The first sequenced Sphaerotilus natans bacteriophage– characterization and potential to control its filamentous bacterium host
Abstract
ABSTRACT Bacteriophages (phages) are ubiquitous entities present in every conceivable habitat as a result of their bacterial parasitism. Their prevalence and impact in the ecology of bacterial communities and their ability to control pathogens make their characterization essential, particularly of new phages, improving knowledge and potential application. The isolation and characterization of a new lytic phage against Sphaerotilus natans strain DSM 6575, named vB_SnaP-R1 (SnaR1), is here described. Besides being the first sequenced genome of a Sphaerotilus natans infecting phage, 99% of its 41507 bp genome lacks homology with any other sequenced phage, revealing its uniqueness and previous lack of knowledge. Moreover, SnaR1 is the first Podoviridae phage described infecting this bacterium. Sphaerotilus natans is an important filamentous bacterium due to its deleterious effect on wastewater treatment plants (WWTP) and thus, phages may play a role as novel biotechnological tools against filamentous overgrowth in WWTP. The lytic spectrum of SnaR1 was restricted to its host strain, infecting only one out of three S. natans strains and infection assays revealed its ability to reduce bacterial loads. Results suggest SnaR1 as the prototype of a new phage genus and demonstrates its potential as a non-chemical alternative to reduce S. natans DSM 6575 cells.
- Authors:
-
[1];
[1];
- CEB – Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal
- Publication Date:
- Sponsoring Org.:
- USDOE Office of Nuclear Energy (NE), Nuclear Fuel Cycle and Supply Chain
- OSTI Identifier:
- 1773544
- Grant/Contract Number:
- PTDC/AAG-TEC/3331/2014; UIDB/04469/2020
- Resource Type:
- Published Article
- Journal Name:
- FEMS Microbiology Ecology
- Additional Journal Information:
- Journal Name: FEMS Microbiology Ecology Journal Volume: 97 Journal Issue: 4; Journal ID: ISSN 0168-6496
- Publisher:
- Oxford University Press
- Country of Publication:
- Netherlands
- Language:
- English
Citation Formats
Ferreira, Rute, Amado, Rui, Padrão, Jorge, Ferreira, Vânia, Dias, Nicolina M., Melo, Luís D. R., Santos, Sílvio B., and Nicolau, Ana. The first sequenced Sphaerotilus natans bacteriophage– characterization and potential to control its filamentous bacterium host. Netherlands: N. p., 2021.
Web. doi:10.1093/femsec/fiab029.
Ferreira, Rute, Amado, Rui, Padrão, Jorge, Ferreira, Vânia, Dias, Nicolina M., Melo, Luís D. R., Santos, Sílvio B., & Nicolau, Ana. The first sequenced Sphaerotilus natans bacteriophage– characterization and potential to control its filamentous bacterium host. Netherlands. https://doi.org/10.1093/femsec/fiab029
Ferreira, Rute, Amado, Rui, Padrão, Jorge, Ferreira, Vânia, Dias, Nicolina M., Melo, Luís D. R., Santos, Sílvio B., and Nicolau, Ana. Mon .
"The first sequenced Sphaerotilus natans bacteriophage– characterization and potential to control its filamentous bacterium host". Netherlands. https://doi.org/10.1093/femsec/fiab029.
@article{osti_1773544,
title = {The first sequenced Sphaerotilus natans bacteriophage– characterization and potential to control its filamentous bacterium host},
author = {Ferreira, Rute and Amado, Rui and Padrão, Jorge and Ferreira, Vânia and Dias, Nicolina M. and Melo, Luís D. R. and Santos, Sílvio B. and Nicolau, Ana},
abstractNote = {ABSTRACT Bacteriophages (phages) are ubiquitous entities present in every conceivable habitat as a result of their bacterial parasitism. Their prevalence and impact in the ecology of bacterial communities and their ability to control pathogens make their characterization essential, particularly of new phages, improving knowledge and potential application. The isolation and characterization of a new lytic phage against Sphaerotilus natans strain DSM 6575, named vB_SnaP-R1 (SnaR1), is here described. Besides being the first sequenced genome of a Sphaerotilus natans infecting phage, 99% of its 41507 bp genome lacks homology with any other sequenced phage, revealing its uniqueness and previous lack of knowledge. Moreover, SnaR1 is the first Podoviridae phage described infecting this bacterium. Sphaerotilus natans is an important filamentous bacterium due to its deleterious effect on wastewater treatment plants (WWTP) and thus, phages may play a role as novel biotechnological tools against filamentous overgrowth in WWTP. The lytic spectrum of SnaR1 was restricted to its host strain, infecting only one out of three S. natans strains and infection assays revealed its ability to reduce bacterial loads. Results suggest SnaR1 as the prototype of a new phage genus and demonstrates its potential as a non-chemical alternative to reduce S. natans DSM 6575 cells.},
doi = {10.1093/femsec/fiab029},
journal = {FEMS Microbiology Ecology},
number = 4,
volume = 97,
place = {Netherlands},
year = {Mon Feb 15 00:00:00 EST 2021},
month = {Mon Feb 15 00:00:00 EST 2021}
}
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