Pectin methylesterase selectively softens the onion epidermal wall yet reduces acid-induced creep

Wang, Xuan; Wilson, Liza; Cosgrove, Daniel J.

doi:10.1093/jxb/eraa059

Title: Pectin methylesterase selectively softens the onion epidermal wall yet reduces acid-induced creep

Journal Article · 31 January 2020 · Journal of Experimental Botany

DOI: https://doi.org/10.1093/jxb/eraa059 · OSTI ID:1767734

Wang, Xuan ^[1]; Wilson, Liza ^[1];

^[1]

Pennsylvania State Univ., University Park, PA (United States)

De-esterification of homogalacturonan (HG) is thought to stiffen pectin gels and primary cell walls by increasing calcium cross-linking between HG chains. Contrary to this idea, recent studies found that HG de-esterification correlated with reduced stiffness of living tissues, measured by surface indentation. The physical basis of such apparent wall softening is unclear, but possibly involves complex biological responses to HG modification. To assess the direct physical consequences of HG de-esterification on wall mechanics without such complications, we treated isolated onion (Allium cepa) epidermal walls with pectin methylesterase (PME) and assessed wall biomechanics with indentation and tensile tests. In nanoindentation assays, PME action softened the wall (reduced the indentation modulus). In tensile force/extension assays, PME increased plasticity, but not elasticity. These softening effects are attributed, at least in part, to increased electrostatic repulsion and swelling of the wall after PME treatment. Despite softening and swelling upon HG de-esterification, PME treatment alone failed to induce cell wall creep. Instead, acid-induced creep, mediated by endogenous α-expansin, was reduced. We conclude that HG de-esterification physically softens the onion wall, yet reduces expansin-mediated wall extensibility.

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Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF); Pennsylvania State Univ., University Park, PA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001090

OSTI ID:: 1767734

Journal Information:: Journal of Experimental Botany, Journal Name: Journal of Experimental Botany Journal Issue: 9 Vol. 71; ISSN 0022-0957

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
atomic force microscopy
bio-inspired
biofuels (including algae and biomass)
biomechanics
carbon sequestration
expansin
homogalacturonan
materials and chemistry by design
membrane
nanoindentation
onion (Allium cepa) epidermis
pectin methylesterase
plant cell wall mechanics
synthesis (self-assembly)
tensile testing
wall hydration

Title: Pectin methylesterase selectively softens the onion epidermal wall yet reduces acid-induced creep

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