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Title: Discovering the Molecular Determinants of Phaeobacter inhibens Susceptibility to Phaeobacter Phage MD18

Abstract

Bacteriophages are useful nonantibiotic therapeutics for bacterial infections as well as threats to industries utilizing bacterial agents. This study identified Phaeobacter virus MD18 , a phage antagonist of Phaeobacter inhibens , a bacterium with promising use as a probiotic for aquatic farming industries. Genomic analysis suggested that Phaeobacter phage MD18 has evolved to enhance its replication in P. inhibens by adopting favorable tRNA genes as well as through genomic sequence adaptation to resemble host codon usage. Lastly, a high-throughput analysis of P. inhibens transposon insertion mutants identified genes that modulate host susceptibility to phage MD18 and implicated the type IV pilus as the likely receptor recognized for adsorption. This study marks the first characterization of the relationship between P. inhibens and an environmentally sampled phage, which informs our understanding of natural threats to the bacterium and may promote the development of novel phage technologies for genetic manipulation of this host.

Authors:
ORCiD logo [1];  [2];  [3];  [4];  [5];  [6];
  1. Molecular Biology Interdepartmental Doctoral Program, University of California, Los Angeles, Los Angeles, California, USA
  2. Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA
  3. Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA
  4. Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
  5. Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA, Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA
  6. Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1698206
Grant/Contract Number:  
SC0016127
Resource Type:
Published Article
Journal Name:
mSphere
Additional Journal Information:
Journal Name: mSphere Journal Volume: 5 Journal Issue: 6; Journal ID: ISSN 2379-5042
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English

Citation Formats

Urtecho, Guillaume, Campbell, Danielle E., Hershey, David M., Hussain, Fatima A., Whitaker, Rachel J., O’Toole, George A., and Campbell, ed., Barbara J.. Discovering the Molecular Determinants of Phaeobacter inhibens Susceptibility to Phaeobacter Phage MD18. United States: N. p., 2020. Web. https://doi.org/10.1128/mSphere.00898-20.
Urtecho, Guillaume, Campbell, Danielle E., Hershey, David M., Hussain, Fatima A., Whitaker, Rachel J., O’Toole, George A., & Campbell, ed., Barbara J.. Discovering the Molecular Determinants of Phaeobacter inhibens Susceptibility to Phaeobacter Phage MD18. United States. https://doi.org/10.1128/mSphere.00898-20
Urtecho, Guillaume, Campbell, Danielle E., Hershey, David M., Hussain, Fatima A., Whitaker, Rachel J., O’Toole, George A., and Campbell, ed., Barbara J.. Wed . "Discovering the Molecular Determinants of Phaeobacter inhibens Susceptibility to Phaeobacter Phage MD18". United States. https://doi.org/10.1128/mSphere.00898-20.
@article{osti_1698206,
title = {Discovering the Molecular Determinants of Phaeobacter inhibens Susceptibility to Phaeobacter Phage MD18},
author = {Urtecho, Guillaume and Campbell, Danielle E. and Hershey, David M. and Hussain, Fatima A. and Whitaker, Rachel J. and O’Toole, George A. and Campbell, ed., Barbara J.},
abstractNote = {Bacteriophages are useful nonantibiotic therapeutics for bacterial infections as well as threats to industries utilizing bacterial agents. This study identified Phaeobacter virus MD18 , a phage antagonist of Phaeobacter inhibens , a bacterium with promising use as a probiotic for aquatic farming industries. Genomic analysis suggested that Phaeobacter phage MD18 has evolved to enhance its replication in P. inhibens by adopting favorable tRNA genes as well as through genomic sequence adaptation to resemble host codon usage. Lastly, a high-throughput analysis of P. inhibens transposon insertion mutants identified genes that modulate host susceptibility to phage MD18 and implicated the type IV pilus as the likely receptor recognized for adsorption. This study marks the first characterization of the relationship between P. inhibens and an environmentally sampled phage, which informs our understanding of natural threats to the bacterium and may promote the development of novel phage technologies for genetic manipulation of this host.},
doi = {10.1128/mSphere.00898-20},
journal = {mSphere},
number = 6,
volume = 5,
place = {United States},
year = {2020},
month = {12}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1128/mSphere.00898-20

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