Structure of the capsule and lipopolysaccharide O-antigen from the channel catfish pathogen, Aeromonas hydrophila
Abstract
A hypervirulent A. hydrophila (vAh) pathotype has been identified as the etiologic agent responsible for disease outbreaks in farmed carp species and channel catfish (Ictalurus punctatus) in China and the Southeastern United States, respectively. The possible route of infection has previously been unknown; however, virulence is believed to be multifactorial, involving the production/secretion of several virulence factors, including a high molecular weight group 4 capsular polysaccharide. Here we present chemical structural evidence of a novel capsule- and LPS-associated O-antigen found present in vAh isolated during these disease outbreaks. In this study, the chemical structure of the vAh O-antigen was determined by chemical analysis, Smith degradation, mass spectrometry, and 2D proton and carbon nuclear magnetic resonance (NMR) spectroscopy and found to be unique among described bacterial O-antigens. The O-antigen consists of hexasaccharide repeating units featuring a 4)-α-l-Fucp-(1–3)-β-d-GlcpNAc-(1–4)-α-l-Fucp-(1–4)-β-d-Glcp-(1- backbone, substituted with single residue side chains of α-d-Glcp and α-d-Quip3NAc linked to O-3 of the two fucose residues. The polysaccharide is partially O-acetylated on O-6 of the 4-substituted β-Glcp residue.
- Authors:
-
- Univ. of Georgia, Athens, GA (United States)
- Auburn Univ., AL (United States)
- Univ. of Michigan, Ann Arbor, MI (United States)
- Publication Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States); Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1802744
- Alternate Identifier(s):
- OSTI ID: 1691833; OSTI ID: 1964088
- Grant/Contract Number:
- SC0015662
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Carbohydrate Research
- Additional Journal Information:
- Journal Volume: 486; Journal ID: ISSN 0008-6215
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Aeromonas hydrophila; fish pathogen; O-antigen capsule; lipopolysaccharide; NMR; polysaccharide structure; Aeromonas hydrophila, fish pathogen, O-antigen capsule, lipopolysaccharide, NMR, polysaccharide structure
Citation Formats
Heiss, Christian, Wang, Zhirui, Thurlow, Charles M., Hossain, Mohammad J., Sun, Dawei, Liles, Mark R., Saper, Mark A., and Azadi, Parastoo. Structure of the capsule and lipopolysaccharide O-antigen from the channel catfish pathogen, Aeromonas hydrophila. United States: N. p., 2019.
Web. doi:10.1016/j.carres.2019.107858.
Heiss, Christian, Wang, Zhirui, Thurlow, Charles M., Hossain, Mohammad J., Sun, Dawei, Liles, Mark R., Saper, Mark A., & Azadi, Parastoo. Structure of the capsule and lipopolysaccharide O-antigen from the channel catfish pathogen, Aeromonas hydrophila. United States. https://doi.org/10.1016/j.carres.2019.107858
Heiss, Christian, Wang, Zhirui, Thurlow, Charles M., Hossain, Mohammad J., Sun, Dawei, Liles, Mark R., Saper, Mark A., and Azadi, Parastoo. Sat .
"Structure of the capsule and lipopolysaccharide O-antigen from the channel catfish pathogen, Aeromonas hydrophila". United States. https://doi.org/10.1016/j.carres.2019.107858. https://www.osti.gov/servlets/purl/1802744.
@article{osti_1802744,
title = {Structure of the capsule and lipopolysaccharide O-antigen from the channel catfish pathogen, Aeromonas hydrophila},
author = {Heiss, Christian and Wang, Zhirui and Thurlow, Charles M. and Hossain, Mohammad J. and Sun, Dawei and Liles, Mark R. and Saper, Mark A. and Azadi, Parastoo},
abstractNote = {A hypervirulent A. hydrophila (vAh) pathotype has been identified as the etiologic agent responsible for disease outbreaks in farmed carp species and channel catfish (Ictalurus punctatus) in China and the Southeastern United States, respectively. The possible route of infection has previously been unknown; however, virulence is believed to be multifactorial, involving the production/secretion of several virulence factors, including a high molecular weight group 4 capsular polysaccharide. Here we present chemical structural evidence of a novel capsule- and LPS-associated O-antigen found present in vAh isolated during these disease outbreaks. In this study, the chemical structure of the vAh O-antigen was determined by chemical analysis, Smith degradation, mass spectrometry, and 2D proton and carbon nuclear magnetic resonance (NMR) spectroscopy and found to be unique among described bacterial O-antigens. The O-antigen consists of hexasaccharide repeating units featuring a 4)-α-l-Fucp-(1–3)-β-d-GlcpNAc-(1–4)-α-l-Fucp-(1–4)-β-d-Glcp-(1- backbone, substituted with single residue side chains of α-d-Glcp and α-d-Quip3NAc linked to O-3 of the two fucose residues. The polysaccharide is partially O-acetylated on O-6 of the 4-substituted β-Glcp residue.},
doi = {10.1016/j.carres.2019.107858},
journal = {Carbohydrate Research},
number = ,
volume = 486,
place = {United States},
year = {Sat Oct 26 00:00:00 EDT 2019},
month = {Sat Oct 26 00:00:00 EDT 2019}
}
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