A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice
Abstract
The development of a safe and effective SARS-CoV-2 vaccine is a public health priority. We designed subunit vaccine candidates using self-assembling ferritin nanoparticles displaying one of two multimerized SARS-CoV-2 spikes: full-length ectodomain (S-Fer) or a C-terminal 70 amino-acid deletion (SΔC-Fer). Ferritin is an attractive nanoparticle platform for production of vaccines, and ferritin-based vaccines have been investigated in humans in two separate clinical trials. We confirmed proper folding and antigenicity of spike on the surface of ferritin by cryo-EM and binding to conformation-specific monoclonal antibodies. After a single immunization of mice with either of the two spike ferritin particles, a lentiviral SARS-CoV-2 pseudovirus assay revealed mean neutralizing antibody titers at least 2-fold greater than those in convalescent plasma from COVID-19 patients. Additionally, a single dose of SΔC-Fer elicited significantly higher neutralizing responses as compared to immunization with the spike receptor binding domain (RBD) monomer or spike ectodomain trimer alone. After a second dose, mice immunized with SΔC-Fer exhibited higher neutralizing titers than all other groups. Taken together, these results demonstrate that multivalent presentation of SARS-CoV-2 spike on ferritin can notably enhance elicitation of neutralizing antibodies, thus constituting a viable strategy for single-dose vaccination against COVID-19.
- Authors:
-
[1];
[2];
[6];
[7]
- Stanford Univ., CA (United States). Dept. of Biochemistry & Stanford ChEM-H
- Stanford Univ., CA (United States). Dept. of Bioengineering & James H. Clark Center
- Stanford Univ., CA (United States). Dept. of Biochemistry & Stanford ChEM-H; Stanford Univ., CA (United States). Dept. of Chemistry
- Stanford Univ., CA (United States). Dept. of Pathology; Stanford Blood Center, Palo Alto, CA (United States)
- Chan Zuckerberg Biohub, San Francisco, California 94158, United States
- Stanford Univ., CA (United States). Dept. of Bioengineering & James H. Clark Center; Chan Zuckerberg Biohub, San Francisco, CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL), Division of CryoEM and Bioimaging
- Stanford Univ., CA (United States).Dept. of Biochemistry & Stanford ChEM-H; Chan Zuckerberg Biohub, San Francisco, CA (United States)
- Publication Date:
- Research Org.:
- SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1778237
- Grant/Contract Number:
- AC02-76SF00515; DRG-2301-17; S10OD02160; P01AI120943; R01AI148382; P41GM103832
- Resource Type:
- Accepted Manuscript
- Journal Name:
- ACS Central Science
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2374-7943
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Powell, Abigail E., Zhang, Kaiming, Sanyal, Mrinmoy, Tang, Shaogeng, Weidenbacher, Payton A., Li, Shanshan, Pham, Tho D., Pak, John E., Chiu, Wah, and Kim, Peter S. A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice. United States: N. p., 2021.
Web. doi:10.1021/acscentsci.0c01405.
Powell, Abigail E., Zhang, Kaiming, Sanyal, Mrinmoy, Tang, Shaogeng, Weidenbacher, Payton A., Li, Shanshan, Pham, Tho D., Pak, John E., Chiu, Wah, & Kim, Peter S. A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice. United States. https://doi.org/10.1021/acscentsci.0c01405
Powell, Abigail E., Zhang, Kaiming, Sanyal, Mrinmoy, Tang, Shaogeng, Weidenbacher, Payton A., Li, Shanshan, Pham, Tho D., Pak, John E., Chiu, Wah, and Kim, Peter S. Tue .
"A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice". United States. https://doi.org/10.1021/acscentsci.0c01405. https://www.osti.gov/servlets/purl/1778237.
