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Title: High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

Abstract

Abstract Understanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.

Authors:
ORCiD logo; ; ; ; ; ; ORCiD logo
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); USDOE Laboratory Directed Research and Development (LDRD) Program; National Institutes of Health (NIH)
OSTI Identifier:
1724291
Alternate Identifier(s):
OSTI ID: 1763690
Grant/Contract Number:  
AC02-05CH11231; LDRD 20-116; U01NS103489
Resource Type:
Published Article
Journal Name:
Nature Communications
Additional Journal Information:
Journal Name: Nature Communications Journal Volume: 11 Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Fan, Jiang Lan, Rivera, Jose A., Sun, Wei, Peterson, John, Haeberle, Henry, Rubin, Sam, and Ji, Na. High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics. United Kingdom: N. p., 2020. Web. https://doi.org/10.1038/s41467-020-19851-1.
Fan, Jiang Lan, Rivera, Jose A., Sun, Wei, Peterson, John, Haeberle, Henry, Rubin, Sam, & Ji, Na. High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics. United Kingdom. https://doi.org/10.1038/s41467-020-19851-1
Fan, Jiang Lan, Rivera, Jose A., Sun, Wei, Peterson, John, Haeberle, Henry, Rubin, Sam, and Ji, Na. Thu . "High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics". United Kingdom. https://doi.org/10.1038/s41467-020-19851-1.
@article{osti_1724291,
title = {High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics},
author = {Fan, Jiang Lan and Rivera, Jose A. and Sun, Wei and Peterson, John and Haeberle, Henry and Rubin, Sam and Ji, Na},
abstractNote = {Abstract Understanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.},
doi = {10.1038/s41467-020-19851-1},
journal = {Nature Communications},
number = 1,
volume = 11,
place = {United Kingdom},
year = {2020},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1038/s41467-020-19851-1

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