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Title: Managing flowering time in Miscanthus and sugarcane to facilitate intra- and intergeneric crosses

Abstract

Miscanthus is a close relative of Saccharum and a potentially valuable genetic resource for improving sugarcane. Differences in flowering time within and between Miscanthus and Saccharum hinders intra- and interspecific hybridizations. A series of greenhouse experiments were conducted over three years to determine how to synchronize flowering time of Saccharum and Miscanthus genotypes. We found that day length was an important factor influencing when Miscanthus and Saccharum flowered. Sugarcane could be induced to flower in a central Illinois greenhouse using supplemental lighting to reduce the rate at which days shortened during the autumn and winter to 1 min d -1 , which allowed us to synchronize the flowering of some sugarcane genotypes with Miscanthus genotypes primarily from low latitudes. In a complementary growth chamber experiment, we evaluated 33 Miscanthus genotypes, including 28 M . sinensis , 2 M . floridulus , and 3 M . ×giganteus collected from 20.9° S to 44.9° N for response to three day lengths (10 h, 12.5 h, and 15 h). High latitude-adapted M . sinensis flowered mainly under 15 h days, but unexpectedly, short days resulted in short, stocky plants that did not flower; in some cases, flag leaves developed under short days butmore » heading did not occur. In contrast, for M . sinensis and M . floridulus from low latitudes, shorter day lengths typically resulted in earlier flowering, and for some low latitude genotypes, 15 h days resulted in no flowering. However, the highest ratio of reproductive shoots to total number of culms was typically observed for 12.5 h or 15 h days. Latitude of origin was significantly associated with culm length, and the shorter the days, the stronger the relationship. Nearly all entries achieved maximal culm length under the 15 h treatment, but the nearer to the equator an accession originated, the less of a difference in culm length between the short-day treatments and the 15 h day treatment. Under short days, short culms for high-latitude accessions was achieved by different physiological mechanisms for M . sinensis genetic groups from the mainland in comparison to those from Japan; for mainland accessions, the mechanism was reduced internode length, whereas for Japanese accessions the phyllochron under short days was greater than under long days. Thus, for M . sinensis , short days typically hastened floral induction, consistent with the expectations for a facultative short-day plant. However, for high latitude accessions of M . sinensis , days less than 12.5 h also signaled that plants should prepare for winter by producing many short culms with limited elongation and development; moreover, this response was also epistatic to flowering. Thus, to flower M . sinensis that originates from high latitudes synchronously with sugarcane, the former needs day lengths >12.5 h (perhaps as high as 15 h), whereas that the latter needs day lengths <12.5 h.« less