@article{osti_1778237,
title = {A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice},
author = {Powell, Abigail E. and Zhang, Kaiming and Sanyal, Mrinmoy and Tang, Shaogeng and Weidenbacher, Payton A. and Li, Shanshan and Pham, Tho D. and Pak, John E. and Chiu, Wah and Kim, Peter S.},
abstractNote = {The development of a safe and effective SARS-CoV-2 vaccine is a public health priority. We designed subunit vaccine candidates using self-assembling ferritin nanoparticles displaying one of two multimerized SARS-CoV-2 spikes: full-length ectodomain (S-Fer) or a C-terminal 70 amino-acid deletion (SΔC-Fer). Ferritin is an attractive nanoparticle platform for production of vaccines, and ferritin-based vaccines have been investigated in humans in two separate clinical trials. We confirmed proper folding and antigenicity of spike on the surface of ferritin by cryo-EM and binding to conformation-specific monoclonal antibodies. After a single immunization of mice with either of the two spike ferritin particles, a lentiviral SARS-CoV-2 pseudovirus assay revealed mean neutralizing antibody titers at least 2-fold greater than those in convalescent plasma from COVID-19 patients. Additionally, a single dose of SΔC-Fer elicited significantly higher neutralizing responses as compared to immunization with the spike receptor binding domain (RBD) monomer or spike ectodomain trimer alone. After a second dose, mice immunized with SΔC-Fer exhibited higher neutralizing titers than all other groups. Taken together, these results demonstrate that multivalent presentation of SARS-CoV-2 spike on ferritin can notably enhance elicitation of neutralizing antibodies, thus constituting a viable strategy for single-dose vaccination against COVID-19.},
doi = {10.1021/acscentsci.0c01405},
journal = {ACS Central Science},
number = 1,
volume = 7,
place = {United States},
year = {Tue Jan 05 00:00:00 EST 2021},
month = {Tue Jan 05 00:00:00 EST 2021}
}
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Works referenced in this record:
Neutralizing Antibody and Soluble ACE2 Inhibition of a Replication-Competent VSV-SARS-CoV-2 and a Clinical Isolate of SARS-CoV-2
journal, September 2020
- Case, James Brett; Rothlauf, Paul W.; Chen, Rita E.
- Cell Host & Microbe, Vol. 28, Issue 3
Structural basis of receptor recognition by SARS-CoV-2
journal, March 2020
- Shang, Jian; Ye, Gang; Shi, Ke
- Nature, Vol. 581, Issue 7807
Elicitation of Potent Neutralizing Antibody Responses by Designed Protein Nanoparticle Vaccines for SARS-CoV-2
journal, November 2020
- Walls, Alexandra C.; Fiala, Brooke; Schäfer, Alexandra
- Cell, Vol. 183, Issue 5
Safety and Immunogenicity of Two RNA-Based Covid-19 Vaccine Candidates
journal, December 2020
- Walsh, Edward E.; Frenck, Robert W.; Falsey, Ann R.
- New England Journal of Medicine, Vol. 383, Issue 25
A Multibasic Cleavage Site in the Spike Protein of SARS-CoV-2 Is Essential for Infection of Human Lung Cells
journal, May 2020
- Hoffmann, Markus; Kleine-Weber, Hannah; Pöhlmann, Stefan
- Molecular Cell, Vol. 78, Issue 4
SARS-CoV-2 vaccines in development
journal, September 2020
- Krammer, Florian
- Nature, Vol. 586, Issue 7830
Crystal structure of GCN4-pIQI, a trimeric coiled coil with buried polar residues
journal, December 1998
- Eckert, Debra M.; Malashkevich, Vladimir N.; Kim, Peter S.
- Journal of Molecular Biology, Vol. 284, Issue 4
A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV
journal, April 2020
- Yuan, Meng; Wu, Nicholas C.; Zhu, Xueyong
- Science, Vol. 368, Issue 6491
SARS-CoV-2 structure and replication characterized by in situ cryo-electron tomography
journal, November 2020
- Klein, Steffen; Cortese, Mirko; Winter, Sophie L.
