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Title: A proteoliposome-based system reveals how lipids control photosynthetic light harvesting

Abstract

Integral membrane proteins are exposed to a complex and dynamic lipid environment modulated by nonbilayer lipids that can influence protein functions by lipid-protein interactions. The nonbilayer lipid monogalactosyldiacylglycerol (MGDG) is the most abundant lipid in plant photosynthetic thylakoid membranes, but its impact on the functionality of energy-converting membrane protein complexes is unknown. Here, we optimized a detergent-based reconstitution protocol to develop a proteoliposome technique that incorporates the major light-harvesting complex II (LHCII) into compositionally well-defined large unilamellar lipid bilayer vesicles to study the impact of MGDG on light harvesting by LHCII. Using steady-state fluorescence spectroscopy, CD spectroscopy, and time-correlated single-photon counting, we found that both chlorophyll fluorescence quantum yields and fluorescence lifetimes clearly indicate that the presence of MGDG in lipid bilayers switches LHCII from a light-harvesting to a more energy-quenching mode that dissipates harvested light into heat. It is hypothesized that in the in vitro system developed here, MGDG controls light harvesting of LHCII by modulating the hydrostatic lateral membrane pressure profile in the lipid bilayer sensed by LHCII-bound peripheral pigments.

Authors:
; ; ORCiD logo; ; ; ORCiD logo
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Washington State Univ., Pullman, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF); USDA
OSTI Identifier:
1760921
Alternate Identifier(s):
OSTI ID: 1605269; OSTI ID: 1735368
Grant/Contract Number:  
AC02-05CH11231; AC03-76SF000098; MCB-1616982; SC0017160
Resource Type:
Published Article
Journal Name:
Journal of Biological Chemistry
Additional Journal Information:
Journal Name: Journal of Biological Chemistry Journal Volume: 295 Journal Issue: 7; Journal ID: ISSN 0021-9258
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Tietz, Stefanie, Leuenberger, Michelle, Höhner, Ricarda, Olson, Alice H., Fleming, Graham R., and Kirchhoff, Helmut. A proteoliposome-based system reveals how lipids control photosynthetic light harvesting. United States: N. p., 2020. Web. doi:10.1074/jbc.RA119.011707.
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Tietz, Stefanie, Leuenberger, Michelle, Höhner, Ricarda, Olson, Alice H., Fleming, Graham R., & Kirchhoff, Helmut. A proteoliposome-based system reveals how lipids control photosynthetic light harvesting. United States. https://doi.org/10.1074/jbc.RA119.011707
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Tietz, Stefanie, Leuenberger, Michelle, Höhner, Ricarda, Olson, Alice H., Fleming, Graham R., and Kirchhoff, Helmut. Sat . "A proteoliposome-based system reveals how lipids control photosynthetic light harvesting". United States. https://doi.org/10.1074/jbc.RA119.011707.
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@article{osti_1760921,
title = {A proteoliposome-based system reveals how lipids control photosynthetic light harvesting},
author = {Tietz, Stefanie and Leuenberger, Michelle and Höhner, Ricarda and Olson, Alice H. and Fleming, Graham R. and Kirchhoff, Helmut},
abstractNote = {Integral membrane proteins are exposed to a complex and dynamic lipid environment modulated by nonbilayer lipids that can influence protein functions by lipid-protein interactions. The nonbilayer lipid monogalactosyldiacylglycerol (MGDG) is the most abundant lipid in plant photosynthetic thylakoid membranes, but its impact on the functionality of energy-converting membrane protein complexes is unknown. Here, we optimized a detergent-based reconstitution protocol to develop a proteoliposome technique that incorporates the major light-harvesting complex II (LHCII) into compositionally well-defined large unilamellar lipid bilayer vesicles to study the impact of MGDG on light harvesting by LHCII. Using steady-state fluorescence spectroscopy, CD spectroscopy, and time-correlated single-photon counting, we found that both chlorophyll fluorescence quantum yields and fluorescence lifetimes clearly indicate that the presence of MGDG in lipid bilayers switches LHCII from a light-harvesting to a more energy-quenching mode that dissipates harvested light into heat. It is hypothesized that in the in vitro system developed here, MGDG controls light harvesting of LHCII by modulating the hydrostatic lateral membrane pressure profile in the lipid bilayer sensed by LHCII-bound peripheral pigments.},
doi = {10.1074/jbc.RA119.011707},
journal = {Journal of Biological Chemistry},
number = 7,
volume = 295,
place = {United States},
year = {Sat Feb 01 00:00:00 EST 2020},
month = {Sat Feb 01 00:00:00 EST 2020}
}
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https://doi.org/10.1074/jbc.RA119.011707
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