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Title: Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus

Abstract

We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention’s Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States.

Authors:
ORCiD logo [1];  [2];  [3];  [1];  [1];  [1];
  1. Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
  2. Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA
  3. IHRC Incorporated, Atlanta, Georgia, USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1742077
Resource Type:
Published Article
Journal Name:
Journal of Clinical Microbiology
Additional Journal Information:
Journal Name: Journal of Clinical Microbiology Journal Volume: 59 Journal Issue: 1; Journal ID: ISSN 0095-1137
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English

Citation Formats

Velusamy, Srinivasan, Jordak, Katherine, Kupor, Madeline, Chochua, Sopio, McGee, Lesley, Beall, Bernard, and Burnham, ed., Carey-Ann D.. Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus. United States: N. p., 2020. Web. https://doi.org/10.1128/JCM.01764-20.
Velusamy, Srinivasan, Jordak, Katherine, Kupor, Madeline, Chochua, Sopio, McGee, Lesley, Beall, Bernard, & Burnham, ed., Carey-Ann D.. Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus. United States. https://doi.org/10.1128/JCM.01764-20
Velusamy, Srinivasan, Jordak, Katherine, Kupor, Madeline, Chochua, Sopio, McGee, Lesley, Beall, Bernard, and Burnham, ed., Carey-Ann D.. Thu . "Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus". United States. https://doi.org/10.1128/JCM.01764-20.
@article{osti_1742077,
title = {Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus},
author = {Velusamy, Srinivasan and Jordak, Katherine and Kupor, Madeline and Chochua, Sopio and McGee, Lesley and Beall, Bernard and Burnham, ed., Carey-Ann D.},
abstractNote = {We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention’s Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States.},
doi = {10.1128/JCM.01764-20},
journal = {Journal of Clinical Microbiology},
number = 1,
volume = 59,
place = {United States},
year = {2020},
month = {12}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1128/JCM.01764-20

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