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Title: Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial

Abstract

Background: Circulating tumour cells (CTCs) represent a morphologically distinct subset of cancer cells, which aid the metastatic spread. The ExPeCT trial aimed to examine the effectiveness of a structured exercise programme in modulating levels of CTCs and platelet cloaking in patients with metastatic prostate cancer. Methods: Participants (n = 61) were randomised into either standard care (control) or exercise arms. Whole blood was collected for all participants at baseline (T0), three months (T3) and six months (T6), and analysed for the presence of CTCs, CTC clusters and platelet cloaking. CTC data was correlated with clinico-pathological information. Results: Changes in CTC number were observed within group over time, however no significant difference in CTC number was observed between groups over time. Platelet cloaking was identified in 29.5% of participants. A positive correlation between CTC number and white cell count (WCC) was observed (p = 0.0001), in addition to a positive relationship between CTC clusters and PSA levels (p = 0.0393). Conclusion: The presence of platelet cloaking has been observed in this patient population for the first time, in addition to a significant correlation between CTC number and WCC. Trial registration: ClincalTrials.gov identifier NCT02453139.

Authors:
ORCiD logo [1];  [2];  [1];  [1];  [1]; ORCiD logo [3];  [4];  [5];  [5];  [1];  [6];  [1];  [7];  [7];  [8];  [9];  [10];  [11];  [12]; ORCiD logo [13] more »;  [14];  [12];  [1];  [12];  [8];  [15];  [16] « less
  1. Trinity College Dublin (Ireland)
  2. Cork Univ. Hospital (Ireland); Univ. College Cork (Ireland)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  4. Univ. Hospital Basel (Switzerland)
  5. Cancer Trials Ireland, Dublin (Ireland)
  6. Trinity College Dublin (Ireland); Queen's Univ., Belfast, Northern Ireland (United Kingdom)
  7. King's College, London (United Kingdom)
  8. Guy's and St. Thomas' NHS Foundation Trust, London (United Kingdom)
  9. Harvard Univ., Cambridge, MA (United States). T. H. Chan School of Public Health
  10. St. Luke's Hospital, Dublin (Ireland)
  11. Beaumont Hospital, Dublin (Ireland)
  12. St. James's Hospital, Dublin (Ireland)
  13. St. James's Hospital, Dublin (Ireland); Trinity College Dublin (Ireland)
  14. Mater Misericordiae Hospital, Dublin (Ireland)
  15. Tallaght Univ. Hospital, Dublin (Ireland)
  16. Trinity College Dublin (Ireland); Cancer Trials Ireland, Dublin (Ireland); St. James's Hospital, Dublin (Ireland)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1755927
Report Number(s):
PNNL-ACT-SA-10402
Journal ID: ISSN 1932-6203
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 15; Journal Issue: 12; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Brady, Lauren, Hayes, Brian, Sheill, Gráinne, Baird, Anne-Marie, Guinan, Emer, Stanfill, Bryan, Vlajnic, Tatjana, Casey, Orla, Murphy, Verena, Greene, John, Allott, Emma H., Hussey, Juliette, Cahill, Fidelma, Van Hemelrijck, Mieke, Peat, Nicola, Mucci, Lorelei, Cunningham, Moya, Grogan, Liam, Lynch, Thomas, Manecksha, Rustom P., McCaffrey, John, O’Donnell, Dearbhaile, Sheils, Orla, O’Leary, John, Rudman, Sarah, McDermott, Ray, and Finn, Stephen. Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial. United States: N. p., 2020. Web. https://doi.org/10.1371/journal.pone.0243928.
Brady, Lauren, Hayes, Brian, Sheill, Gráinne, Baird, Anne-Marie, Guinan, Emer, Stanfill, Bryan, Vlajnic, Tatjana, Casey, Orla, Murphy, Verena, Greene, John, Allott, Emma H., Hussey, Juliette, Cahill, Fidelma, Van Hemelrijck, Mieke, Peat, Nicola, Mucci, Lorelei, Cunningham, Moya, Grogan, Liam, Lynch, Thomas, Manecksha, Rustom P., McCaffrey, John, O’Donnell, Dearbhaile, Sheils, Orla, O’Leary, John, Rudman, Sarah, McDermott, Ray, & Finn, Stephen. Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial. United States. https://doi.org/10.1371/journal.pone.0243928
Brady, Lauren, Hayes, Brian, Sheill, Gráinne, Baird, Anne-Marie, Guinan, Emer, Stanfill, Bryan, Vlajnic, Tatjana, Casey, Orla, Murphy, Verena, Greene, John, Allott, Emma H., Hussey, Juliette, Cahill, Fidelma, Van Hemelrijck, Mieke, Peat, Nicola, Mucci, Lorelei, Cunningham, Moya, Grogan, Liam, Lynch, Thomas, Manecksha, Rustom P., McCaffrey, John, O’Donnell, Dearbhaile, Sheils, Orla, O’Leary, John, Rudman, Sarah, McDermott, Ray, and Finn, Stephen. Fri . "Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial". United States. https://doi.org/10.1371/journal.pone.0243928. https://www.osti.gov/servlets/purl/1755927.
@article{osti_1755927,
title = {Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial},
author = {Brady, Lauren and Hayes, Brian and Sheill, Gráinne and Baird, Anne-Marie and Guinan, Emer and Stanfill, Bryan and Vlajnic, Tatjana and Casey, Orla and Murphy, Verena and Greene, John and Allott, Emma H. and Hussey, Juliette and Cahill, Fidelma and Van Hemelrijck, Mieke and Peat, Nicola and Mucci, Lorelei and Cunningham, Moya and Grogan, Liam and Lynch, Thomas and Manecksha, Rustom P. and McCaffrey, John and O’Donnell, Dearbhaile and Sheils, Orla and O’Leary, John and Rudman, Sarah and McDermott, Ray and Finn, Stephen},
abstractNote = {Background: Circulating tumour cells (CTCs) represent a morphologically distinct subset of cancer cells, which aid the metastatic spread. The ExPeCT trial aimed to examine the effectiveness of a structured exercise programme in modulating levels of CTCs and platelet cloaking in patients with metastatic prostate cancer. Methods: Participants (n = 61) were randomised into either standard care (control) or exercise arms. Whole blood was collected for all participants at baseline (T0), three months (T3) and six months (T6), and analysed for the presence of CTCs, CTC clusters and platelet cloaking. CTC data was correlated with clinico-pathological information. Results: Changes in CTC number were observed within group over time, however no significant difference in CTC number was observed between groups over time. Platelet cloaking was identified in 29.5% of participants. A positive correlation between CTC number and white cell count (WCC) was observed (p = 0.0001), in addition to a positive relationship between CTC clusters and PSA levels (p = 0.0393). Conclusion: The presence of platelet cloaking has been observed in this patient population for the first time, in addition to a significant correlation between CTC number and WCC. Trial registration: ClincalTrials.gov identifier NCT02453139.},
doi = {10.1371/journal.pone.0243928},
journal = {PLoS ONE},
number = 12,
volume = 15,
place = {United States},
year = {2020},
month = {12}
}

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