Functional and genetic markers of niche partitioning among enigmatic members of the human oral microbiome

Shaiber, Alon; Willis, Amy D.; Delmont, Tom O.; Roux, Simon; Chen, Lin-Xing; Schmid, Abigail C.; Yousef, Mahmoud; Watson, Andrea R.; Lolans, Karen; Esen, Özcan C.; Lee, Sonny T. M.; Downey, Nora; Morrison, Hilary G.; Dewhirst, Floyd E.; Mark Welch, Jessica L.; Eren, A. Murat

doi:10.1186/s13059-020-02195-w

Title: Functional and genetic markers of niche partitioning among enigmatic members of the human oral microbiome

Journal Article · 15 December 2020 · Genome Biology (Online)

DOI: https://doi.org/10.1186/s13059-020-02195-w · OSTI ID:1769211

Shaiber, Alon; Willis, Amy D.; Delmont, Tom O.; Roux, Simon; Chen, Lin-Xing; Schmid, Abigail C.; Yousef, Mahmoud; Watson, Andrea R.; Lolans, Karen; Esen, Özcan C.; Lee, Sonny T. M.; Downey, Nora; Morrison, Hilary G.; Dewhirst, Floyd E.; Mark Welch, Jessica L.;

Abstract Introduction Microbial residents of the human oral cavity have long been a major focus of microbiology due to their influence on host health and intriguing patterns of site specificity amidst the lack of dispersal limitation. However, the determinants of niche partitioning in this habitat are yet to be fully understood, especially among taxa that belong to recently discovered branches of microbial life. Results Here, we assemble metagenomes from tongue and dental plaque samples from multiple individuals and reconstruct 790 non-redundant genomes, 43 of which resolve to TM7, a member of the Candidate Phyla Radiation, forming six monophyletic clades that distinctly associate with either plaque or tongue. Both pangenomic and phylogenomic analyses group tongue-specific clades with other host-associated TM7 genomes. In contrast, plaque-specific TM7 group with environmental TM7 genomes. Besides offering deeper insights into the ecology, evolution, and mobilome of cryptic members of the oral microbiome, our study reveals an intriguing resemblance between dental plaque and non-host environments indicated by the TM7 evolution, suggesting that plaque may have served as a stepping stone for environmental microbes to adapt to host environments for some clades of microbes. Additionally, we report that prophages are widespread among oral-associated TM7, while absent from environmental TM7, suggesting that prophages may have played a role in adaptation of TM7 to the host environment. Conclusions Our data illuminate niche partitioning of enigmatic members of the oral cavity, including TM7, SR1, and GN02, and provide genomes for poorly characterized yet prevalent members of this biome, such as uncultivated Flavobacteriaceae.

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Research Organization:: USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1769211

Journal Information:: Genome Biology (Online), Journal Name: Genome Biology (Online) Journal Issue: 1 Vol. 21; ISSN 1474-760X

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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60 APPLIED LIFE SCIENCES
Candidate phyla radiation
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Title: Functional and genetic markers of niche partitioning among enigmatic members of the human oral microbiome

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