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Title: Reversible inhibition and reactivation of electron transfer in photosystem I

Abstract

In photosystem I (PSI) complexes at room temperature light induced electron transfer can proceed down multiple protein-cofactor branches, and the process can be an order of magnitude faster on one branch compared to the other. One factor that might contribute to this branch asymmetry is a tryptophan amino acid at position 673 on the psaB protein. This amino acid is located between two of the pigments involved in electron transfer on the psaB protein branch. The corresponding residue on the psaA protein branch is a glycine amino acid. Microsecond time-resolved step-scan FTIR difference spectroscopy at 77 K has been used to study isolated PSI complexes from wild type and a mutant where the tryptophan residue was changed to phenylalanine. Photoaccumulated FTIR difference spectra indicate changes in the protein structure upon mutation. In the mutant we also find that the electron transfer processes is inhibited following long periods of repetitive flash illumination at room temperature. This is due to double protonation of the pigment involved in electron transfer. However, we show that we can restore electron transfer functionality by incubating the light-treated mutant PSI samples in the presence of newly added (not protonated) pigment.

Authors:
 [1];  [1];  [2]; ORCiD logo [2]; ORCiD logo [1]
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  1. Georgia State Univ., Atlanta, GA (United States)
  2. Univ. of Louisville at Lafayette, LA (United States)
Publication Date:
Research Org.:
Georgia State Univ., Atlanta, GA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Contributing Org.:
Georgia State University University of Louisiana At Lafayette
OSTI Identifier:
1774547
Grant/Contract Number:  
SC0017937
Resource Type:
Accepted Manuscript
Journal Name:
Photosynthesis Research
Additional Journal Information:
Journal Volume: 145; Journal Issue: 2; Journal ID: ISSN 0166-8595
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Photosynthesis; Photosystem I; Time-resolved step-scan FTIR difference spectroscopy; Site directed mutant

Citation Formats

Agarwala, Neva, Makita, Hiroki, Luo, Lujun, Xu, Wu, and Hastings, Gary. Reversible inhibition and reactivation of electron transfer in photosystem I. United States: N. p., 2020. Web. doi:10.1007/s11120-020-00760-9.
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Agarwala, Neva, Makita, Hiroki, Luo, Lujun, Xu, Wu, & Hastings, Gary. Reversible inhibition and reactivation of electron transfer in photosystem I. United States. https://doi.org/10.1007/s11120-020-00760-9
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Agarwala, Neva, Makita, Hiroki, Luo, Lujun, Xu, Wu, and Hastings, Gary. Sat . "Reversible inhibition and reactivation of electron transfer in photosystem I". United States. https://doi.org/10.1007/s11120-020-00760-9. https://www.osti.gov/servlets/purl/1774547.
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@article{osti_1774547,
title = {Reversible inhibition and reactivation of electron transfer in photosystem I},
author = {Agarwala, Neva and Makita, Hiroki and Luo, Lujun and Xu, Wu and Hastings, Gary},
abstractNote = {In photosystem I (PSI) complexes at room temperature light induced electron transfer can proceed down multiple protein-cofactor branches, and the process can be an order of magnitude faster on one branch compared to the other. One factor that might contribute to this branch asymmetry is a tryptophan amino acid at position 673 on the psaB protein. This amino acid is located between two of the pigments involved in electron transfer on the psaB protein branch. The corresponding residue on the psaA protein branch is a glycine amino acid. Microsecond time-resolved step-scan FTIR difference spectroscopy at 77 K has been used to study isolated PSI complexes from wild type and a mutant where the tryptophan residue was changed to phenylalanine. Photoaccumulated FTIR difference spectra indicate changes in the protein structure upon mutation. In the mutant we also find that the electron transfer processes is inhibited following long periods of repetitive flash illumination at room temperature. This is due to double protonation of the pigment involved in electron transfer. However, we show that we can restore electron transfer functionality by incubating the light-treated mutant PSI samples in the presence of newly added (not protonated) pigment.},
doi = {10.1007/s11120-020-00760-9},
journal = {Photosynthesis Research},
number = 2,
volume = 145,
place = {United States},
year = {Sat May 23 00:00:00 EDT 2020},
month = {Sat May 23 00:00:00 EDT 2020}
}
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Works referenced in this record:

