Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus

Velusamy, Srinivasan; Jordak, Katherine; Kupor, Madeline; Chochua, Sopio; McGee, Lesley; Beall, Bernard; Burnham, ed., Carey-Ann D.

doi:10.1128/JCM.01764-20

Title: Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus

Journal Article · 17 December 2020 · Journal of Clinical Microbiology

DOI: https://doi.org/10.1128/JCM.01764-20 · OSTI ID:1742077

^[1]; Jordak, Katherine ^[2]; Kupor, Madeline ^[3]; Chochua, Sopio ^[1]; McGee, Lesley ^[1]; Beall, Bernard ^[1]; Burnham, ed., Carey-Ann D.

Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA
IHRC Incorporated, Atlanta, Georgia, USA

We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention’s Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States.

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Sponsoring Organization:: USDOE

OSTI ID:: 1742077

Journal Information:: Journal of Clinical Microbiology, Journal Name: Journal of Clinical Microbiology Vol. 59 Journal Issue: 1; ISSN 0095-1137

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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