The effects of osmolytes on in vitro kinesin-microtubule motility assays

VanDelinder, Virginia; Sickafoose, Ian; Imam, Zachary I.; Ko, Randy; Bachand, George D.

doi:10.1039/D0RA08148E

Title: The effects of osmolytes on in vitro kinesin-microtubule motility assays

Abstract

Kinesin-driven motility was shown to be adversely affected in a concentration dependent manner by the addition of osmolytes: glycerol, polyethylene glycol, and trimethylamine N -oxide.

Authors:

VanDelinder, Virginia ^[1]; Sickafoose, Ian ^[1]; Imam, Zachary I. ^[1]; Ko, Randy ^[1];

^[1]

Center for Integrated Nanotechnologies, Sandia National Laboratories, Albuquerque, USA

Publication Date:: 2020-11-24

Sponsoring Org.:: USDOE

OSTI Identifier:: 1724233

Resource Type:: Published Article

Journal Name:: RSC Advances

Additional Journal Information:: Journal Name: RSC Advances Journal Volume: 10 Journal Issue: 70; Journal ID: ISSN 2046-2069

Publisher:: Royal Society of Chemistry (RSC)

Country of Publication:: United Kingdom

Language:: English

Citation Formats


                    VanDelinder, Virginia, Sickafoose, Ian, Imam, Zachary I., Ko, Randy, and Bachand, George D. The effects of osmolytes on in vitro kinesin-microtubule motility assays.  United Kingdom: N. p., 2020. 
Web.  doi:10.1039/D0RA08148E.

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                    VanDelinder, Virginia, Sickafoose, Ian, Imam, Zachary I., Ko, Randy, & Bachand, George D. The effects of osmolytes on in vitro kinesin-microtubule motility assays.  United Kingdom.  https://doi.org/10.1039/D0RA08148E

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                    VanDelinder, Virginia, Sickafoose, Ian, Imam, Zachary I., Ko, Randy, and Bachand, George D. Tue .  
"The effects of osmolytes on in vitro kinesin-microtubule motility assays".  United Kingdom.  https://doi.org/10.1039/D0RA08148E.

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@article{osti_1724233,

  title        = {The effects of osmolytes on in vitro kinesin-microtubule motility assays},

  author       = {VanDelinder, Virginia and Sickafoose, Ian and Imam, Zachary I. and Ko, Randy and Bachand, George D.},

  abstractNote = {Kinesin-driven motility was shown to be adversely affected in a concentration dependent manner by the addition of osmolytes: glycerol, polyethylene glycol, and trimethylamine N -oxide.},

  doi          = {10.1039/D0RA08148E},

  journal      = {RSC Advances},

  number       = 70,

  volume       = 10,

  place        = {United Kingdom},

  year         = {2020},

  month        = {11}

}

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Works referenced in this record:

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Title: The effects of osmolytes on in vitro kinesin-microtubule motility assays

Abstract

Citation Formats

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In vitro depolymerization dynamics of brain endogenous microtubules
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Complete, rapid and reversible regulation of the motility of a nano-biomolecular machine using an osmolyte trimethylamine-N-oxide
journal, February 2020

Modulation of the Polymerization Kinetics of α/β-Tubulin by Osmolytes and Macromolecular Crowding
journal, January 2017

Effects of urea and trimethylamine-N-oxide on enzyme activity and stability
journal, November 1987

An unbiased detector of curvilinear structures
journal, January 1998

Transport of microtubules according to the number and spacing of kinesin motors on gold nano-pillars
journal, January 2019

Inhibition of Microtubule Depolymerization by Osmolytes
journal, April 2018

Synchrotron X-ray Diffraction Study of Microtubules Buckling and Bundling under Osmotic Stress: A Probe of Interprotofilament Interactions
journal, November 2004

Microtubules and Their Role in Cellular Stress in Cancer
journal, June 2014

Effects of osmotic force and torque on microtubule bundling and pattern formation
journal, October 2008

Crowding-induced organization of cytoskeletal elements. III. Spontaneous bundling and sorting of self-assembled filaments with different flexibilities
journal, December 1995

The stabilization of proteins by osmolytes
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journal, October 2006

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