Mutations in algal and cyanobacterial Photosystem I that independently affect the yield of initial charge separation in the two electron transfer cofactor branches
journal, January 2018

  • Badshah, Syed Lal; Sun, Junlei; Mula, Sam
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1859, Issue 1
  • DOI: 10.1016/j.bbabio.2017.10.003

Electron transfer and arrangement of the redox cofactors in photosystem I
journal, February 1997

Assignment of a kinetic component to electron transfer between iron–sulfur clusters FX and FA/B of Photosystem I
journal, November 2006

  • Byrdin, Martin; Santabarbara, Stefano; Gu, Feifei
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1757, Issue 11
  • DOI: 10.1016/j.bbabio.2006.06.016

Electron–Phonon Coupling in Cyanobacterial Photosystem I
journal, August 2018

  • Cherepanov, Dmitry A.; Milanovsky, Georgy E.; Gopta, Oksana A.
  • The Journal of Physical Chemistry B, Vol. 122, Issue 33
  • DOI: 10.1021/acs.jpcb.8b03906

Triplet states in photosystem I of spinach chloroplasts and subchloroplast particles.
journal, October 1979

  • Frank, H. A.; McLean, M. B.; Sauer, K.
  • Proceedings of the National Academy of Sciences, Vol. 76, Issue 10
  • DOI: 10.1073/pnas.76.10.5124

Structure of photosystem I
journal, October 2001

  • Fromme, Petra; Jordan, Patrick; Krauß, Norbert
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1507, Issue 1-3
  • DOI: 10.1016/S0005-2728(01)00195-5

Evidence for a new early acceptor in Photosystem I of plants. An ESR investigation of reaction center triplet yield and of the reduced intermediary acceptors
journal, January 1983

  • Gast, P.; Swarthoff, T.; Ebskamp, F. C. R.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 722, Issue 1
  • DOI: 10.1016/0005-2728(83)90170-6

Evidence for two active branches for electron transfer in photosystem I
journal, March 2001

  • Guergova-Kuras, M.; Boudreaux, B.; Joliot, A.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 8
  • DOI: 10.1073/pnas.081078898

Modification of quinone electrochemistry by the proteins in the biological electron transfer chains: examples from photosynthetic reaction centers
journal, October 2008

  • Gunner, M. R.; Madeo, Jennifer; Zhu, Zhenyu
  • Journal of Bioenergetics and Biomembranes, Vol. 40, Issue 5
  • DOI: 10.1007/s10863-008-9179-1

Vibrational spectroscopy of photosystem I
journal, January 2015

Universality of Energy and Electron Transfer Processes in Photosystem I
journal, November 1995

  • Hastings, Gary; Hoshina, Satoshi; Webber, Andrew N.
  • Biochemistry, Vol. 34, Issue 47
  • DOI: 10.1021/bi00047a017

Observation of the Reduction and Reoxidation of the Primary Electron Acceptor in Photosystem I
journal, March 1994

  • Hastings, Gary; Kleinherenbrink, Frank A. M.; Lin, Su
  • Biochemistry, Vol. 33, Issue 11
  • DOI: 10.1021/bi00177a008

Redox Potential of Quinones in Both Electron Transfer Branches of Photosystem I
journal, September 2003

  • Ishikita, Hiroshi; Knapp, Ernst-Walter
  • Journal of Biological Chemistry, Vol. 278, Issue 52
  • DOI: 10.1074/jbc.M306434200

Electron transfer pathways in photosystem I reaction centers
journal, July 2003

In Vivo Analysis of the Electron Transfer within Photosystem I:  Are the Two Phylloquinones Involved? †
journal, August 1999

  • Joliot, Pierre; Joliot, Anne
  • Biochemistry, Vol. 38, Issue 34
  • DOI: 10.1021/bi990857c

Cyclic electron flow in C3 plants
journal, May 2006

Three-dimensional structure of cyanobacterial photosystem I at 2.5 Å resolution
journal, June 2001

