Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves

Zhai, Zhiyang; Liu, Hui; Shanklin, John

doi:10.3390/plants10030513

Title: Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves

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Abstract

During the transformation of wild-type (WT) Arabidopsis thaliana, a T-DNA containing OLEOSIN-GFP (OLE1-GFP) was inserted by happenstance within the GBSS1 gene, resulting in significant reduction in amylose and increase in leaf oil content in the transgenic line (OG). The synergistic effect on oil accumulation of combining gbss1 with the expression of OLE1-GFP was confirmed by transforming an independent gbss1 mutant (GABI_914G01) with OLE1-GFP. The resulting OLE1-GFP/gbss1 transgenic lines showed higher leaf oil content than the individual OLE1-GFP/WT or single gbss1 mutant lines. Further stacking of the lipogenic factors WRINKLED1, Diacylglycerol O-Acyltransferase (DGAT1), and Cys-OLEOSIN1 (an engineered sesame OLEOSIN1) in OG significantly elevated its oil content in mature leaves to 2.3% of dry weight, which is 15 times higher than that in WT Arabidopsis. Inducible expression of the same lipogenic factors was shown to be an effective strategy for triacylglycerol (TAG) accumulation without incurring growth, development, and yield penalties.

Authors:

; Liu, Hui; Shanklin, John

Publication Date:: Tue Mar 09 00:00:00 EST 2021

Research Org.:: Brookhaven National Laboratory (BNL), Upton, NY (United States); Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB); USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Biological and Environmental Research (BER). Earth and Environmental Systems Science Division

OSTI Identifier:: 1769999

Alternate Identifier(s):: OSTI ID: 1783215; OSTI ID: 1784502; OSTI ID: 1991905

Report Number(s):: BNL-221392-2021-JAAM
Journal ID: ISSN 2223-7747; PLANCD; PII: plants10030513

Grant/Contract Number:: SC0018420; SC0012704; KC0304000

Resource Type:: Published Article

Journal Name:: Plants

Additional Journal Information:: Journal Name: Plants Journal Volume: 10 Journal Issue: 3; Journal ID: ISSN 2223-7747

Publisher:: MDPI AG

Country of Publication:: Netherlands

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Triacylglycerol; Fatty acids; Oleosin; Granule; Bound; Starch; Synthase; Metabolic; Engineering

Citation Formats


                    Zhai, Zhiyang, Liu, Hui, and Shanklin, John. Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves.  Netherlands: N. p., 2021. 
Web.  doi:10.3390/plants10030513.

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                    Zhai, Zhiyang, Liu, Hui, & Shanklin, John. Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves.  Netherlands.  https://doi.org/10.3390/plants10030513

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                    Zhai, Zhiyang, Liu, Hui, and Shanklin, John. Tue .  
"Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves".  Netherlands.  https://doi.org/10.3390/plants10030513.

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@article{osti_1769999,

  title        = {Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves},

  author       = {Zhai, Zhiyang and Liu, Hui and Shanklin, John},

  abstractNote = {During the transformation of wild-type (WT) Arabidopsis thaliana, a T-DNA containing OLEOSIN-GFP (OLE1-GFP) was inserted by happenstance within the GBSS1 gene, resulting in significant reduction in amylose and increase in leaf oil content in the transgenic line (OG). The synergistic effect on oil accumulation of combining gbss1 with the expression of OLE1-GFP was confirmed by transforming an independent gbss1 mutant (GABI_914G01) with OLE1-GFP. The resulting OLE1-GFP/gbss1 transgenic lines showed higher leaf oil content than the individual OLE1-GFP/WT or single gbss1 mutant lines. Further stacking of the lipogenic factors WRINKLED1, Diacylglycerol O-Acyltransferase (DGAT1), and Cys-OLEOSIN1 (an engineered sesame OLEOSIN1) in OG significantly elevated its oil content in mature leaves to 2.3% of dry weight, which is 15 times higher than that in WT Arabidopsis. Inducible expression of the same lipogenic factors was shown to be an effective strategy for triacylglycerol (TAG) accumulation without incurring growth, development, and yield penalties.},

  doi          = {10.3390/plants10030513},

  journal      = {Plants},

  number       = 3,

  volume       = 10,

  place        = {Netherlands},

  year         = {Tue Mar 09 00:00:00 EST 2021},

  month        = {Tue Mar 09 00:00:00 EST 2021}

}

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