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Title: Insights into community of photosynthetic microorganisms from permafrost

Abstract

ABSTRACT This work integrates cultivation studies of Siberian permafrost and analyses of metagenomes from different locations in the Arctic with the aim of obtaining insights into the community of photosynthetic microorganisms in perennially frozen deposits. Cyanobacteria and microalgae have been described in Arctic aquatic and surface soil environments, but their diversity and ability to withstand harsh conditions within the permafrost are still largely unknown. Community structure of photosynthetic organisms in permafrost sediments was explored using Arctic metagenomes available through the MG-RAST. Sequences affiliated with cyanobacteria represented from 0.25 to 3.03% of total sequences, followed by sequences affiliated with Streptophyta (algae and vascular plants) 0.01–0.45% and Chlorophyta (green algae) 0.01–0.1%. Enrichment and cultivation approaches revealed that cyanobacteria and green algae survive in permafrost and they could be revived during prolonged incubation at low light intensity. Among photosynthetic microorganisms isolated from permafrost, the filamentous Oscillatoria-like cyanobacteria and unicellular green algae of the genus Chlorella were dominant. Our findings suggest that permafrost cyanobacteria and green algae are expected to be effective members of the re-assembled community after permafrost thawing and soil collapse.

Authors:
ORCiD logo [1];  [2];  [3];  [2];  [2];  [2]; ORCiD logo [3]
  1. Center for Environmental Biotechnology, University of Tennessee, 676 Dabney Hall, 1416 Circle Drive, Knoxville, TN 37996-1605, USA, Soil Cryology Laboratory, Institute of Physicochemical and Biological Problems in Soil Science, Institutskaya Street, Bldg. 2, Pushchino, Russia
  2. Center for Environmental Biotechnology, University of Tennessee, 676 Dabney Hall, 1416 Circle Drive, Knoxville, TN 37996-1605, USA
  3. Soil Cryology Laboratory, Institute of Physicochemical and Biological Problems in Soil Science, Institutskaya Street, Bldg. 2, Pushchino, Russia
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1724278
Grant/Contract Number:  
SC0020369
Resource Type:
Published Article
Journal Name:
FEMS Microbiology Ecology
Additional Journal Information:
Journal Name: FEMS Microbiology Ecology Journal Volume: 96 Journal Issue: 12; Journal ID: ISSN 0168-6496
Publisher:
Oxford University Press
Country of Publication:
Netherlands
Language:
English

Citation Formats

Vishnivetskaya, Tatiana A., Almatari, Abraham L., Spirina, Elena V., Wu, Xiaofen, Williams, Daniel E., Pfiffner, Susan M., and Rivkina, Elizaveta M.. Insights into community of photosynthetic microorganisms from permafrost. Netherlands: N. p., 2020. Web. https://doi.org/10.1093/femsec/fiaa229.
Vishnivetskaya, Tatiana A., Almatari, Abraham L., Spirina, Elena V., Wu, Xiaofen, Williams, Daniel E., Pfiffner, Susan M., & Rivkina, Elizaveta M.. Insights into community of photosynthetic microorganisms from permafrost. Netherlands. https://doi.org/10.1093/femsec/fiaa229
Vishnivetskaya, Tatiana A., Almatari, Abraham L., Spirina, Elena V., Wu, Xiaofen, Williams, Daniel E., Pfiffner, Susan M., and Rivkina, Elizaveta M.. Thu . "Insights into community of photosynthetic microorganisms from permafrost". Netherlands. https://doi.org/10.1093/femsec/fiaa229.
@article{osti_1724278,
title = {Insights into community of photosynthetic microorganisms from permafrost},
author = {Vishnivetskaya, Tatiana A. and Almatari, Abraham L. and Spirina, Elena V. and Wu, Xiaofen and Williams, Daniel E. and Pfiffner, Susan M. and Rivkina, Elizaveta M.},
abstractNote = {ABSTRACT This work integrates cultivation studies of Siberian permafrost and analyses of metagenomes from different locations in the Arctic with the aim of obtaining insights into the community of photosynthetic microorganisms in perennially frozen deposits. Cyanobacteria and microalgae have been described in Arctic aquatic and surface soil environments, but their diversity and ability to withstand harsh conditions within the permafrost are still largely unknown. Community structure of photosynthetic organisms in permafrost sediments was explored using Arctic metagenomes available through the MG-RAST. Sequences affiliated with cyanobacteria represented from 0.25 to 3.03% of total sequences, followed by sequences affiliated with Streptophyta (algae and vascular plants) 0.01–0.45% and Chlorophyta (green algae) 0.01–0.1%. Enrichment and cultivation approaches revealed that cyanobacteria and green algae survive in permafrost and they could be revived during prolonged incubation at low light intensity. Among photosynthetic microorganisms isolated from permafrost, the filamentous Oscillatoria-like cyanobacteria and unicellular green algae of the genus Chlorella were dominant. Our findings suggest that permafrost cyanobacteria and green algae are expected to be effective members of the re-assembled community after permafrost thawing and soil collapse.},
doi = {10.1093/femsec/fiaa229},
journal = {FEMS Microbiology Ecology},
number = 12,
volume = 96,
place = {Netherlands},
year = {2020},
month = {11}
}

Journal Article:
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https://doi.org/10.1093/femsec/fiaa229

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