  • Jordan, Patrick; Fromme, Petra; Witt, Horst Tobias
  • Nature, Vol. 411, Issue 6840
  • DOI: 10.1038/35082000

Contributions of the Protein Environment to the Midpoint Potentials of the A 1 Phylloquinones and the F X Iron−Sulfur Cluster in Photosystem I †
journal, September 2007

  • Karyagina, Irina; Pushkar, Yulia; Stehlik, Dietmar
  • Biochemistry, Vol. 46, Issue 38
  • DOI: 10.1021/bi700846z

Structural Factors That Alter the Redox Potential of Quinones in Cyanobacterial and Plant Photosystem I
journal, May 2017

Loss of Phylloquinone in Chlamydomonas Affects Plastoquinone Pool Size and Photosystem II Synthesis
journal, March 2007

  • Lefebvre-Legendre, Linnka; Rappaport, Fabrice; Finazzi, Giovanni
  • Journal of Biological Chemistry, Vol. 282, Issue 18
  • DOI: 10.1074/jbc.M610249200

Spectroscopic characterization of PS I core complexes from thermophilic Synechococcus sp : Identical reoxidation kinetics of A
journal, January 1994

Directionality of electron transfer in cyanobacterial photosystem I at 298 and 77 K
journal, May 2015

Modeling electron transfer in photosystem I
journal, June 2016

Inverted-region electron transfer as a mechanism for enhancing photosynthetic solar energy conversion efficiency
journal, August 2017

  • Makita, Hiroki; Hastings, Gary
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 35
  • DOI: 10.1073/pnas.1704855114

Photosystem I with benzoquinone analogues incorporated into the A1 binding site
journal, January 2018

Quinones in the A1 binding site in photosystem I studied using time-resolved FTIR difference spectroscopy
journal, September 2017

  • Makita, Hiroki; Rohani, Leyla; Zhao, Nan
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1858, Issue 9
  • DOI: 10.1016/j.bbabio.2017.06.006

Time-resolved visible and infrared difference spectroscopy for the study of photosystem I with different quinones incorporated into the A1 binding site
journal, March 2015

  • Makita, Hiroki; Zhao, Nan; Hastings, Gary
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1847, Issue 3
  • DOI: 10.1016/j.bbabio.2014.12.007

Structure and function of wild-type and subunit-depleted photosystem I in Synechocystis
journal, September 2018

  • Malavath, Tirupathi; Caspy, Ido; Netzer-El, Sigal Y.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1859, Issue 9
  • DOI: 10.1016/j.bbabio.2018.02.002

Structure of the plant photosystem I supercomplex at 2.6 Å resolution
journal, March 2017

Double Reduction of Plastoquinone to Plastoquinol in Photosystem 1
journal, December 2011

  • McConnell, Michael D.; Cowgill, John B.; Baker, Patricia L.
  • Biochemistry, Vol. 50, Issue 51
  • DOI: 10.1021/bi201131r

Kinetic modeling of electron transfer reactions in photosystem I complexes of various structures with substituted quinone acceptors
journal, March 2017

  • Milanovsky, Georgy E.; Petrova, Anastasia A.; Cherepanov, Dmitry A.
  • Photosynthesis Research, Vol. 133, Issue 1-3
  • DOI: 10.1007/s11120-017-0366-y

Proton and electron transfer in bacterial reaction centers
journal, May 2000

  • Okamura, M. Y.; Paddock, M. L.; Graige, M. S.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1458, Issue 1
  • DOI: 10.1016/S0005-2728(00)00065-7

5'-Monohydroxyphylloquinone is the Dominant Naphthoquinone of PSI in the Green Alga Chlamydomonas reinhardtii
journal, December 2011

  • Ozawa, S. -i.; Kosugi, M.; Kashino, Y.
  • Plant and Cell Physiology, Vol. 53, Issue 1
  • DOI: 10.1093/pcp/pcr168

Spin-Correlated Radical Pairs as Quantum Sensors of Bidirectional ET Mechanisms in Photosystem I
journal, August 2019