Citation Formats

Dong, Hongxu, Clark, Lindsay V., Jin, Xiaoli, Anzoua, Kossonou, Bagmet, Larisa, Chebukin, Pavel, Dzyubenko, Elena, Dzyubenko, Nicolay, Ghimire, Bimal Kumar, Heo, Kweon, Johnson, Douglas A., Nagano, Hironori, Sabitov, Andrey, Peng, Junhua, Yamada, Toshihiko, Yoo, Ji Hye, Yu, Chang Yeon, Zhao, Hua, Long, Stephen P., Sacks, Erik J., and Chiang, ed., Tzen-Yuh. Managing flowering time in Miscanthus and sugarcane to facilitate intra- and intergeneric crosses. United States: N. p., 2021. Web. https://doi.org/10.1371/journal.pone.0240390.
Dong, Hongxu, Clark, Lindsay V., Jin, Xiaoli, Anzoua, Kossonou, Bagmet, Larisa, Chebukin, Pavel, Dzyubenko, Elena, Dzyubenko, Nicolay, Ghimire, Bimal Kumar, Heo, Kweon, Johnson, Douglas A., Nagano, Hironori, Sabitov, Andrey, Peng, Junhua, Yamada, Toshihiko, Yoo, Ji Hye, Yu, Chang Yeon, Zhao, Hua, Long, Stephen P., Sacks, Erik J., & Chiang, ed., Tzen-Yuh. Managing flowering time in Miscanthus and sugarcane to facilitate intra- and intergeneric crosses. United States. https://doi.org/10.1371/journal.pone.0240390
Dong, Hongxu, Clark, Lindsay V., Jin, Xiaoli, Anzoua, Kossonou, Bagmet, Larisa, Chebukin, Pavel, Dzyubenko, Elena, Dzyubenko, Nicolay, Ghimire, Bimal Kumar, Heo, Kweon, Johnson, Douglas A., Nagano, Hironori, Sabitov, Andrey, Peng, Junhua, Yamada, Toshihiko, Yoo, Ji Hye, Yu, Chang Yeon, Zhao, Hua, Long, Stephen P., Sacks, Erik J., and Chiang, ed., Tzen-Yuh. Thu . "Managing flowering time in Miscanthus and sugarcane to facilitate intra- and intergeneric crosses". United States. https://doi.org/10.1371/journal.pone.0240390.
@article{osti_1756133,
title = {Managing flowering time in Miscanthus and sugarcane to facilitate intra- and intergeneric crosses},
author = {Dong, Hongxu and Clark, Lindsay V. and Jin, Xiaoli and Anzoua, Kossonou and Bagmet, Larisa and Chebukin, Pavel and Dzyubenko, Elena and Dzyubenko, Nicolay and Ghimire, Bimal Kumar and Heo, Kweon and Johnson, Douglas A. and Nagano, Hironori and Sabitov, Andrey and Peng, Junhua and Yamada, Toshihiko and Yoo, Ji Hye and Yu, Chang Yeon and Zhao, Hua and Long, Stephen P. and Sacks, Erik J. and Chiang, ed., Tzen-Yuh},
abstractNote = {Miscanthus is a close relative of Saccharum and a potentially valuable genetic resource for improving sugarcane. Differences in flowering time within and between Miscanthus and Saccharum hinders intra- and interspecific hybridizations. A series of greenhouse experiments were conducted over three years to determine how to synchronize flowering time of Saccharum and Miscanthus genotypes. We found that day length was an important factor influencing when Miscanthus and Saccharum flowered. Sugarcane could be induced to flower in a central Illinois greenhouse using supplemental lighting to reduce the rate at which days shortened during the autumn and winter to 1 min d -1 , which allowed us to synchronize the flowering of some sugarcane genotypes with Miscanthus genotypes primarily from low latitudes. In a complementary growth chamber experiment, we evaluated 33 Miscanthus genotypes, including 28 M . sinensis , 2 M . floridulus , and 3 M . ×giganteus collected from 20.9° S to 44.9° N for response to three day lengths (10 h, 12.5 h, and 15 h). High latitude-adapted M . sinensis flowered mainly under 15 h days, but unexpectedly, short days resulted in short, stocky plants that did not flower; in some cases, flag leaves developed under short days but heading did not occur. In contrast, for M . sinensis and M . floridulus from low latitudes, shorter day lengths typically resulted in earlier flowering, and for some low latitude genotypes, 15 h days resulted in no flowering. However, the highest ratio of reproductive shoots to total number of culms was typically observed for 12.5 h or 15 h days. Latitude of origin was significantly associated with culm length, and the shorter the days, the stronger the relationship. Nearly all entries achieved maximal culm length under the 15 h treatment, but the nearer to the equator an accession originated, the less of a difference in culm length between the short-day treatments and the 15 h day treatment. Under short days, short culms for high-latitude accessions was achieved by different physiological mechanisms for M . sinensis genetic groups from the mainland in comparison to those from Japan; for mainland accessions, the mechanism was reduced internode length, whereas for Japanese accessions the phyllochron under short days was greater than under long days. Thus, for M . sinensis , short days typically hastened floral induction, consistent with the expectations for a facultative short-day plant. However, for high latitude accessions of M . sinensis , days less than 12.5 h also signaled that plants should prepare for winter by producing many short culms with limited elongation and development; moreover, this response was also epistatic to flowering. Thus, to flower M . sinensis that originates from high latitudes synchronously with sugarcane, the former needs day lengths >12.5 h (perhaps as high as 15 h), whereas that the latter needs day lengths <12.5 h.},
doi = {10.1371/journal.pone.0240390},
journal = {PLoS ONE},
number = 1,
volume = 16,
place = {United States},
year = {2021},
month = {1}
}

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