- Nature Communications, Vol. 11, Issue 1
Phase I/II study of COVID-19 RNA vaccine BNT162b1 in adults
journal, August 2020
- Mulligan, Mark J.; Lyke, Kirsten E.; Kitchin, Nicholas
- Nature, Vol. 586, Issue 7830
Effects of adjuvants on IgG subclasses elicited by virus-like Particles
journal, January 2012
- Visciano, Maria Luisa; Tagliamonte, Maria; Tornesello, Maria Lina
- Journal of Translational Medicine, Vol. 10, Issue 1
Vaccine Adjuvant Systems containing monophosphoryl lipid A and QS21 induce strong and persistent humoral and T cell responses against hepatitis B surface antigen in healthy adult volunteers
journal, March 2008
- Vandepapelière, Pierre; Horsmans, Yves; Moris, Philippe
- Vaccine, Vol. 26, Issue 10
A Snapshot of the Global Race for Vaccines Targeting SARS-CoV-2 and the COVID-19 Pandemic
journal, June 2020
- Funk, Colin D.; Laferrière, Craig; Ardakani, Ali
- Frontiers in Pharmacology, Vol. 11
Development of an inactivated vaccine candidate for SARS-CoV-2
journal, May 2020
- Gao, Qiang; Bao, Linlin; Mao, Haiyan
- Science, Vol. 369, Issue 6499
Design of a broadly reactive Lyme disease vaccine
journal, May 2020
- Kamp, Heather D.; Swanson, Kurt A.; Wei, Ronnie R.
- npj Vaccines, Vol. 5, Issue 1
Identification of QS-21 as an Inflammasome-activating Molecular Component of Saponin Adjuvants
journal, January 2016
- Marty-Roix, Robyn; Vladimer, Gregory I.; Pouliot, Kimberly
- Journal of Biological Chemistry, Vol. 291, Issue 3
Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus
journal, April 2012
- Tseng, Chien-Te; Sbrana, Elena; Iwata-Yoshikawa, Naoko
- PLoS ONE, Vol. 7, Issue 4
Potent binding of 2019 novel coronavirus spike protein by a SARS coronavirus-specific human monoclonal antibody
journal, January 2020
- Tian, Xiaolong; Li, Cheng; Huang, Ailing
- Emerging Microbes & Infections, Vol. 9, Issue 1
Th1-Directing Adjuvants Increase the Immunogenicity of Oligosaccharide-Protein Conjugate Vaccines Related to Streptococcus pneumoniae Type 3
journal, November 2003
- Lefeber, D. J.; Benaissa-Trouw, B.; Vliegenthart, J. F. G.
- Infection and Immunity, Vol. 71, Issue 12
Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation
journal, February 2020
- Wrapp, Daniel; Wang, Nianshuang; Corbett, Kizzmekia S.
- Science, Vol. 367, Issue 6483
Structures and distributions of SARS-CoV-2 spike proteins on intact virions
journal, August 2020
- Ke, Zunlong; Oton, Joaquin; Qu, Kun
- Nature, Vol. 588, Issue 7838
CTFFIND4: Fast and accurate defocus estimation from electron micrographs
journal, November 2015
- Rohou, Alexis; Grigorieff, Nikolaus
- Journal of Structural Biology, Vol. 192, Issue 2
Effects of Toll-Like Receptor Stimulation on Eosinophilic Infiltration in Lungs of BALB/c Mice Immunized with UV-Inactivated Severe Acute Respiratory Syndrome-Related Coronavirus Vaccine
journal, May 2014
- Iwata-Yoshikawa, N.; Uda, A.; Suzuki, T.
- Journal of Virology, Vol. 88, Issue 15
The Crystal Structure of Ferritin from Helicobacter pylori Reveals Unusual Conformational Changes for Iron Uptake
journal, July 2009
- Cho, Ki Joon; Shin, Hye Jeong; Lee, Ji-Hye
- Journal of Molecular Biology, Vol. 390, Issue 1
Strategies to overcome host immunity to adenovirus vectors in vaccine development
journal, June 2009
- Thacker, Erin E.; Timares, Laura; Matthews, Qiana L.
- Expert Review of Vaccines, Vol. 8, Issue 6
Nanoparticle vaccines
journal, January 2014
- Zhao, Liang; Seth, Arjun; Wibowo, Nani
- Vaccine, Vol. 32, Issue 3
Recent Advances in Subunit Vaccine Carriers
journal, April 2016
- Vartak, Abhishek; Sucheck, Steven
- Vaccines, Vol. 4, Issue 2
Self-assembling influenza nanoparticle vaccines elicit broadly neutralizing H1N1 antibodies
journal, May 2013
- Kanekiyo, Masaru; Wei, Chih-Jen; Yassine, Hadi M.
- Nature, Vol. 499, Issue 7456
Hemagglutinin-stem nanoparticles generate heterosubtypic influenza protection
journal, August 2015
- Yassine, Hadi M.; Boyington, Jeffrey C.; McTamney, Patrick M.
