drug target, compensatory pathways, and virulence Valiante, Vito; Macheleidt, Juliane; Föge, Martin Frontiers in Microbiology, Vol. 06 https://doi.org/10.3389/fmicb.2015.00325	journal	April 2015
Aspergillus fumigatus High Osmolarity Glycerol Mitogen Activated Protein Kinases SakA and MpkC Physically Interact During Osmotic and Cell Wall Stresses Manfiolli, Adriana Oliveira; Mattos, Eliciane Cevolani; de Assis, Leandro José Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.00918	journal	May 2019
MCC/Eisosomes Regulate Cell Wall Synthesis and Stress Responses in Fungi Foderaro, Jenna; Douglas, Lois; Konopka, James Journal of Fungi, Vol. 3, Issue 4 https://doi.org/10.3390/jof3040061	journal	November 2017
A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell Clay, Lori; Caudron, Fabrice; Denoth-Lippuner, Annina eLife, Vol. 3 https://doi.org/10.7554/eLife.01883	journal	May 2014

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