Evaluation of large volume yeast interfering RNA lure-and-kill ovitraps for attraction and control of Aedes mosquitoes
Abstract
Aedes mosquitoes (Diptera: Culicidae), principle vectors of several arboviruses, typically lay eggs in man-made water-filled containers located near human dwellings. Given the widespread emergence of insecticide resistance, stable and biofriendly alternatives for mosquito larviciding are needed. Laboratory studies have demonstrated that inactivated yeast interfering RNA tablets targeting key larval developmental genes can be used to facilitate effective larvicidal activity while also promoting selective gravid female oviposition behaviour. In this work, we examined the efficacy of transferring this technology toward development of lure-and-kill ovitraps targeting Aedes aegypti (L.) and Aedes albopictus (Skuse) female mosquitoes. Insectary, simulated field and semi-field experiments demonstrated that two mosquito-specific yeast interfering RNA pesticides induce high levels of mortality among larvae of both species in treated large volume containers. Small-scale field trials conducted in Trinidad, West Indies demonstrated that large volume ovitrap containers baited with inactivated yeast tablets lure significantly more gravid females than traps containing only water and were highly attractive to both A. aegypti and A. albopictus females. Finally, these studies indicate that development of biorational yeast interfering RNA-baited ovitraps may represent a new tool for control of Aedes mosquitoes, including deployment in existing lure-and-kill ovitrap technologies or traditional container larviciding programs.
- Authors:
-
[4];
- Indiana Univ. School of Medicine, South Bend, IN (United States); Univ. of Notre Dame, IN (United States)
- Univ. of the West Indies, St. Augustine (Trinidad and Tobago)
- Univ. of Notre Dame, IN (United States)
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Indiana Univ. School of Medicine, South Bend, IN (United States); Univ. of Notre Dame, IN (United States); Univ. of the West Indies, St. Augustine (Trinidad and Tobago)
- Publication Date:
- Research Org.:
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE; National Institutes of Health (NIH); USDOD
- OSTI Identifier:
- 1756832
- Report Number(s):
- LA-UR-20-29552
Journal ID: ISSN 0269-283X
- Grant/Contract Number:
- 89233218CNA000001; R21 AI128116-01; W81XWH-17-1-0294; W81XWH-17-1-0295
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Medical and Veterinary Entomology
- Additional Journal Information:
- Journal Volume: 35; Journal Issue: 3; Journal ID: ISSN 0269-283X
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biological science; Aedes aegypti; Aedes albopictus; arbovirus; behaviour; larvicide; oviposition; Trinidad West Indies
Citation Formats
Hapairi, Limb, Mysore, Keshava, James, Lester, Scheel, Nicholas, Realey, Jacob, Sun, Longhua, Gerber, Laura, Shui Feng, Rachel, Romero-Severson, Ethan, Mohammed, Azad, Duman-Scheel, Molly, and Severson, David. Evaluation of large volume yeast interfering RNA lure-and-kill ovitraps for attraction and control of Aedes mosquitoes. United States: N. p., 2020.
Web. doi:10.1111/mve.12504.
Hapairi, Limb, Mysore, Keshava, James, Lester, Scheel, Nicholas, Realey, Jacob, Sun, Longhua, Gerber, Laura, Shui Feng, Rachel, Romero-Severson, Ethan, Mohammed, Azad, Duman-Scheel, Molly, & Severson, David. Evaluation of large volume yeast interfering RNA lure-and-kill ovitraps for attraction and control of Aedes mosquitoes. United States. https://doi.org/10.1111/mve.12504
Hapairi, Limb, Mysore, Keshava, James, Lester, Scheel, Nicholas, Realey, Jacob, Sun, Longhua, Gerber, Laura, Shui Feng, Rachel, Romero-Severson, Ethan, Mohammed, Azad, Duman-Scheel, Molly, and Severson, David. Wed .
"Evaluation of large volume yeast interfering RNA lure-and-kill ovitraps for attraction and control of Aedes mosquitoes". United States. https://doi.org/10.1111/mve.12504. https://www.osti.gov/servlets/purl/1756832.