- Nature Medicine, Vol. 21, Issue 9
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy
journal, February 2017
- Zheng, Shawn Q.; Palovcak, Eugene; Armache, Jean-Paul
- Nature Methods, Vol. 14, Issue 4
Pre-existing immunity against Ad vectors: Humoral, cellular, and innate response, what's important?
journal, October 2014
- Fausther-Bovendo, Hugues; Kobinger, Gary P.
- Human Vaccines & Immunotherapeutics, Vol. 10, Issue 10
Human Monoclonal Antibody Combination against SARS Coronavirus: Synergy and Coverage of Escape Mutants
journal, July 2006
- ter Meulen, Jan; van den Brink, Edward N.; Poon, Leo L. M.
- PLoS Medicine, Vol. 3, Issue 7
Quantification and characterization of virus-like particles by size-exclusion chromatography and nanoparticle tracking analysis
journal, March 2017
- Steppert, Petra; Burgstaller, Daniel; Klausberger, Miriam
- Journal of Chromatography A, Vol. 1487
UCSF Chimera?A visualization system for exploratory research and analysis
journal, January 2004
- Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C.
- Journal of Computational Chemistry, Vol. 25, Issue 13
Structural and molecular basis for Ebola virus neutralization by protective human antibodies
journal, February 2016
- Misasi, J.; Gilman, M. S. A.; Kanekiyo, M.
- Science, Vol. 351, Issue 6279
Isolation of potent neutralizing antibodies from a survivor of the 2014 Ebola virus outbreak
journal, February 2016
- Bornholdt, Z. A.; Turner, H. L.; Murin, C. D.
- Science, Vol. 351, Issue 6277
A respiratory syncytial virus (RSV) F protein nanoparticle vaccine focuses antibody responses to a conserved neutralization domain
journal, May 2020
- Swanson, Kurt A.; Rainho-Tomko, Jennifer N.; Williams, Zachary P.
- Science Immunology, Vol. 5, Issue 47
Presenting native-like HIV-1 envelope trimers on ferritin nanoparticles improves their immunogenicity
journal, September 2015
- Sliepen, Kwinten; Ozorowski, Gabriel; Burger, Judith A.
- Retrovirology, Vol. 12, Issue 1
Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis
journal, December 2020
- Herrera, Natalia G.; Morano, Nicholas C.; Celikgil, Alev
- ACS Omega, Vol. 6, Issue 1
Subunit Vaccines Against Emerging Pathogenic Human Coronaviruses
journal, February 2020
- Wang, Ning; Shang, Jian; Jiang, Shibo
- Frontiers in Microbiology, Vol. 11
A human neutralizing antibody targets the receptor-binding site of SARS-CoV-2
journal, May 2020
- Shi, Rui; Shan, Chao; Duan, Xiaomin
- Nature, Vol. 584, Issue 7819
A novel receptor-binding domain (RBD)-based mRNA vaccine against SARS-CoV-2
journal, August 2020
- Tai, Wanbo; Zhang, Xiujuan; Drelich, Aleksandra
- Cell Research, Vol. 30, Issue 10
EMAN2: An extensible image processing suite for electron microscopy
journal, January 2007
- Tang, Guang; Peng, Liwei; Baldwin, Philip R.
- Journal of Structural Biology, Vol. 157, Issue 1
The COVID-19 vaccine development landscape
journal, April 2020
- Thanh Le, Tung; Andreadakis, Zacharias; Kumar, Arun
- Nature Reviews Drug Discovery, Vol. 19, Issue 5
Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19
journal, July 2020
- Graham, Simon P.; McLean, Rebecca K.; Spencer, Alexandra J.
- npj Vaccines, Vol. 5, Issue 1
Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays
journal, May 2020
- Crawford, Katharine H. D.; Eguia, Rachel; Dingens, Adam S.
- Viruses, Vol. 12, Issue 5
Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen
journal, August 2017
- Pallesen, Jesper; Wang, Nianshuang; Corbett, Kizzmekia S.
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 35
ReScan, a Multiplex Diagnostic Pipeline, Pans Human Sera for SARS-CoV-2 Antigens
journal, October 2020
- Zamecnik, Colin R.; Rajan, Jayant V.; Yamauchi, Kevin A.
