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Title: The Model System Saccharomyces cerevisiae Versus Emerging Non-Model Yeasts for the Production of Biofuels

Abstract

Microorganisms are effective platforms for the production of a variety of chemicals including biofuels, commodity chemicals, polymers and other natural products. However, deep cellular understanding is required for improvement of current biofuel cell factories to truly transform the Bioeconomy. Modifications in microbial metabolic pathways and increased resistance to various types of stress caused by the production of these chemicals are crucial in the generation of robust and efficient production hosts. Recent advances in systems and synthetic biology provide new tools for metabolic engineering to design strategies and construct optimal biocatalysts for the sustainable production of desired chemicals, especially in the case of ethanol and fatty acid production. Yeast is an efficient producer of bioethanol and most of the available synthetic biology tools have been developed for the industrial yeast Saccharomyces cerevisiae. Non-conventional yeast systems have several advantageous characteristics that are not easily engineered such as ethanol tolerance, low pH tolerance, thermotolerance, inhibitor tolerance, genetic diversity and so forth. Currently, synthetic biology is still in its initial steps for studies in non-conventional yeasts such as Yarrowia lipolytica, Kluyveromyces marxianus, Issatchenkia orientalis and Pichia pastoris. Therefore, the development and application of advanced synthetic engineering tools must also focus on these underexploited, non-conventionalmore » yeast species. Herein, we review the basic synthetic biology tools that can be applied to the standard S. cerevisiae model strain, as well as those that have been developed for non-conventional yeasts. In addition, we will discuss the recent advances employed to develop non-conventional yeast strains that are efficient for the production of a variety of chemicals through the use of metabolic engineering and synthetic biology.« less

Authors:
 [1];  [2]; ORCiD logo [3]
  1. Univ. of Colorado, Boulder, CO (United States)
  2. Purdue Univ., West Lafayette, IN (United States)
  3. Univ. of Colorado, Boulder, CO (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
OSTI Identifier:
1755690
Report Number(s):
NREL/JA-2700-78416
Journal ID: ISSN 2075-1729; MainId:32333;UUID:65df2d32-85ce-46cc-9869-48ca074bc6e7;MainAdminID:18857
Grant/Contract Number:  
AC36-08GO28308; SC0018368
Resource Type:
Accepted Manuscript
Journal Name:
Life
Additional Journal Information:
Journal Volume: 10; Journal Issue: 11; Journal ID: ISSN 2075-1729
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; biofuels; bioproducts; metabolic engineering; yeast; synthetic biology

Citation Formats

Lacerda, Maria Priscila, Oh, Eun Joong, and Eckert, Carrie. The Model System Saccharomyces cerevisiae Versus Emerging Non-Model Yeasts for the Production of Biofuels. United States: N. p., 2020. Web. https://doi.org/10.3390/life10110299.
Lacerda, Maria Priscila, Oh, Eun Joong, & Eckert, Carrie. The Model System Saccharomyces cerevisiae Versus Emerging Non-Model Yeasts for the Production of Biofuels. United States. https://doi.org/10.3390/life10110299
Lacerda, Maria Priscila, Oh, Eun Joong, and Eckert, Carrie. Sat . "The Model System Saccharomyces cerevisiae Versus Emerging Non-Model Yeasts for the Production of Biofuels". United States. https://doi.org/10.3390/life10110299. https://www.osti.gov/servlets/purl/1755690.
@article{osti_1755690,
title = {The Model System Saccharomyces cerevisiae Versus Emerging Non-Model Yeasts for the Production of Biofuels},
author = {Lacerda, Maria Priscila and Oh, Eun Joong and Eckert, Carrie},
abstractNote = {Microorganisms are effective platforms for the production of a variety of chemicals including biofuels, commodity chemicals, polymers and other natural products. However, deep cellular understanding is required for improvement of current biofuel cell factories to truly transform the Bioeconomy. Modifications in microbial metabolic pathways and increased resistance to various types of stress caused by the production of these chemicals are crucial in the generation of robust and efficient production hosts. Recent advances in systems and synthetic biology provide new tools for metabolic engineering to design strategies and construct optimal biocatalysts for the sustainable production of desired chemicals, especially in the case of ethanol and fatty acid production. Yeast is an efficient producer of bioethanol and most of the available synthetic biology tools have been developed for the industrial yeast Saccharomyces cerevisiae. Non-conventional yeast systems have several advantageous characteristics that are not easily engineered such as ethanol tolerance, low pH tolerance, thermotolerance, inhibitor tolerance, genetic diversity and so forth. Currently, synthetic biology is still in its initial steps for studies in non-conventional yeasts such as Yarrowia lipolytica, Kluyveromyces marxianus, Issatchenkia orientalis and Pichia pastoris. Therefore, the development and application of advanced synthetic engineering tools must also focus on these underexploited, non-conventional yeast species. Herein, we review the basic synthetic biology tools that can be applied to the standard S. cerevisiae model strain, as well as those that have been developed for non-conventional yeasts. In addition, we will discuss the recent advances employed to develop non-conventional yeast strains that are efficient for the production of a variety of chemicals through the use of metabolic engineering and synthetic biology.},
doi = {10.3390/life10110299},
journal = {Life},
number = 11,
volume = 10,
place = {United States},
year = {2020},
month = {11}
}

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