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Title: Discovering the Molecular Determinants of Phaeobacter inhibens Susceptibility to Phaeobacter Phage MD18

Abstract

Bacteriophages have immense potential as antibiotic therapies and in genetic engineering. Understanding the mechanisms that bacteriophages implement to infect their hosts will allow researchers to manipulate these systems and adapt them to specific bacterial targets. In this study, we isolated a bacteriophage capable of infecting the marine alphaproteobacterium Phaeobacter inhibens and determined its mechanism of infection. Phaeobacter virus MD18, a novel species of bacteriophage isolated in Woods Hole, MA, exhibits potent lytic ability against P. inhibens and appears to be of the Siphoviridae morphotype. The genomic sequence of MD18 displayed significant similarity to another siphophage, the recently discovered Roseobacter phage DSS3P8, but genomic and phylogenetic analyses, assessing host range and a search of available metagenomes are all consistent with the conclusion that Phaeobacter phage MD18 is a novel lytic phage. We incubated MD18 with a library of barcoded P. inhibens transposon insertion mutants and identified 22 genes that appear to be required for phage predation of this host. Network analysis of these genes using genomic position, Gene Ontology (GO) term enrichment, and protein associations revealed that these genes are enriched for roles in assembly of a type IV pilus (T4P) and regulators of cellular morphology. Our results suggest that T4Pmore » serve as receptors for a novel marine virus that targets P. inhibens.« less

Authors:
ORCiD logo [1];  [2];  [3];  [4];  [5];  [6];
+ Show Author Affiliations
  1. Molecular Biology Interdepartmental Doctoral Program, University of California, Los Angeles, Los Angeles, California, USA
  2. Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA
  3. Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA
  4. Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
  5. Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA, Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA
  6. Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Hanover, New Hampshire, USA
Publication Date:
Research Org.:
Marine Biological Laboratory, Woods Hole, MA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); National Science Foundation (NSF); GGMI; Simons Foundation
OSTI Identifier:
1698206
Alternate Identifier(s):
OSTI ID: 1816479
Grant/Contract Number:  
SC0016127; MCB1822263; 5600373
Resource Type:
Published Article
Journal Name:
mSphere
Additional Journal Information:
Journal Name: mSphere Journal Volume: 5 Journal Issue: 6; Journal ID: ISSN 2379-5042
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bacteriophage; BarSeq; ecology; microbial interactions; phage genomics

Citation Formats

Urtecho, Guillaume, Campbell, Danielle E., Hershey, David M., Hussain, Fatima A., Whitaker, Rachel J., O’Toole, George A., and Campbell, ed., Barbara J. Discovering the Molecular Determinants of Phaeobacter inhibens Susceptibility to Phaeobacter Phage MD18. United States: N. p., 2020. Web. doi:10.1128/mSphere.00898-20.
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Urtecho, Guillaume, Campbell, Danielle E., Hershey, David M., Hussain, Fatima A., Whitaker, Rachel J., O’Toole, George A., & Campbell, ed., Barbara J. Discovering the Molecular Determinants of Phaeobacter inhibens Susceptibility to Phaeobacter Phage MD18. United States. https://doi.org/10.1128/mSphere.00898-20
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Urtecho, Guillaume, Campbell, Danielle E., Hershey, David M., Hussain, Fatima A., Whitaker, Rachel J., O’Toole, George A., and Campbell, ed., Barbara J. Wed . "Discovering the Molecular Determinants of Phaeobacter inhibens Susceptibility to Phaeobacter Phage MD18". United States. https://doi.org/10.1128/mSphere.00898-20.
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@article{osti_1698206,
title = {Discovering the Molecular Determinants of Phaeobacter inhibens Susceptibility to Phaeobacter Phage MD18},
author = {Urtecho, Guillaume and Campbell, Danielle E. and Hershey, David M. and Hussain, Fatima A. and Whitaker, Rachel J. and O’Toole, George A. and Campbell, ed., Barbara J.},
abstractNote = {Bacteriophages have immense potential as antibiotic therapies and in genetic engineering. Understanding the mechanisms that bacteriophages implement to infect their hosts will allow researchers to manipulate these systems and adapt them to specific bacterial targets. In this study, we isolated a bacteriophage capable of infecting the marine alphaproteobacterium Phaeobacter inhibens and determined its mechanism of infection. Phaeobacter virus MD18, a novel species of bacteriophage isolated in Woods Hole, MA, exhibits potent lytic ability against P. inhibens and appears to be of the Siphoviridae morphotype. The genomic sequence of MD18 displayed significant similarity to another siphophage, the recently discovered Roseobacter phage DSS3P8, but genomic and phylogenetic analyses, assessing host range and a search of available metagenomes are all consistent with the conclusion that Phaeobacter phage MD18 is a novel lytic phage. We incubated MD18 with a library of barcoded P. inhibens transposon insertion mutants and identified 22 genes that appear to be required for phage predation of this host. Network analysis of these genes using genomic position, Gene Ontology (GO) term enrichment, and protein associations revealed that these genes are enriched for roles in assembly of a type IV pilus (T4P) and regulators of cellular morphology. Our results suggest that T4P serve as receptors for a novel marine virus that targets P. inhibens.},
doi = {10.1128/mSphere.00898-20},
journal = {mSphere},
number = 6,
volume = 5,
place = {United States},
year = {Wed Dec 23 00:00:00 EST 2020},
month = {Wed Dec 23 00:00:00 EST 2020}
}
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https://doi.org/10.1128/mSphere.00898-20
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