Increased acid resistance of the archaeon, Metallosphaera sedula by adaptive laboratory evolution

Ai, Chenbing; McCarthy, Samuel; Eckrich, Valerie; Rudrappa, Deepak; Qiu, Guanzhou; Blum, Paul

doi:10.1007/s10295-016-1812-0

Title: Increased acid resistance of the archaeon, Metallosphaera sedula by adaptive laboratory evolution

Journal Article · 01 October 2016 · Journal of Industrial Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s10295-016-1812-0 · OSTI ID:1773518

Ai, Chenbing ^[1]; McCarthy, Samuel ^[2]; Eckrich, Valerie ^[2]; Rudrappa, Deepak ^[2]; Qiu, Guanzhou ^[3]; Blum, Paul ^[2]

grid.24434.35 0000000419370060 School of Biological Sciences University of Nebraska-Lincoln E234 Beadle Center for Genetics, 1901 Vine St. 68588-0666 Lincoln NE USA, grid.216417.7 0000000103797164 School of Minerals Processing and Bioengineering Central South University Changsha China
grid.24434.35 0000000419370060 School of Biological Sciences University of Nebraska-Lincoln E234 Beadle Center for Genetics, 1901 Vine St. 68588-0666 Lincoln NE USA
grid.216417.7 0000000103797164 School of Minerals Processing and Bioengineering Central South University Changsha China

Abstract Extremely thermoacidophilic members of the Archaea such as the lithoautotroph, Metallosphaera sedula, are among the most acid resistant forms of life and are of great relevance in bioleaching. Here, adaptive laboratory evolution was used to enhance the acid resistance of this organism while genomics and transcriptomics were used in an effort to understand the molecular basis for this trait. Unlike the parental strain, the evolved derivative, M. sedula SARC-M1, grew well at pH of 0.90. Enargite (Cu3AsS4) bioleaching conducted at pH 1.20 demonstrated SARC-M1 leached 23.78 % more copper relative to the parental strain. Genome re-sequencing identified two mutations in SARC-M1 including a nonsynonymous mutation in Msed_0408 (an amino acid permease) and a deletion in pseudogene Msed_1517. Transcriptomic studies by RNA-seq of wild type and evolved strains at various low pH values demonstrated there was enhanced expression of genes in M. sedula SARC-M1 encoding membrane complexes and enzymes that extrude protons or that catalyze proton-consuming reactions. In addition, M. sedula SARC-M1 exhibited reduced expression of genes encoding enzymes that catalyze proton-generating reactions. These unique genomic and transcriptomic features support a model for increased acid resistance arising from enhanced control over cytoplasmic pH.

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Sponsoring Organization:: USDOE

OSTI ID:: 1773518

Journal Information:: Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Journal Issue: 10 Vol. 43; ISSN 1367-5435

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Germany

Language:: English

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