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Title: Improving biomass and lipid yields of Desmodesmus armatus and Chlorella vulgaris through mutagenesis and high-throughput screening

Abstract

Advancement of microalgae for use as biofuel feedstock depends on bioprospecting novel strains and then applying classical strain improvement techniques to enhance desirable traits. Here we successfully demonstrate this approach using two novel strains of green algae, identified as Desmodesmus armatus and Chlorella vulgaris, to improve biomass and lipid yields. UV irradiation was utilized to generate random mutations and the resulting populations were analyzed via flow cytometry to quantify lipid fluorescence while enriching for high lipid variants using fluorescence activated cell sorting. Secondary screening was performed via in situ fluorescence staining to identify the single highest lipid producing mutants from each strain. This approach produced a Desmodesmus armatus mutant that exhibited both increased biomass productivity and higher lipid content, which doubled lipid yield from the culture. The resulting Chlorella vulgaris mutant exhibited an increase in growth only, resulting in a 33% increase in lipid yield.

Authors:
ORCiD logo [1]; ORCiD logo [1];  [1];  [1]; ORCiD logo [1];  [1]
  1. Univ. of California, San Diego, La Jolla, CA (United States)
Publication Date:
Research Org.:
Global Algae Innovations, San Diego, CA (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Contributing Org.:
Global Algae Innovations
OSTI Identifier:
1712419
Alternate Identifier(s):
OSTI ID: 1664575; OSTI ID: 1712417
Report Number(s):
DOE-GAI-7689-1
Journal ID: ISSN 0961-9534
Grant/Contract Number:  
EE0007689
Resource Type:
Accepted Manuscript
Journal Name:
Biomass and Bioenergy
Additional Journal Information:
Journal Volume: 142; Journal Issue: 105755; Journal ID: ISSN 0961-9534
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Microalgae; mutagenesis; strain improvement; FACS; Nile; red; biofuel

Citation Formats

Smalley, Tressa, Fields, Francis J., Berndt, Anthony J. E., Ostrand, Joseph T., Heredia, Vanessa, and Mayfield, Stephen P.. Improving biomass and lipid yields of Desmodesmus armatus and Chlorella vulgaris through mutagenesis and high-throughput screening. United States: N. p., 2020. Web. https://doi.org/10.1016/j.biombioe.2020.105755.
Smalley, Tressa, Fields, Francis J., Berndt, Anthony J. E., Ostrand, Joseph T., Heredia, Vanessa, & Mayfield, Stephen P.. Improving biomass and lipid yields of Desmodesmus armatus and Chlorella vulgaris through mutagenesis and high-throughput screening. United States. https://doi.org/10.1016/j.biombioe.2020.105755
Smalley, Tressa, Fields, Francis J., Berndt, Anthony J. E., Ostrand, Joseph T., Heredia, Vanessa, and Mayfield, Stephen P.. Wed . "Improving biomass and lipid yields of Desmodesmus armatus and Chlorella vulgaris through mutagenesis and high-throughput screening". United States. https://doi.org/10.1016/j.biombioe.2020.105755. https://www.osti.gov/servlets/purl/1712419.
@article{osti_1712419,
title = {Improving biomass and lipid yields of Desmodesmus armatus and Chlorella vulgaris through mutagenesis and high-throughput screening},
author = {Smalley, Tressa and Fields, Francis J. and Berndt, Anthony J. E. and Ostrand, Joseph T. and Heredia, Vanessa and Mayfield, Stephen P.},
abstractNote = {Advancement of microalgae for use as biofuel feedstock depends on bioprospecting novel strains and then applying classical strain improvement techniques to enhance desirable traits. Here we successfully demonstrate this approach using two novel strains of green algae, identified as Desmodesmus armatus and Chlorella vulgaris, to improve biomass and lipid yields. UV irradiation was utilized to generate random mutations and the resulting populations were analyzed via flow cytometry to quantify lipid fluorescence while enriching for high lipid variants using fluorescence activated cell sorting. Secondary screening was performed via in situ fluorescence staining to identify the single highest lipid producing mutants from each strain. This approach produced a Desmodesmus armatus mutant that exhibited both increased biomass productivity and higher lipid content, which doubled lipid yield from the culture. The resulting Chlorella vulgaris mutant exhibited an increase in growth only, resulting in a 33% increase in lipid yield.},
doi = {10.1016/j.biombioe.2020.105755},
journal = {Biomass and Bioenergy},
number = 105755,
volume = 142,
place = {United States},
year = {2020},
month = {9}
}

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