A grass-specific cellulose–xylan interaction dominates in sorghum secondary cell walls

Gao, Yu; Lipton, Andrew S.; Wittmer, Yuuki; Murray, Dylan T.; Mortimer, Jenny C.

doi:10.1038/s41467-020-19837-z

Title: A grass-specific cellulose–xylan interaction dominates in sorghum secondary cell walls

Journal Article · 27 November 2020 · Nature Communications

DOI: https://doi.org/10.1038/s41467-020-19837-z · OSTI ID:1724443

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Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Univ. of California, Davis, CA (United States)
Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of Adelaide, SA (Australia)

Sorghum (Sorghum bicolor L. Moench) is a promising source of lignocellulosic biomass for the production of renewable fuels and chemicals, as well as for forage. Understanding secondary cell wall architecture is key to understanding recalcitrance i.e. identifying features which prevent the efficient conversion of complex biomass to simple carbon units. Here, we use multi-dimensional magic angle spinning solid-state NMR to characterize the sorghum secondary cell wall. We show that xylan is mainly in a three-fold screw conformation due to dense arabinosyl substitutions, with close proximity to cellulose. We also show that sorghum secondary cell walls present a high ratio of amorphous to crystalline cellulose as compared to dicots. We propose a model of sorghum cell wall architecture which is dominated by interactions between three-fold screw xylan and amorphous cellulose. This work will aid the design of low-recalcitrance biomass crops, a requirement for a sustainable bioeconomy.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; AC02-05CH11231

OSTI ID:: 1724443

Alternate ID(s):: OSTI ID: 1728576; OSTI ID: 1765582

Report Number(s):: PNNL-SA-154449

Journal Information:: Nature Communications, Vol. 11, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
sorghum
secondary cell wall
xylan
arabinose
solid-state NMR
biofuels
plant physiology

Title: A grass-specific cellulose–xylan interaction dominates in sorghum secondary cell walls

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