The Sorghum bicolor Root Exudate Sorgoleone Shapes Bacterial Communities and Delays Network Formation
Abstract
Primary and secondary metabolites exuded from roots are key drivers of root-soil microbe interactions that contribute to the structure and function of microbial communities. Studies with model plants have begun to reveal the complex interactions between root exudates and soil microbes, but little is known about the influence of specialized exudates from crop plants. The aims of this work were to understand whether sorgoleone, a unique lipophilic secondary benzoquinone exuded only from the root hairs of sorghum, influences belowground microbial community structure in the field, to assess the effect of purified sorgoleone on the cultured bacteria from field soils, and to determine whether sorgoleone inhibits nitrification under field conditions. Studies were conducted comparing wild-type sorghum and lines with genetically reduced sorgoleone exudation. In the soil near roots and rhizosphere, sorgoleone influenced microbial community structure as measured by β-diversity and network analysis. Under greenhouse conditions, the soil nitrogen content was an important factor in determining the impacts of sorgoleone. Sorgoleone delayed the formation of the bacterial and archaeal networks early in plant development and only inhibited nitrification at specific sampling times under field conditions. Sorgoleone was also shown to both inhibit and promote cultured bacterial isolate growth in laboratory tests. Thesemore »
- Authors:
-
[1];
- University of Nebraska—Lincoln, Center for Plant Science Innovation, Department of Agronomy and Horticulture, Lincoln, Nebraska, USA
- University of Nebraska—Lincoln, Center for Plant Science Innovation, Department of Biochemistry, Lincoln, Nebraska, USA
- Colorado State University, Department of Agricultural Biology, Fort Collins, Colorado, USA
- Publication Date:
- Research Org.:
- Univ. of Nebraska, Lincoln, NE (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); US Dept. of Agriculture (USDA)
- OSTI Identifier:
- 1772168
- Alternate Identifier(s):
- OSTI ID: 1851911
- Grant/Contract Number:
- SC0014395; 2020-67019-31796
- Resource Type:
- Published Article
- Journal Name:
- mSystems
- Additional Journal Information:
- Journal Name: mSystems Journal Volume: 6 Journal Issue: 2; Journal ID: ISSN 2379-5077
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Microbiology; root exudates; nitrification; microbiome; sorghum; sorgoleone; nitrogen; soil property
Citation Formats
Wang, Peng, Chai, Yen Ning, Roston, Rebecca, Dayan, Franck E., Schachtman, Daniel P., and Bulgarelli, ed., Davide. The Sorghum bicolor Root Exudate Sorgoleone Shapes Bacterial Communities and Delays Network Formation. United States: N. p., 2021.
Web. doi:10.1128/mSystems.00749-20.
Wang, Peng, Chai, Yen Ning, Roston, Rebecca, Dayan, Franck E., Schachtman, Daniel P., & Bulgarelli, ed., Davide. The Sorghum bicolor Root Exudate Sorgoleone Shapes Bacterial Communities and Delays Network Formation. United States. https://doi.org/10.1128/mSystems.00749-20
Wang, Peng, Chai, Yen Ning, Roston, Rebecca, Dayan, Franck E., Schachtman, Daniel P., and Bulgarelli, ed., Davide. Tue .
"The Sorghum bicolor Root Exudate Sorgoleone Shapes Bacterial Communities and Delays Network Formation". United States. https://doi.org/10.1128/mSystems.00749-20.
@article{osti_1772168,
title = {The Sorghum bicolor Root Exudate Sorgoleone Shapes Bacterial Communities and Delays Network Formation},
author = {Wang, Peng and Chai, Yen Ning and Roston, Rebecca and Dayan, Franck E. and Schachtman, Daniel P. and Bulgarelli, ed., Davide},
abstractNote = {Primary and secondary metabolites exuded from roots are key drivers of root-soil microbe interactions that contribute to the structure and function of microbial communities. Studies with model plants have begun to reveal the complex interactions between root exudates and soil microbes, but little is known about the influence of specialized exudates from crop plants. The aims of this work were to understand whether sorgoleone, a unique lipophilic secondary benzoquinone exuded only from the root hairs of sorghum, influences belowground microbial community structure in the field, to assess the effect of purified sorgoleone on the cultured bacteria from field soils, and to determine whether sorgoleone inhibits nitrification under field conditions. Studies were conducted comparing wild-type sorghum and lines with genetically reduced sorgoleone exudation. In the soil near roots and rhizosphere, sorgoleone influenced microbial community structure as measured by β-diversity and network analysis. Under greenhouse conditions, the soil nitrogen content was an important factor in determining the impacts of sorgoleone. Sorgoleone delayed the formation of the bacterial and archaeal networks early in plant development and only inhibited nitrification at specific sampling times under field conditions. Sorgoleone was also shown to both inhibit and promote cultured bacterial isolate growth in laboratory tests. These findings provide new insights into the role of secondary metabolites in shaping the composition and function of the sorghum root-associated bacterial microbiomes. Understanding how root exudates modify soil microbiomes may potentially unlock an important tool for enhancing crop sustainability and yield in our changing environment.},
doi = {10.1128/mSystems.00749-20},
journal = {mSystems},
number = 2,
volume = 6,
place = {United States},
year = {Tue Apr 27 00:00:00 EDT 2021},
month = {Tue Apr 27 00:00:00 EDT 2021}
}
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