@article{osti_1756832,
title = {Evaluation of large volume yeast interfering RNA lure-and-kill ovitraps for attraction and control of Aedes mosquitoes},
author = {Hapairi, Limb and Mysore, Keshava and James, Lester and Scheel, Nicholas and Realey, Jacob and Sun, Longhua and Gerber, Laura and Shui Feng, Rachel and Romero-Severson, Ethan and Mohammed, Azad and Duman-Scheel, Molly and Severson, David},
abstractNote = {Aedes mosquitoes (Diptera: Culicidae), principle vectors of several arboviruses, typically lay eggs in man-made water-filled containers located near human dwellings. Given the widespread emergence of insecticide resistance, stable and biofriendly alternatives for mosquito larviciding are needed. Laboratory studies have demonstrated that inactivated yeast interfering RNA tablets targeting key larval developmental genes can be used to facilitate effective larvicidal activity while also promoting selective gravid female oviposition behaviour. In this work, we examined the efficacy of transferring this technology toward development of lure-and-kill ovitraps targeting Aedes aegypti (L.) and Aedes albopictus (Skuse) female mosquitoes. Insectary, simulated field and semi-field experiments demonstrated that two mosquito-specific yeast interfering RNA pesticides induce high levels of mortality among larvae of both species in treated large volume containers. Small-scale field trials conducted in Trinidad, West Indies demonstrated that large volume ovitrap containers baited with inactivated yeast tablets lure significantly more gravid females than traps containing only water and were highly attractive to both A. aegypti and A. albopictus females. Finally, these studies indicate that development of biorational yeast interfering RNA-baited ovitraps may represent a new tool for control of Aedes mosquitoes, including deployment in existing lure-and-kill ovitrap technologies or traditional container larviciding programs.},
doi = {10.1111/mve.12504},
journal = {Medical and Veterinary Entomology},
number = 3,
volume = 35,
place = {United States},
year = {2020},
month = {12}
}
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Works referenced in this record:
Sustained, Area-Wide Control of Aedes aegypti Using CDC Autocidal Gravid Ovitraps
journal, December 2014
- Barrera, Roberto; Amador, Manuel; Acevedo, Verónica
- The American Journal of Tropical Medicine and Hygiene, Vol. 91, Issue 6
A Biodegradable Lethal Ovitrap for Control of Container-Breeding Aedes
journal, March 2008
- Ritchie, Scott A.; Long, Sharron A.; McCaffrey, Nick
- Journal of the American Mosquito Control Association, Vol. 24, Issue 1
Oviposition behaviour and parity rates of Aedes aegypti collected in sticky traps in Trinidad, West Indies
journal, December 2010
- Chadee, Dave D.; Ritchie, Scott A.
- Acta Tropica, Vol. 116, Issue 3
Lure-and-Kill Yeast Interfering RNA Larvicides Targeting Neural Genes in the Human Disease Vector Mosquito Aedes aegypti
journal, October 2017
- Hapairai, Limb K.; Mysore, Keshava; Chen, Yingying
- Scientific Reports, Vol. 7, Issue 1
Contemporary status of insecticide resistance in the major Aedes vectors of arboviruses infecting humans
journal, July 2017
- Moyes, Catherine L.; Vontas, John; Martins, Ademir J.
- PLOS Neglected Tropical Diseases, Vol. 11, Issue 7
Evaluation of Alternative Killing Agents for Aedes aegypti (Diptera: Culicidae) in the Gravid Aedes Trap (GAT)
journal, May 2016
- Heringer, Laila; Johnson, Brian J.; Fikrig, Kara
- Journal of Medical Entomology, Vol. 53, Issue 4
Development of the Gravid Aedes Trap for the Capture of Adult Female Container-Exploiting Mosquitoes (Diptera: Culicidae)
journal, January 2014
- Eiras, Alvaro E.; Buhagiar, Tamara S.; Ritchie, Scott A.
- Journal of Medical Entomology, Vol. 51, Issue 1
Assembly and function of RNA silencing complexes
journal, January 2005
- Sontheimer, Erik J.
