Cytokinin signaling localized in phloem noncell‐autonomously regulates cambial activity during secondary growth of Populus stems

Fu, Xiaokang; Su, Huili; Liu, Shuai; Du, Xuelian; Xu, Changzheng; Luo, Keming

doi:10.1111/nph.17255

Title: Cytokinin signaling localized in phloem noncell‐autonomously regulates cambial activity during secondary growth of Populus stems

Journal Article · 07 March 2021 · New Phytologist

DOI: https://doi.org/10.1111/nph.17255 · OSTI ID:1779377

Fu, Xiaokang ^[1]; Su, Huili ^[1]; Liu, Shuai ^[1]; Du, Xuelian ^[1];

^[1];

^[2]

Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation School of Life Sciences Southwest University Chongqing 400715 China
Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation School of Life Sciences Southwest University Chongqing 400715 China, Key Laboratory of Eco‐environments of Three Gorges Reservoir Region Ministry of Education School of Life Sciences Southwest University Chongqing 400715 China

Summary The regulation of cytokinin on secondary vascular development has been uncovered by modulating cytokinin content. However, it remains unclear how cytokinin enriched in developing secondary phloem regulates cambium activity in poplar. Here, we visualized the gradient distribution of cytokinin with a peak in the secondary phloem of poplar stem via immunohistochemical imaging, and determined the role of phloem‐located cytokinin signaling during wood formation. We generated transgenic poplar harboring cytokinin oxidase/dehydrogenase ( CKX)2 , a gene encoding a cytokinin degrading enzyme, driven by the phloem‐specific CLE41b promoter, indicating that the disruption of the cytokinin gradient pattern restricts the cambial activity. The RNA interference‐based knockdown of the histidine kinase ( HK ) genes encoding cytokinin receptors specifically in secondary phloem significantly compromised the division activity of cambial cells, whereas the phloem‐specific expression of a type‐B response regulator (RR) transcription factor stimulated cambial proliferation, providing evidence for the noncell‐autonomous regulation of local cytokinin signaling on the cambial activity. Moreover, the cambium‐specific knockdown of HK s also led to restricted cambial activity, and the defects were aggravated by the reduced cytokinin accumulation. Our results showed that local cytokinin signaling in secondary phloem regulates cambial activity noncell‐autonomously, and coordinately with its local signaling in cambium.

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Sponsoring Organization:: USDOE

OSTI ID:: 1779377

Journal Information:: New Phytologist, Journal Name: New Phytologist Journal Issue: 4 Vol. 230; ISSN 0028-646X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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