- Cell Reports Medicine, Vol. 1, Issue 7
Characterization of the receptor-binding domain (RBD) of 2019 novel coronavirus: implication for development of RBD protein as a viral attachment inhibitor and vaccine
journal, March 2020
- Tai, Wanbo; He, Lei; Zhang, Xiujuan
- Cellular & Molecular Immunology, Vol. 17, Issue 6
RELION: Implementation of a Bayesian approach to cryo-EM structure determination
journal, December 2012
- Scheres, Sjors H. W.
- Journal of Structural Biology, Vol. 180, Issue 3, p. 519-530
Glycoproteomics ofN-glycosylation by in-gel deglycosylation and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry mapping: Application to congenital disorders of glycosylation
journal, July 2005
- Šagi, Dijana; Kienz, Petra; Denecke, Jonas
- PROTEOMICS, Vol. 5, Issue 10
Site-specific glycan analysis of the SARS-CoV-2 spike
journal, May 2020
- Watanabe, Yasunori; Allen, Joel D.; Wrapp, Daniel
- Science, Vol. 369, Issue 6501
A systematic review of SARS-CoV-2 vaccine candidates
journal, October 2020
- Dong, Yetian; Dai, Tong; Wei, Yujun
- Signal Transduction and Targeted Therapy, Vol. 5, Issue 1
Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor
journal, March 2020
- Lan, Jun; Ge, Jiwan; Yu, Jinfang
- Nature, Vol. 581, Issue 7807
Self-assembling protein nanoparticles in the design of vaccines
journal, January 2016
- López-Sagaseta, Jacinto; Malito, Enrico; Rappuoli, Rino
- Computational and Structural Biotechnology Journal, Vol. 14
Quantitative dot-blot assay for proteins using enhanced chemiluminescence
journal, August 1992
- Heinicke, E.; Kumar, U.; Munoz, D. G.
- Journal of Immunological Methods, Vol. 152, Issue 2
Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein
journal, April 2020
- Walls, Alexandra C.; Park, Young-Jun; Tortorici, M. Alejandra
- Cell, Vol. 181, Issue 2
Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model
journal, June 2020
- Rogers, Thomas F.; Zhao, Fangzhu; Huang, Deli
- Science, Vol. 369, Issue 6506
Convergent antibody responses to SARS-CoV-2 in convalescent individuals
journal, June 2020
- Robbiani, Davide F.; Gaebler, Christian; Muecksch, Frauke
- Nature, Vol. 584, Issue 7821
A neutralizing human antibody binds to the N-terminal domain of the Spike protein of SARS-CoV-2
journal, June 2020
- Chi, Xiangyang; Yan, Renhong; Zhang, Jun
- Science, Vol. 369, Issue 6504
Safety and immunogenicity of the ChAdOx1 nCoV-19 vaccine against SARS-CoV-2: a preliminary report of a phase 1/2, single-blind, randomised controlled trial
journal, August 2020
- Folegatti, Pedro M.; Ewer, Katie J.; Aley, Parvinder K.
- The Lancet, Vol. 396, Issue 10249
Potential for developing a SARS-CoV receptor-binding domain (RBD) recombinant protein as a heterologous human vaccine against coronavirus infectious disease (COVID)-19
journal, April 2020
- Chen, Wen-Hsiang; Hotez, Peter J.; Bottazzi, Maria Elena
- Human Vaccines & Immunotherapeutics, Vol. 16, Issue 6
Nanoparticle Vaccines Adopting Virus-like Features for Enhanced Immune Potentiation
journal, January 2017
- Chattopadhyay, Saborni; Chen, Jui-Yi; Chen, Hui-Wen
- Nanotheranostics, Vol. 1, Issue 3
Presenting native-like trimeric HIV-1 antigens with self-assembling nanoparticles
journal, June 2016
- He, Linling; de Val, Natalia; Morris, Charles D.
- Nature Communications, Vol. 7, Issue 1
Pre-fusion structure of a human coronavirus spike protein
journal, March 2016
- Kirchdoerfer, Robert N.; Cottrell, Christopher A.; Wang, Nianshuang
- Nature, Vol. 531, Issue 7592
A serological assay to detect SARS-CoV-2 seroconversion in humans
journal, May 2020
- Amanat, Fatima; Stadlbauer, Daniel; Strohmeier, Shirin
- Nature Medicine, Vol. 26, Issue 7
SARS-CoV-2 mRNA vaccine design enabled by prototype pathogen preparedness
journal, August 2020
- Corbett, Kizzmekia S.; Edwards, Darin K.; Leist, Sarah R.
