Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus
Abstract
We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention’s Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States.
- Authors:
-
[1];
- Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
- Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA
- IHRC Incorporated, Atlanta, Georgia, USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1742077
- Resource Type:
- Published Article
- Journal Name:
- Journal of Clinical Microbiology
- Additional Journal Information:
- Journal Name: Journal of Clinical Microbiology Journal Volume: 59 Journal Issue: 1; Journal ID: ISSN 0095-1137
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Velusamy, Srinivasan, Jordak, Katherine, Kupor, Madeline, Chochua, Sopio, McGee, Lesley, Beall, Bernard, and Burnham, ed., Carey-Ann D. Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus. United States: N. p., 2020.
Web. doi:10.1128/JCM.01764-20.
Velusamy, Srinivasan, Jordak, Katherine, Kupor, Madeline, Chochua, Sopio, McGee, Lesley, Beall, Bernard, & Burnham, ed., Carey-Ann D. Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus. United States. https://doi.org/10.1128/JCM.01764-20
Velusamy, Srinivasan, Jordak, Katherine, Kupor, Madeline, Chochua, Sopio, McGee, Lesley, Beall, Bernard, and Burnham, ed., Carey-Ann D. Thu .
"Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus". United States. https://doi.org/10.1128/JCM.01764-20.
@article{osti_1742077,
title = {Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus},
author = {Velusamy, Srinivasan and Jordak, Katherine and Kupor, Madeline and Chochua, Sopio and McGee, Lesley and Beall, Bernard and Burnham, ed., Carey-Ann D.},
abstractNote = {We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention’s Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States.},
doi = {10.1128/JCM.01764-20},
journal = {Journal of Clinical Microbiology},
number = 1,
volume = 59,
place = {United States},
year = {2020},
month = {12}
}
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https://doi.org/10.1128/JCM.01764-20
https://doi.org/10.1128/JCM.01764-20
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