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Title: A grass-specific cellulose–xylan interaction dominates in sorghum secondary cell walls

Abstract

Sorghum (Sorghum bicolor L. Moench) is a promising source of lignocellulosic biomass for the production of renewable fuels and chemicals, as well as for forage. Understanding secondary cell wall architecture is key to understanding recalcitrance i.e. identifying features which prevent the efficient conversion of complex biomass to simple carbon units. Here, we use multi-dimensional magic angle spinning solid-state NMR to characterize the sorghum secondary cell wall. We show that xylan is mainly in a three-fold screw conformation due to dense arabinosyl substitutions, with close proximity to cellulose. We also show that sorghum secondary cell walls present a high ratio of amorphous to crystalline cellulose as compared to dicots. We propose a model of sorghum cell wall architecture which is dominated by interactions between three-fold screw xylan and amorphous cellulose. This work will aid the design of low-recalcitrance biomass crops, a requirement for a sustainable bioeconomy.

Authors:
ORCiD logo; ORCiD logo; ; ORCiD logo; ORCiD logo
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1724443
Alternate Identifier(s):
OSTI ID: 1728576; OSTI ID: 1765582
Report Number(s):
PNNL-SA-154449
Journal ID: ISSN 2041-1723; 6081; PII: 19837
Grant/Contract Number:  
AC05-76RL01830; AC02-05CH11231
Resource Type:
Published Article
Journal Name:
Nature Communications
Additional Journal Information:
Journal Name: Nature Communications Journal Volume: 11 Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; sorghum; secondary cell wall; xylan; arabinose; solid-state NMR; biofuels; plant physiology

Citation Formats

Gao, Yu, Lipton, Andrew S., Wittmer, Yuuki, Murray, Dylan T., and Mortimer, Jenny C.. A grass-specific cellulose–xylan interaction dominates in sorghum secondary cell walls. United Kingdom: N. p., 2020. Web. https://doi.org/10.1038/s41467-020-19837-z.
Gao, Yu, Lipton, Andrew S., Wittmer, Yuuki, Murray, Dylan T., & Mortimer, Jenny C.. A grass-specific cellulose–xylan interaction dominates in sorghum secondary cell walls. United Kingdom. https://doi.org/10.1038/s41467-020-19837-z
Gao, Yu, Lipton, Andrew S., Wittmer, Yuuki, Murray, Dylan T., and Mortimer, Jenny C.. Fri . "A grass-specific cellulose–xylan interaction dominates in sorghum secondary cell walls". United Kingdom. https://doi.org/10.1038/s41467-020-19837-z.
@article{osti_1724443,
title = {A grass-specific cellulose–xylan interaction dominates in sorghum secondary cell walls},
author = {Gao, Yu and Lipton, Andrew S. and Wittmer, Yuuki and Murray, Dylan T. and Mortimer, Jenny C.},
abstractNote = {Sorghum (Sorghum bicolor L. Moench) is a promising source of lignocellulosic biomass for the production of renewable fuels and chemicals, as well as for forage. Understanding secondary cell wall architecture is key to understanding recalcitrance i.e. identifying features which prevent the efficient conversion of complex biomass to simple carbon units. Here, we use multi-dimensional magic angle spinning solid-state NMR to characterize the sorghum secondary cell wall. We show that xylan is mainly in a three-fold screw conformation due to dense arabinosyl substitutions, with close proximity to cellulose. We also show that sorghum secondary cell walls present a high ratio of amorphous to crystalline cellulose as compared to dicots. We propose a model of sorghum cell wall architecture which is dominated by interactions between three-fold screw xylan and amorphous cellulose. This work will aid the design of low-recalcitrance biomass crops, a requirement for a sustainable bioeconomy.},
doi = {10.1038/s41467-020-19837-z},
journal = {Nature Communications},
number = 1,
volume = 11,
place = {United Kingdom},
year = {2020},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1038/s41467-020-19837-z

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