Title: A plant genetic network for preventing dysbiosis in the phyllosphere

Abstract

The aboveground parts of terrestrial plants, collectively called the phyllosphere, have a key role in the global balance of atmospheric carbon dioxide and oxygen. The phyllosphere represents one of the most abundant habitats for microbiota colonization. Whether and how plants control phyllosphere microbiota to ensure plant health is not well understood. Here we show that the Arabidopsis quadruple mutant (min7 fls2 efr cerk1; hereafter, mfec)1, simultaneously defective in pattern-triggered immunity and the MIN7 vesicle-trafficking pathway, or a constitutively activated cell death1 (cad1) mutant, carrying a S205F mutation in a membrane-attack-complex/perforin (MACPF)-domain protein, harbour altered endophytic phyllosphere microbiota and display leaf-tissue damage associated with dysbiosis. The Shannon diversity index and the relative abundance of Firmicutes were markedly reduced, whereas Proteobacteria were enriched in the mfec and cad1S205F mutants, bearing cross-kingdom resemblance to some aspects of the dysbiosis that occurs in human inflammatory bowel disease. Bacterial community transplantation experiments demonstrated a causal role of a properly assembled leaf bacterial community in phyllosphere health. Here, the pattern-triggered immune signalling, MIN7 and CAD1 are found in major land plant lineages and are probably key components of a genetic network through which terrestrial plants control the level and nurture the diversity of endophytic phyllosphere microbiotamore » for survival and health in a microorganism-rich environment.« less

Authors:

Chen, Tao ^[1]; Nomura, Kinya ^[2]; Wang, Xiaolin ^[3]; Sohrabi, Reza  ^[2];

• Search DOE PAGES for author "Sohrabi, Reza"
• Search DOE PAGES for ORCID "0000-0003-1621-8496"

Xu, Jin  ^[4];

• Search DOE PAGES for author "Xu, Jin"
• Search DOE PAGES for ORCID "0000-0003-2289-4896"

Yao, Lingya ^[3]; Paasch, Bradley C. ^[2]; Ma, Li ^[2]; Kremer, James  ^[2];

• Search DOE PAGES for author "Kremer, James"
• Search DOE PAGES for ORCID "0000-0002-9240-1607"

Cheng, Yuti ^[2]; Zhang, Li  ^[2];

• Search DOE PAGES for author "Zhang, Li"
• Search DOE PAGES for ORCID "0000-0003-3112-7373"

Wang, Nian  ^[4];

• Search DOE PAGES for author "Wang, Nian"
• Search DOE PAGES for ORCID "0000-0001-7743-0728"

Wang, Ertao  ^[3];

• Search DOE PAGES for author "Wang, Ertao"
• Search DOE PAGES for ORCID "0000-0002-5178-3530"

Xin, Xiu-Fang ^[3]; He, Sheng Yang ^[2]

---

+ Show Author Affiliations

1. Michigan State Univ., East Lansing, MI (United States); Huazhong Agricultural Univ., Wuhan (China)
2. Michigan State Univ., East Lansing, MI (United States)
3. Chinese Academy of Sciences, Shanghai (China)
4. Univ. of Florida, Lake Alfred, FL (United States)

Publication Date:

Wed Apr 08 00:00:00 EDT 2020

Research Org.:

Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory

Sponsoring Org.:

USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:

1735817

Grant/Contract Number:  

FG02-91ER20021

Resource Type:

Accepted Manuscript

Journal Name:

Nature (London)

Additional Journal Information:

Journal Name: Nature (London); Journal Volume: 580; Journal Issue: 7805; Journal ID: ISSN 0028-0836

Publisher:

Nature Publishing Group

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES