Single-cell measurement of plasmid copy number and promoter activity

Shao, Bin; Rammohan, Jayan; Anderson, Daniel A.; Alperovich, Nina; Ross, David; Voigt, Christopher A.

doi:10.1038/s41467-021-21734-y

Title: Single-cell measurement of plasmid copy number and promoter activity

Journal Article · 04 March 2021 · Nature Communications

DOI: https://doi.org/10.1038/s41467-021-21734-y · OSTI ID:1773180

Shao, Bin;

;

; Alperovich, Nina;

;

Abstract Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of promoters in absolute units, the variability in their activity across a population, and their quantitative toll on cellular resources, all of which provide critical insights for cellular engineering.

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Research Organization:: Massachusetts Institute of Technology (MIT), Cambridge, MA (United States)

Sponsoring Organization:: National Institutes of Standards and Technology (NIST); USDOE

OSTI ID:: 1773180

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 12; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Title: Single-cell measurement of plasmid copy number and promoter activity

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