pH-Induced reorientation of cytochrome c on silica nanoparticles

Meissner, Jens; Wu, Yao; Jestin, Jacques; Shelton, William A.; Findenegg, Gerhard H.; Bharti, Bhuvnesh

doi:10.1039/c8sm01909f

Title: pH-Induced reorientation of cytochrome c on silica nanoparticles

Journal Article · 15 November 2018 · Soft Matter

DOI: https://doi.org/10.1039/c8sm01909f · OSTI ID:1670686

Meissner, Jens ^[1]; Wu, Yao ^[2]; Jestin, Jacques ^[3];

^[2];

^[4];

^[2]

Technical University Berlin (Germany); Louisiana State University
Louisiana State University, Baton Rouge, LA (United States)
Laboratoire Léon Brillouin, Gif-sur-Yvette Cedex (France)
Technical University Berlin (Germany)

The orientation of cytochrome c molecules at the surface of silica nanoparticles was studied in a wide pH range by combining small-angle neutron scattering, adsorption measurements, and molecular dynamics simulations. Here, the results indicate a reorientation of the ellipsoidal protein from head-on to side-on as the pH is increased. This is attributed to changes in the surface charge distribution of both the protein and the nanoparticles.

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Research Organization:: Louisiana State University, Baton Rouge, LA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0012432

OSTI ID:: 1670686

Journal Information:: Soft Matter, Journal Name: Soft Matter Journal Issue: 3 Vol. 15; ISSN 1744-683X

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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