  • Poluektov, Oleg G.; Niklas, Jens; Utschig, Lisa M.
  • The Journal of Physical Chemistry B, Vol. 123, Issue 35
  • DOI: 10.1021/acs.jpcb.9b06636

Directionality of Electron Transfer in Type I Reaction Center Proteins: High-Frequency EPR Study of PS I with Removed Iron–Sulfur Centers
journal, April 2015

  • Poluektov, Oleg G.; Utschig, Lisa M.
  • The Journal of Physical Chemistry B, Vol. 119, Issue 43
  • DOI: 10.1021/acs.jpcb.5b04063

Structure of a green algal photosystem I in complex with a large number of light-harvesting complex I subunits
journal, March 2019

Calculated vibrational properties of semiquinones in the A1 binding site in photosystem I
journal, September 2019

  • Rohani, Leyla; Makita, Hiroki; Levitz, Andrew
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1860, Issue 9
  • DOI: 10.1016/j.bbabio.2019.07.003

Orientation of P700, the primary electron donor of Photosystem I
journal, August 1990

Mechanism of proton-coupled quinone reduction in Photosystem II
journal, December 2012

  • Saito, K.; Rutherford, A. W.; Ishikita, H.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 3
  • DOI: 10.1073/pnas.1212957110

Kinetics and Energetics of Phylloquinone Reduction in Photosystem I: Insight From Modeling of the Site Directed Mutants
journal, July 2019

Modelling electron transfer in photosystem I: limits and perspectives
journal, March 2019

  • Santabarbara, Stefano; Casazza, Anna Paola; Hastings, Gary
  • Physiologia Plantarum, Vol. 166, Issue 1
  • DOI: 10.1111/ppl.12959

Interquinone Electron Transfer in Photosystem I As Evidenced by Altering the Hydrogen Bond Strength to the Phylloquinone(s)
journal, July 2010

  • Santabarbara, Stefano; Reifschneider, Kiera; Jasaitis, Audrius
  • The Journal of Physical Chemistry B, Vol. 114, Issue 28
  • DOI: 10.1021/jp1038656

Temperature Dependence of Forward and Reverse Electron Transfer from A 1 - , the Reduced Secondary Electron Acceptor in Photosystem I †
journal, June 1998

  • Schlodder, Eberhard; Falkenberg, Klaus; Gergeleit, Martin
  • Biochemistry, Vol. 37, Issue 26
  • DOI: 10.1021/bi973182r

Forward electron transfer from phylloquinone A1 to iron-sulfur centers in spinach photosystem I
journal, August 1993

Assembly of Photosystem I: II. RUBREDOXIN IS REQUIRED FOR THE
journal, March 2002

  • Shen, Gaozhong; Antonkine, Mikhail L.; van der Est, Art
  • Journal of Biological Chemistry, Vol. 277, Issue 23
  • DOI: 10.1074/jbc.M201104200

Modeling of the P700+ Charge Recombination Kinetics with Phylloquinone and Plastoquinone-9 in the A1 Site of Photosystem I
journal, December 2002

Glotaran : A Java -Based Graphical User Interface for the R Package TIMP
journal, January 2012

  • Snellenburg, Joris J.; Laptenok, Sergey P.; Seger, Ralf
  • Journal of Statistical Software, Vol. 49, Issue 3
  • DOI: 10.18637/jss.v049.i03

Protein–cofactor interactions in bioenergetic complexes: The role of the A1A and A1B phylloquinones in Photosystem I
journal, September 2009

  • Srinivasan, Nithya; Golbeck, John H.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1787, Issue 9
  • DOI: 10.1016/j.bbabio.2009.04.010

Structure of the green algal photosystem I supercomplex with a decameric light-harvesting complex I
journal, June 2019

FTIR Difference Spectroscopy in Combination with Isotope Labeling for Identification of the Carbonyl Modes of P700 and P700+ in Photosystem I
journal, February 2004

Electron Transfer in Cyanobacterial Photosystem I
journal, July 2003

  • Xu, Wu; Chitnis, Parag; Valieva, Alfia
  • Journal of Biological Chemistry, Vol. 278, Issue 30
  • DOI: 10.1074/jbc.M302962200
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