OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate‐replete conditions
Abstract
Summary Plant Phosphate Transporter 1 (PHT1) proteins, probably the only influx transporters for phosphate (Pi) uptake, are partially degraded on sufficient Pi levels to prevent excessive Pi accumulation. Therefore, the basal/constitutive expression level of PHT1 genes is vital for maintaining Pi uptake under Pi‐replete conditions. Rice ( Oryza sativa ) OsPHT1;1 is a unique gene as it is highly expressed and not responsive to Pi, however the mechanism for maintaining its basal/constitutive expression remains unknown. Using biochemical and genetic approaches, we identified and functionally characterised the transcription factors maintaining the basal/constitutive expression of OsPHT1;1 . OsWRKY21 and OsWRKY108 interact within the nucleus and both bind to the W‐box in the OsPHT1;1 promoter. Overexpression of OsWRKY21 or OsWRKY108 led to increased Pi accumulation, resulting from elevated expression of OsPHT1;1 . By contrast, oswrky21 oswrky108 double mutants showed decreased Pi accumulation and OsPHT1;1 expression in a Pi‐dependent manner. Moreover, similar to ospht1;1 mutants, plants expressing the OsWRKY21–SRDX fusion protein (a chimeric dominant suppressor) were impaired in Pi accumulation in Pi‐replete roots, accompanied by downregulation of OsPHT1;1 expression. Our findings demonstrated that rice WRKY transcription factors function redundantly to promote Pi uptake by activating OsPHT1;1 expression under Pi‐replete conditions, and represent a novelmore »
- Authors:
-
[2];
[2]
- State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China
- State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China, MOA Key Laboratory of Plant Nutrition and Fertilisation in Lower‐Middle Reaches of the Yangtze River Nanjing 210095 China, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilisation Nanjing 210095 China
- State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China, MOA Key Laboratory of Plant Nutrition and Fertilisation in Lower‐Middle Reaches of the Yangtze River Nanjing 210095 China
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1671837
- Alternate Identifier(s):
- OSTI ID: 1781900
- Resource Type:
- Published Article
- Journal Name:
- New Phytologist
- Additional Journal Information:
- Journal Name: New Phytologist Journal Volume: 229 Journal Issue: 3; Journal ID: ISSN 0028-646X
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Zhang, Jun, Gu, Mian, Liang, Ruisuhua, Shi, Xinyu, Chen, Lingling, Hu, Xu, Wang, Shichao, Dai, Xiaoli, Qu, Hongye, Li, Huanhuan, and Xu, Guohua. OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate‐replete conditions. United Kingdom: N. p., 2020.
Web. doi:10.1111/nph.16931.
Zhang, Jun, Gu, Mian, Liang, Ruisuhua, Shi, Xinyu, Chen, Lingling, Hu, Xu, Wang, Shichao, Dai, Xiaoli, Qu, Hongye, Li, Huanhuan, & Xu, Guohua. OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate‐replete conditions. United Kingdom. https://doi.org/10.1111/nph.16931
Zhang, Jun, Gu, Mian, Liang, Ruisuhua, Shi, Xinyu, Chen, Lingling, Hu, Xu, Wang, Shichao, Dai, Xiaoli, Qu, Hongye, Li, Huanhuan, and Xu, Guohua. Sat .
"OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate‐replete conditions". United Kingdom. https://doi.org/10.1111/nph.16931.
@article{osti_1671837,
title = {OsWRKY21 and OsWRKY108 function redundantly to promote phosphate accumulation through maintaining the constitutive expression of OsPHT1;1 under phosphate‐replete conditions},
author = {Zhang, Jun and Gu, Mian and Liang, Ruisuhua and Shi, Xinyu and Chen, Lingling and Hu, Xu and Wang, Shichao and Dai, Xiaoli and Qu, Hongye and Li, Huanhuan and Xu, Guohua},
abstractNote = {Summary Plant Phosphate Transporter 1 (PHT1) proteins, probably the only influx transporters for phosphate (Pi) uptake, are partially degraded on sufficient Pi levels to prevent excessive Pi accumulation. Therefore, the basal/constitutive expression level of PHT1 genes is vital for maintaining Pi uptake under Pi‐replete conditions. Rice ( Oryza sativa ) OsPHT1;1 is a unique gene as it is highly expressed and not responsive to Pi, however the mechanism for maintaining its basal/constitutive expression remains unknown. Using biochemical and genetic approaches, we identified and functionally characterised the transcription factors maintaining the basal/constitutive expression of OsPHT1;1 . OsWRKY21 and OsWRKY108 interact within the nucleus and both bind to the W‐box in the OsPHT1;1 promoter. Overexpression of OsWRKY21 or OsWRKY108 led to increased Pi accumulation, resulting from elevated expression of OsPHT1;1 . By contrast, oswrky21 oswrky108 double mutants showed decreased Pi accumulation and OsPHT1;1 expression in a Pi‐dependent manner. Moreover, similar to ospht1;1 mutants, plants expressing the OsWRKY21–SRDX fusion protein (a chimeric dominant suppressor) were impaired in Pi accumulation in Pi‐replete roots, accompanied by downregulation of OsPHT1;1 expression. Our findings demonstrated that rice WRKY transcription factors function redundantly to promote Pi uptake by activating OsPHT1;1 expression under Pi‐replete conditions, and represent a novel pathway independent of the central Pi signalling system.},
doi = {10.1111/nph.16931},
journal = {New Phytologist},
number = 3,
volume = 229,
place = {United Kingdom},
year = {Sat Oct 10 00:00:00 EDT 2020},
month = {Sat Oct 10 00:00:00 EDT 2020}
}
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