Building a custom high-throughput platform at the Joint Genome Institute for DNA construct design and assembly—present and future challenges

Journal Article · 04 November 2020 · Synthetic Biology

DOI: https://doi.org/10.1093/synbio/ysaa023 · OSTI ID:1735762

Blaby, Ian K.  ^[1]; Cheng, Jan-Fang  ^[1]

---

1. US Department of Energy, Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA

Abstract The rapid design and assembly of synthetic DNA constructs have become a crucial component of biological engineering projects via iterative design–build–test–learn cycles. In this perspective, we provide an overview of the workflows used to generate the thousands of constructs and libraries produced each year at the U.S. Department of Energy Joint Genome Institute. Particular attention is paid to describing pipelines, tools used, types of scientific projects enabled by the platform and challenges faced in further scaling output.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)
• Grant/Contract Number: AC02-05CH11231
• OSTI ID: 1735762
• Journal Information: Synthetic Biology, Journal Name: Synthetic Biology Journal Issue: 1 Vol. 5; ISSN 2397-7000
• Publisher: Oxford University PressCopyright Statement
• Country of Publication: United Kingdom
• Language: English