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Title: The effect of switchgrass plant cell wall properties on its deconstruction by thermochemical pretreatments coupled with fungal enzymatic hydrolysis or Clostridium thermocellum consolidated bioprocessing

Abstract

A combination of thermochemical pretreatment and biological digestion technologies is usually required to overcome lignocellulosic recalcitrance and accomplish effective biomass deconstruction. In this study, we aimed to understand switchgrass breakdown by hydrothermal, dilute acid, dilute alkali, and co-solvent enhanced lignocellulosic fractionation (CELF) pretreatments followed by application of traditional fungal enzymatic hydrolysis (EH) and Clostridium thermocellum consolidated bioprocessing (CBP) to the resulting solids. Unpretreated and pretreated switchgrass and their EH and CBP residues were characterized by a suite of analytical techniques to understand structural changes that occurred during deconstruction. CELF pretreated solids showed the highest accessibility and digestibility by both EH and CBP followed by dilute alkali and then dilute acid/hydrothermal pretreated solids. Lignin removal from biomass had a more positive impact on substrate accessibility and digestibility than did xylan removal, while xyloglucan removal by pretreatment appeared essential for cellulose digestion by fungal enzymes. The extent of CBP digestion of cellulose and non-cellulosic glycans was larger than that by EH. Unlike dilute alkali pretreatment, cellulose crystallinity increased for acid-based pretreatments in the following order: hydrothermal, dilute acid, and CELF. Acid-based pretreatments also substantially reduced cellulose degree of polymerization. All thermochemical and biological digestion approaches increased syringyl to guaiacyl lignin (S/G) ratiomore » and reduced β-O-4 lignin interunit linkage and hydroxycinnamates content from levels in unpretreated switchgrass. The substantial increase in S/G ratio after hydrothermal and dilute alkali preatreatments suggested that high temperatures or alkali removed a large portion of G lignin from switchgrass.« less

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [4]; ORCiD logo [3];  [5];  [5]; ORCiD logo [1];  [1]; ORCiD logo [5]; ORCiD logo [2]; ORCiD logo [1]
  1. Univ. of California, Riverside, CA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); State Univ. of New York (SUNY), Syracuse, NY (United States)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Georgia, Athens, GA (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1706247
Alternate Identifier(s):
OSTI ID: 1690310
Grant/Contract Number:  
AC05-00OR22725; PS02-06ER64304; FWP ERKP752
Resource Type:
Accepted Manuscript
Journal Name:
Green Chemistry
Additional Journal Information:
Journal Volume: N/A; Journal Issue: N/A; Journal ID: ISSN 1463-9262
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; bioethanol; Clostridium thermocellum; consolidated bioprocessing; pretreatment; switchgrass; enzymatic hydrolysis; fungal enzymes; lignocellulosic biomass

Citation Formats

Kothari, Ninad, Bhagia, Samarthya, Pu, Yunqiao Joseph, Yoo, Chang Geun, Li, Mi, Venketachalam, Sivasankari, Pattathil, Sivakumar, Kumar, Rajeev, Cai, Charles M., Hahn, Michael G., Ragauskas, Arthur J., and Wyman, Charles E.. The effect of switchgrass plant cell wall properties on its deconstruction by thermochemical pretreatments coupled with fungal enzymatic hydrolysis or Clostridium thermocellum consolidated bioprocessing. United States: N. p., 2020. Web. https://doi.org/10.1039/d0gc02546a.
Kothari, Ninad, Bhagia, Samarthya, Pu, Yunqiao Joseph, Yoo, Chang Geun, Li, Mi, Venketachalam, Sivasankari, Pattathil, Sivakumar, Kumar, Rajeev, Cai, Charles M., Hahn, Michael G., Ragauskas, Arthur J., & Wyman, Charles E.. The effect of switchgrass plant cell wall properties on its deconstruction by thermochemical pretreatments coupled with fungal enzymatic hydrolysis or Clostridium thermocellum consolidated bioprocessing. United States. https://doi.org/10.1039/d0gc02546a
Kothari, Ninad, Bhagia, Samarthya, Pu, Yunqiao Joseph, Yoo, Chang Geun, Li, Mi, Venketachalam, Sivasankari, Pattathil, Sivakumar, Kumar, Rajeev, Cai, Charles M., Hahn, Michael G., Ragauskas, Arthur J., and Wyman, Charles E.. Wed . "The effect of switchgrass plant cell wall properties on its deconstruction by thermochemical pretreatments coupled with fungal enzymatic hydrolysis or Clostridium thermocellum consolidated bioprocessing". United States. https://doi.org/10.1039/d0gc02546a. https://www.osti.gov/servlets/purl/1706247.
@article{osti_1706247,
title = {The effect of switchgrass plant cell wall properties on its deconstruction by thermochemical pretreatments coupled with fungal enzymatic hydrolysis or Clostridium thermocellum consolidated bioprocessing},
author = {Kothari, Ninad and Bhagia, Samarthya and Pu, Yunqiao Joseph and Yoo, Chang Geun and Li, Mi and Venketachalam, Sivasankari and Pattathil, Sivakumar and Kumar, Rajeev and Cai, Charles M. and Hahn, Michael G. and Ragauskas, Arthur J. and Wyman, Charles E.},
abstractNote = {A combination of thermochemical pretreatment and biological digestion technologies is usually required to overcome lignocellulosic recalcitrance and accomplish effective biomass deconstruction. In this study, we aimed to understand switchgrass breakdown by hydrothermal, dilute acid, dilute alkali, and co-solvent enhanced lignocellulosic fractionation (CELF) pretreatments followed by application of traditional fungal enzymatic hydrolysis (EH) and Clostridium thermocellum consolidated bioprocessing (CBP) to the resulting solids. Unpretreated and pretreated switchgrass and their EH and CBP residues were characterized by a suite of analytical techniques to understand structural changes that occurred during deconstruction. CELF pretreated solids showed the highest accessibility and digestibility by both EH and CBP followed by dilute alkali and then dilute acid/hydrothermal pretreated solids. Lignin removal from biomass had a more positive impact on substrate accessibility and digestibility than did xylan removal, while xyloglucan removal by pretreatment appeared essential for cellulose digestion by fungal enzymes. The extent of CBP digestion of cellulose and non-cellulosic glycans was larger than that by EH. Unlike dilute alkali pretreatment, cellulose crystallinity increased for acid-based pretreatments in the following order: hydrothermal, dilute acid, and CELF. Acid-based pretreatments also substantially reduced cellulose degree of polymerization. All thermochemical and biological digestion approaches increased syringyl to guaiacyl lignin (S/G) ratio and reduced β-O-4 lignin interunit linkage and hydroxycinnamates content from levels in unpretreated switchgrass. The substantial increase in S/G ratio after hydrothermal and dilute alkali preatreatments suggested that high temperatures or alkali removed a large portion of G lignin from switchgrass.},
doi = {10.1039/d0gc02546a},
journal = {Green Chemistry},
number = N/A,
volume = N/A,
place = {United States},
year = {2020},
month = {10}
}

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