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Title: Clusters of Nanoscale Liposomes Modulate the Release of Encapsulated Species and Mimic the Compartmentalization Intrinsic in Cell Structures

Abstract

We report the ability to place a high concentration of liposomes in a confined volume as a multicompartment cluster that mimics biological cells and allows for the modulation of release of encapsulated species. The formation of these coated multicompartmental structures is achieved by first binding liposomes into clusters before encapsulating them within a two-dimensional metal–organic framework composed of tannic acid coordinated with a metal ion. The essential feature is a molecularly thin skin over a ssystem of clustered liposomes in a pouch. The structural features of these pouches are revealed by small-angle scattering and electron microscopy. Through cryogenic electron microscopy, clusters with intact liposomes are observed that appear to be encapsulated within a pouch. Small-angle X-ray scattering shows the emergence of a relatively weak Bragg peak at q = 0.125 Å–1, possibly indicating the attachment of the bilayers of adjacent liposomes. The metal–phenolic network (MPN) forms a nanosized conformal coating around liposome clusters, resulting in the reduced release rate of the encapsulated rhodamine B dye. We further show the possibility of communication between the adjacent nanocompartments in the cluster by demonstrating enhanced energy transfer using fluorescence resonance energy transfer (FRET) experiments where the lipophilic donor dye 3,3'-dioctadecyloxacarbocyanine perchlorate (DiO) incorporatedmore » within one liposomal compartment transfers energy upon excitation to the lipophilic acceptor dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) in a neighboring liposomal compartment due to their close proximity within the multicompartmental cluster. As a result, these observations have significance in adapting these multicompartmental structures that mimic biological cells for cascade reactions and as new depot drug delivery systems.« less

Authors:
ORCiD logo [1];  [1]; ORCiD logo [1]; ORCiD logo [1];  [1]; ORCiD logo [2]; ORCiD logo [1]
  1. Tulane University, New Orleans, LA (United States)
  2. National Institute of Standards and Technology, Gaithersburg, MD (United States)
Publication Date:
Research Org.:
Louisiana State Univ., Baton Rouge, LA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF)
OSTI Identifier:
1673153
Grant/Contract Number:  
SC0012432; 1805608
Resource Type:
Accepted Manuscript
Journal Name:
ACS Applied Nano Materials
Additional Journal Information:
Journal Volume: 2; Journal Issue: 11; Journal ID: ISSN 2574-0970
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; Coating materials; Vesicles; Encapsulation; Cluster chemistry; Fluorescence resonance energy transfer; Liposomes; Metal−phenolic network; Drug delivery; Cell mimics; FRET

Citation Formats

Mkam Tsengam, Igor Kevin, Omarova, Marzhana, Shepherd, Lauren, Sandoval, Nicholas, He, Jibao, Kelley, Elizabeth, and John, Vijay. Clusters of Nanoscale Liposomes Modulate the Release of Encapsulated Species and Mimic the Compartmentalization Intrinsic in Cell Structures. United States: N. p., 2019. Web. doi:10.1021/acsanm.9b01659.
Mkam Tsengam, Igor Kevin, Omarova, Marzhana, Shepherd, Lauren, Sandoval, Nicholas, He, Jibao, Kelley, Elizabeth, & John, Vijay. Clusters of Nanoscale Liposomes Modulate the Release of Encapsulated Species and Mimic the Compartmentalization Intrinsic in Cell Structures. United States. https://doi.org/10.1021/acsanm.9b01659
Mkam Tsengam, Igor Kevin, Omarova, Marzhana, Shepherd, Lauren, Sandoval, Nicholas, He, Jibao, Kelley, Elizabeth, and John, Vijay. Wed . "Clusters of Nanoscale Liposomes Modulate the Release of Encapsulated Species and Mimic the Compartmentalization Intrinsic in Cell Structures". United States. https://doi.org/10.1021/acsanm.9b01659. https://www.osti.gov/servlets/purl/1673153.
@article{osti_1673153,
title = {Clusters of Nanoscale Liposomes Modulate the Release of Encapsulated Species and Mimic the Compartmentalization Intrinsic in Cell Structures},
author = {Mkam Tsengam, Igor Kevin and Omarova, Marzhana and Shepherd, Lauren and Sandoval, Nicholas and He, Jibao and Kelley, Elizabeth and John, Vijay},
abstractNote = {We report the ability to place a high concentration of liposomes in a confined volume as a multicompartment cluster that mimics biological cells and allows for the modulation of release of encapsulated species. The formation of these coated multicompartmental structures is achieved by first binding liposomes into clusters before encapsulating them within a two-dimensional metal–organic framework composed of tannic acid coordinated with a metal ion. The essential feature is a molecularly thin skin over a ssystem of clustered liposomes in a pouch. The structural features of these pouches are revealed by small-angle scattering and electron microscopy. Through cryogenic electron microscopy, clusters with intact liposomes are observed that appear to be encapsulated within a pouch. Small-angle X-ray scattering shows the emergence of a relatively weak Bragg peak at q = 0.125 Å–1, possibly indicating the attachment of the bilayers of adjacent liposomes. The metal–phenolic network (MPN) forms a nanosized conformal coating around liposome clusters, resulting in the reduced release rate of the encapsulated rhodamine B dye. We further show the possibility of communication between the adjacent nanocompartments in the cluster by demonstrating enhanced energy transfer using fluorescence resonance energy transfer (FRET) experiments where the lipophilic donor dye 3,3'-dioctadecyloxacarbocyanine perchlorate (DiO) incorporated within one liposomal compartment transfers energy upon excitation to the lipophilic acceptor dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) in a neighboring liposomal compartment due to their close proximity within the multicompartmental cluster. As a result, these observations have significance in adapting these multicompartmental structures that mimic biological cells for cascade reactions and as new depot drug delivery systems.},
doi = {10.1021/acsanm.9b01659},
journal = {ACS Applied Nano Materials},
number = 11,
volume = 2,
place = {United States},
year = {2019},
month = {10}
}

Journal Article:
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Cited by: 6 works
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Figures / Tables:

Figure 1 Figure 1: (a) Structures of L-$α$-phosphatidylcholine and metal phenolic networks (MPN). Hydroxyl groups in tannic acid react with the Fe3+ ion to form MPN. (b) Schematic illustration of an MPN coated liposome pouch.

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Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.