- Nature, Vol. 586, Issue 7830
Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion
journal, March 2020
- Xia, Shuai; Liu, Meiqin; Wang, Chao
- Cell Research, Vol. 30, Issue 4
ChAdOx1 nCoV-19 vaccine prevents SARS-CoV-2 pneumonia in rhesus macaques
journal, July 2020
- van Doremalen, Neeltje; Lambe, Teresa; Spencer, Alexandra
- Nature, Vol. 586, Issue 7830
Immunogenicity of a DNA vaccine candidate for COVID-19
journal, May 2020
- Smith, Trevor R. F.; Patel, Ami; Ramos, Stephanie
- Nature Communications, Vol. 11, Issue 1
A platform incorporating trimeric antigens into self-assembling nanoparticles reveals SARS-CoV-2-spike nanoparticles to elicit substantially higher neutralizing responses than spike alone
journal, October 2020
- Zhang, Baoshan; Chao, Cara W.; Tsybovsky, Yaroslav
- Scientific Reports, Vol. 10, Issue 1
Ad26 vector-based COVID-19 vaccine encoding a prefusion-stabilized SARS-CoV-2 Spike immunogen induces potent humoral and cellular immune responses
journal, September 2020
- Bos, Rinke; Rutten, Lucy; van der Lubbe, Joan E. M.
- npj Vaccines, Vol. 5, Issue 1
Vaccine Adjuvant Systems containing monophosphoryl lipid A and QS-21 induce strong humoral and cellular immune responses against hepatitis B surface antigen which persist for at least 4 years after vaccination
journal, February 2015
- Leroux-Roels, Geert; Van Belle, Pascale; Vandepapeliere, Pierre
- Vaccine, Vol. 33, Issue 8
Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2
journal, March 2020
- Yan, Renhong; Zhang, Yuanyuan; Li, Yaning
- Science, Vol. 367, Issue 6485
Development of a mammalian cell culture process for rapid Clinical-Scale production of novel Influenza Nanoparticle vaccines
journal, December 2015
- Biswas, Payal; Trozado, Christian; Lee, James
- BMC Proceedings, Vol. 9, Issue S9
SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation
journal, December 2020
- Lederer, Katlyn; Castaño, Diana; Gómez Atria, Daniela
- Immunity, Vol. 53, Issue 6
cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination
journal, February 2017
- Punjani, Ali; Rubinstein, John L.; Fleet, David J.
- Nature Methods, Vol. 14, Issue 3
In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges
journal, August 2020
- Turoňová, Beata; Sikora, Mateusz; Schürmann, Christoph
- Science, Vol. 370, Issue 6513
Severe Acute Respiratory Syndrome-Associated Coronavirus Vaccines Formulated with Delta Inulin Adjuvants Provide Enhanced Protection while Ameliorating Lung Eosinophilic Immunopathology
journal, December 2014
- Honda-Okubo, Yoshikazu; Barnard, Dale; Ong, Chun Hao
- Journal of Virology, Vol. 89, Issue 6
Modern Subunit Vaccines: Development, Components, and Research Opportunities
journal, January 2013
- Moyle, Peter Michael; Toth, Istvan
- ChemMedChem, Vol. 8, Issue 3
Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Primates
journal, October 2020
- Corbett, Kizzmekia S.; Flynn, Barbara; Foulds, Kathryn E.
- New England Journal of Medicine, Vol. 383, Issue 16
Rational Design of an Epstein-Barr Virus Vaccine Targeting the Receptor-Binding Site
journal, August 2015
- Kanekiyo, Masaru; Bu, Wei; Joyce, M. Gordon
- Cell, Vol. 162, Issue 5
Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability
journal, June 2020
- Brouwer, Philip J. M.; Caniels, Tom G.; van der Straten, Karlijn
- Science, Vol. 369, Issue 6504
Development of an Inactivated Vaccine Candidate, BBIBP-CorV, with Potent Protection against SARS-CoV-2
journal, August 2020
- Wang, Hui; Zhang, Yuntao; Huang, Baoying
- Cell, Vol. 182, Issue 3