Crystal structures of inhibitor complexes of M‐PMV protease with visible flap loops

Wosicki, Stanislaw; Kazmierczyk, Maciej; Gilski, Miroslaw; Zabranska, Helena; Pichova, Iva; Jaskolski, Mariusz

doi:10.1002/pro.4072

Title: Crystal structures of inhibitor complexes of M‐PMV protease with visible flap loops

Journal Article · 07 April 2021 · Protein Science

DOI: https://doi.org/10.1002/pro.4072 · OSTI ID:1775002

^[1]; Kazmierczyk, Maciej ^[2];

^[3]; Zabranska, Helena ^[4]; Pichova, Iva ^[4];

^[3]

Center for Biocrystallographic Research Institute of Bioorganic Chemistry, Polish Academy of Sciences Poznan Poland
Department of Crystallography, Faculty of Chemistry A. Mickiewicz University Poznan Poland
Center for Biocrystallographic Research Institute of Bioorganic Chemistry, Polish Academy of Sciences Poznan Poland, Department of Crystallography, Faculty of Chemistry A. Mickiewicz University Poznan Poland
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences Prague Czech Republic

Abstract Mason‐Pfizer monkey virus protease (PR) was crystallized in complex with two pepstatin‐based inhibitors in P 1 space group. In both crystal structures, the extended flap loops that lock the inhibitor/substrate over the active site, are visible in the electron density either completely or with only small gaps, providing the first observation of the conformation of the flap loops in dimeric complex form of this retropepsin. The H‐bond network in the active site (with D26N mutation) differs from that reported for the P 2 ₁ crystal structures and is similar to a rarely occurring system in HIV‐1 PR.

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Sponsoring Organization:: USDOE

OSTI ID:: 1775002

Journal Information:: Protein Science, Journal Name: Protein Science Journal Issue: 6 Vol. 30; ISSN 0961-8368

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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