Title: Structural and Kinetic Characterization of Hyperthermophilic NADH-Dependent Persulfide Reductase from Archaeoglobus fulgidus

Abstract

NADH-dependent persulfide reductase (Npsr) has been proposed to facilitate dissimilatory sulfur respiration by reducing persulfide or sulfane sulfur-containing substrates to H2S. The presence of this gene in the sulfate and thiosulfate-reducing Archaeoglobus fulgidus DSM 4304 and other hyperthermophilic Archaeoglobales appears anomalous, as A. fulgidus is unable to respire S0 and grow in the presence of elemental sulfur. To assess the role of Npsr in the sulfur metabolism of A. fulgidus DSM 4304, the Npsr from A. fulgidus was characterized. AfNpsr is specific for persulfide and polysulfide as substrates in the oxidative half-reaction, exhibiting $k_{\text{cat}}/K_{\text{m}}$ on the order of 104 M-1 s-1, which is similar to the kinetic parameters observed for hyperthermophilic CoA persulfide reductases. In contrast to the bacterial Npsr, AfNpsr exhibits low disulfide reductase activity with DTNB; however, similar to the bacterial enzymes, it does not show detectable activity with CoA-disulfide, oxidized glutathione, or cystine. The 3.1 Å X-ray structure of AfNpsr reveals access to the tightly bound catalytic CoA, and the active site Cys 42 is restricted by a flexible loop (residues 60-66) that is not seen in the bacterial homologs from Shewanella loihica PV-4 and Bacillus anthracis. Unlike the bacterial enzymes, AfNpsr exhibits NADH oxidase activity and also shows no detectable activity with NADPH. Models suggest steric and electrostatic repulsions of the NADPH 2 ${}^{\prime }$ -phosphate account for the strong preference for NADH. The presence of Npsr in the nonsulfur-reducing A. fulgidus suggests that the enzyme may offer some protection against S0 or serve in another metabolic role that has yet to be identified.

Authors:

Shabdar, Sherwin ^[1]; Anaclet, Bukuru  ^[2];

• Search DOE PAGES for author "Anaclet, Bukuru"
• Search DOE PAGES for ORCID "0000-0001-8575-0422"

Castineiras, Ana Garcia ^[1]; Desir, Neyissa ^[2]; Choe, Nicholas ^[1]; Crane, III, Edward J. ^[1]; Sazinsky, Matthew H.  ^[2];

• Search DOE PAGES for author "Sazinsky, Matthew H."
• Search DOE PAGES for ORCID "0000-0001-7550-5116"

Whitman, ed., William B.

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+ Show Author Affiliations

1. Department of Biology, Pomona College, 175 West 6th Street, Claremont, CA 91711, USA
2. Department of Chemistry, Pomona College, 645 N. College Ave., Claremont, CA, 91711, USA

Publication Date:

Tue Mar 09 00:00:00 EST 2021

Research Org.:

Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Org.:

USDOE Office of Science (SC)

OSTI Identifier:

1770252

Alternate Identifier(s):

OSTI ID: 1816197

Grant/Contract Number:  

AC02-06CH11357

Resource Type:

Published Article

Journal Name:

Archaea

Additional Journal Information:

Journal Name: Archaea Journal Volume: 2021; Journal ID: ISSN 1472-3646

Publisher:

Hindawi Publishing Corporation

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY