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Title: Near-Single-Cell Proteomics Profiling of the Proximal Tubular and Glomerulus of the Normal Human Kidney

Journal Article · · Frontiers in Medicine
 [1]; ORCiD logo [2];  [1];  [1]; ORCiD logo [2];  [2];  [3]; ORCiD logo [3];  [1];  [1];  [1];  [1];  [4];  [4]; ORCiD logo [2];  [1]
  1. Univ. of California, San Francisco, CA (United States)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
  4. Univ. of Michigan, Ann Arbor, MI (United States)
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Molecular assessments at the single-cell level can accelerate biological research by providing detailed assessments of cellular organization and tissue heterogeneity in both disease and health. The human kidney has complex multi-cellular states with varying functionality, much of which can now be completely harnessed with recent technological advances in tissue proteomics at a near single-cell level. We discuss the foundational steps in the first application of this mass spectrometry (MS) based proteomics method for analysis of sub-sections of the normal human kidney, as part of the Kidney Precision Medicine Project (KPMP). Using approximately 30-40 laser captured micro-dissected kidney cells, we identified more than 2500 human proteins, with specificity to the proximal tubular (PT; n=25 proteins) and glomerular (Glom; n=67 proteins) regions of the kidney and their unique metabolic functions. This pilot study provides the roadmap for application of our near-single-cell proteomics workflow for the analysis of other renal micro-compartments, on a larger scale, to unravel perturbations of renal sub-cellular function in the normal kidney as well as different etiologies of acute and chronic kidney disease.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Organization:
USDOE; National Institute of Diabetes, Digestive and Kidney Diseases (NIDDK); National Institutes of Health (NIH)
Contributing Organization:
Kidney Precision Medicine Project (KPMP) Consortium
Grant/Contract Number:
AC05-76RL01830
OSTI ID:
1677640
Report Number(s):
PNNL-SA-145047
Journal Information:
Frontiers in Medicine, Vol. 7; ISSN 2296-858X
Publisher:
Frontiers Media S.A.Copyright Statement
Country of Publication:
United States
Language:
English

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Figures / Tables (6)

Table 1(p. 22)table Table 1
Table 1 (Continued)(p. 23)table Table 1 (Continued)
Figure 1(p. 24)figure Figure 1
Figure 2(p. 26)figure Figure 2
Figure 3(p. 28)figure Figure 3
Figure 4(p. 29)figure Figure 4

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60 APPLIED LIFE SCIENCES
mass spectrometry
glomerulus
single cell analysis
proteomics
kidney