- Nature Reviews Molecular Cell Biology, Vol. 6, Issue 2
Ecotoxicological effects of larvicide used in the control of Aedes aegypti on nontarget organisms: Redefining the use of pyriproxyfen
journal, January 2017
- Vieira Santos, Vanessa Santana; Caixeta, Evelyn Siqueira; Campos Júnior, Edimar Olegário de
- Journal of Toxicology and Environmental Health, Part A, Vol. 80, Issue 3
Efficacy of Spinosad in Control of Larval Culex tarsalis and Chironomid Midges, and Its Nontarget Effects
journal, December 2013
- Lawler, Sharon P.; Dritz, Deborah A.
- Journal of the American Mosquito Control Association, Vol. 29, Issue 4
Characterization of a broad-based mosquito yeast interfering RNA larvicide with a conserved target site in mosquito semaphorin-1a genes
journal, May 2019
- Mysore, Keshava; Li, Ping; Wang, Chien-Wei
- Parasites & Vectors, Vol. 12, Issue 1
Climate, mosquito indices and the epidemiology of dengue fever in Trinidad (2002–2004)
journal, January 2007
- Chadee, D. D.; Shivnauth, B.; Rawlins, S. C.
- Annals of Tropical Medicine & Parasitology, Vol. 101, Issue 1
Ecotoxicity and Environmental Risk Assessment of Larvicides Used in the Control of Aedes aegypti to Daphnia magna (Crustacea, Cladocera)
journal, February 2014
- Abe, Flavia Renata; Coleone, Ana Carla; Machado, Angela Aparecida
- Journal of Toxicology and Environmental Health, Part A, Vol. 77, Issue 1-3
Field Evaluation of a Lethal Ovitrap for the Control of Aedes aegypti (Diptera: Culicidae) in Thailand
journal, July 2003
- Sithiprasasna, Ratana; Mahapibul, Pradith; Noigamol, Chumnong
- Journal of Medical Entomology, Vol. 40, Issue 4
Saccharomyces cerevisiae (Baker’s Yeast) as an Interfering RNA Expression and Delivery System
journal, June 2019
- Duman-Scheel, Molly
- Current Drug Targets, Vol. 20, Issue 9
Environmental safety review of methoprene and bacterially-derived pesticides commonly used for sustained mosquito control
journal, May 2017
- Lawler, Sharon P.
- Ecotoxicology and Environmental Safety, Vol. 139
Arbovirus–mosquito interactions: RNAi pathway
journal, December 2015
- Olson, Ken E.; Blair, Carol D.
- Current Opinion in Virology, Vol. 15
RNA Interference for Mosquito and Mosquito-Borne Disease Control
journal, January 2017
- Airs, Paul; Bartholomay, Lyric
- Insects, Vol. 8, Issue 1
The State of the Art of Lethal Oviposition Trap-Based Mass Interventions for Arboviral Control
journal, January 2017
- Johnson, Brian; Ritchie, Scott; Fonseca, Dina
- Insects, Vol. 8, Issue 1
An improved autocidal gravid ovitrap for the control and surveillance of Aedes aegypti
journal, August 2013
- Mackay, Andrew J.; Amador, Manuel; Barrera, Roberto
- Parasites & Vectors, Vol. 6, Issue 1
Characterization of a yeast interfering RNA larvicide with a target site conserved in the synaptotagmin gene of multiple disease vector mosquitoes
journal, May 2019
- Mysore, Keshava; Li, Ping; Wang, Chien-Wei
- PLOS Neglected Tropical Diseases, Vol. 13, Issue 5
Field evaluation of a lethal ovitrap against dengue vectors in Brazil
journal, June 2003
- Perich, M. J.; Kardec, A.; Braga, I. A.
- Medical and Veterinary Entomology, Vol. 17, Issue 2
Culturing and Egg Collection of Aedes aegypti
journal, October 2010
- Clemons, A.; Mori, A.; Haugen, M.
- Cold Spring Harbor Protocols, Vol. 2010, Issue 10
Microbial larvicides for mosquito control: Impact of long lasting formulations of Bacillus thuringiensis var. israelensis and Bacillus sphaericus on non‐target organisms in western Kenya highlands
journal, June 2018
- Derua, Yahya A.; Kahindi, Samuel C.; Mosha, Franklin W.
- Ecology and Evolution, Vol. 8, Issue 15