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Title: Evaluation of large volume yeast interfering RNA lure-and-kill ovitraps for attraction and control of Aedes mosquitoes

Abstract

Aedes mosquitoes (Diptera: Culicidae), principle vectors of several arboviruses, typically lay eggs in man-made water-filled containers located near human dwellings. Given the widespread emergence of insecticide resistance, stable and biofriendly alternatives for mosquito larviciding are needed. Laboratory studies have demonstrated that inactivated yeast interfering RNA tablets targeting key larval developmental genes can be used to facilitate effective larvicidal activity while also promoting selective gravid female oviposition behaviour. In this work, we examined the efficacy of transferring this technology toward development of lure-and-kill ovitraps targeting Aedes aegypti (L.) and Aedes albopictus (Skuse) female mosquitoes. Insectary, simulated field and semi-field experiments demonstrated that two mosquito-specific yeast interfering RNA pesticides induce high levels of mortality among larvae of both species in treated large volume containers. Small-scale field trials conducted in Trinidad, West Indies demonstrated that large volume ovitrap containers baited with inactivated yeast tablets lure significantly more gravid females than traps containing only water and were highly attractive to both A. aegypti and A. albopictus females. Finally, these studies indicate that development of biorational yeast interfering RNA-baited ovitraps may represent a new tool for control of Aedes mosquitoes, including deployment in existing lure-and-kill ovitrap technologies or traditional container larviciding programs.

Authors:
 [1];  [1];  [2];  [3];  [1];  [1];  [1];  [2]; ORCiD logo [4];  [2];  [1];  [5]
  1. Indiana Univ. School of Medicine, South Bend, IN (United States); Univ. of Notre Dame, IN (United States)
  2. Univ. of the West Indies, St. Augustine (Trinidad and Tobago)
  3. Univ. of Notre Dame, IN (United States)
  4. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  5. Indiana Univ. School of Medicine, South Bend, IN (United States); Univ. of Notre Dame, IN (United States); Univ. of the West Indies, St. Augustine (Trinidad and Tobago)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE; National Institutes of Health (NIH); USDOD
OSTI Identifier:
1756832
Report Number(s):
LA-UR-20-29552
Journal ID: ISSN 0269-283X
Grant/Contract Number:  
89233218CNA000001; R21 AI128116-01; W81XWH-17-1-0294; W81XWH-17-1-0295
Resource Type:
Accepted Manuscript
Journal Name:
Medical and Veterinary Entomology (Print)
Additional Journal Information:
Journal Name: Medical and Veterinary Entomology (Print); Journal Volume: 35; Journal Issue: 3; Journal ID: ISSN 0269-283X
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; biological science; Aedes aegypti; Aedes albopictus; arbovirus; behaviour; larvicide; oviposition; Trinidad West Indies

Citation Formats

Hapairi, Limb, Mysore, Keshava, James, Lester, Scheel, Nicholas, Realey, Jacob, Sun, Longhua, Gerber, Laura, Shui Feng, Rachel, Romero-Severson, Ethan, Mohammed, Azad, Duman-Scheel, Molly, and Severson, David. Evaluation of large volume yeast interfering RNA lure-and-kill ovitraps for attraction and control of Aedes mosquitoes. United States: N. p., 2020. Web. https://doi.org/10.1111/mve.12504.
Hapairi, Limb, Mysore, Keshava, James, Lester, Scheel, Nicholas, Realey, Jacob, Sun, Longhua, Gerber, Laura, Shui Feng, Rachel, Romero-Severson, Ethan, Mohammed, Azad, Duman-Scheel, Molly, & Severson, David. Evaluation of large volume yeast interfering RNA lure-and-kill ovitraps for attraction and control of Aedes mosquitoes. United States. https://doi.org/10.1111/mve.12504
Hapairi, Limb, Mysore, Keshava, James, Lester, Scheel, Nicholas, Realey, Jacob, Sun, Longhua, Gerber, Laura, Shui Feng, Rachel, Romero-Severson, Ethan, Mohammed, Azad, Duman-Scheel, Molly, and Severson, David. Wed . "Evaluation of large volume yeast interfering RNA lure-and-kill ovitraps for attraction and control of Aedes mosquitoes". United States. https://doi.org/10.1111/mve.12504. https://www.osti.gov/servlets/purl/1756832.
@article{osti_1756832,
title = {Evaluation of large volume yeast interfering RNA lure-and-kill ovitraps for attraction and control of Aedes mosquitoes},
author = {Hapairi, Limb and Mysore, Keshava and James, Lester and Scheel, Nicholas and Realey, Jacob and Sun, Longhua and Gerber, Laura and Shui Feng, Rachel and Romero-Severson, Ethan and Mohammed, Azad and Duman-Scheel, Molly and Severson, David},
abstractNote = {Aedes mosquitoes (Diptera: Culicidae), principle vectors of several arboviruses, typically lay eggs in man-made water-filled containers located near human dwellings. Given the widespread emergence of insecticide resistance, stable and biofriendly alternatives for mosquito larviciding are needed. Laboratory studies have demonstrated that inactivated yeast interfering RNA tablets targeting key larval developmental genes can be used to facilitate effective larvicidal activity while also promoting selective gravid female oviposition behaviour. In this work, we examined the efficacy of transferring this technology toward development of lure-and-kill ovitraps targeting Aedes aegypti (L.) and Aedes albopictus (Skuse) female mosquitoes. Insectary, simulated field and semi-field experiments demonstrated that two mosquito-specific yeast interfering RNA pesticides induce high levels of mortality among larvae of both species in treated large volume containers. Small-scale field trials conducted in Trinidad, West Indies demonstrated that large volume ovitrap containers baited with inactivated yeast tablets lure significantly more gravid females than traps containing only water and were highly attractive to both A. aegypti and A. albopictus females. Finally, these studies indicate that development of biorational yeast interfering RNA-baited ovitraps may represent a new tool for control of Aedes mosquitoes, including deployment in existing lure-and-kill ovitrap technologies or traditional container larviciding programs.},
doi = {10.1111/mve.12504},
journal = {Medical and Veterinary Entomology (Print)},
number = 3,
volume = 35,
place = {United States},
year = {2020},
month = {12}
}

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