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Title: Nanoparticle surface charge influences translocation and leaf distribution in vascular plants with contrasting anatomy

Abstract

Root uptake and translocation of engineered nanoparticles (NPs) by plants are dependent on both plant species and NP physicochemical properties. To evaluate the influence of NP surface charge and differences in root structure and vasculature on cerium distribution and spatial distribution within plants, two monocotyledons (corn and rice) and two dicotyledons (tomato and lettuce) were exposed hydroponically to positively-charged, negatively-charged, and neutral ~4 nm CeO2 NPs. Here, leaves were analyzed using synchrotron-based X-ray fluorescence microscopy to provide lateral Ce spatial distribution. Surface charge mediated CeO2 NP interactions with roots for all plant species. Positively charged CeO2 NPs associated to the roots more than the negatively charged NPs due to electrostatic attraction/repulsion to the negatively charged root surfaces, with the highest association for the tomato, likely due to higher root surface area. The positive NPs remained primarily adhered to the roots untransformed, while the neutral and negative NPs were more efficiently translocated from the roots to shoots. This translocation efficiency was highest for the tomato and lettuce compared to corn and rice. Across all plant species, the positive and neutral treatments resulted in the formation of Ce clusters outside of the main vasculature in the mesophyll, while the negative treatment resultedmore » in Ce primarily in the main vasculature of the leaves. Comparing leaf vasculature, Ce was able to move much further outside of the main vasculature in the dicot plants than monocot plants, likely due to the larger airspace volume in dicot leaves compared to monocot leaves. These results provide valuable insight into the influence of plant structure and NP properties on metal transport and distribution of NPs in plants.« less

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [2];  [3]; ORCiD logo [4]; ORCiD logo [5]; ORCiD logo [1]
  1. Carnegie Mellon Univ., Pittsburgh, PA (United States)
  2. Brookhaven National Lab. (BNL), Upton, NY (United States)
  3. Brookhaven National Lab. (BNL), Upton, NY (United States); Univ. of Chicago, IL (United States)
  4. Univ. of Kentucky, Lexington, KY (United States)
  5. Univ. of California, Riverside, CA (United States)
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); USEPA; National Science Foundation (NSF)
OSTI Identifier:
1670663
Alternate Identifier(s):
OSTI ID: 1542518
Report Number(s):
BNL-219905-2020-JAAM
Journal ID: ISSN 2051-8153
Grant/Contract Number:  
SC0012704; FG02-92ER14244; CBET-1530563; EF-1266252; DGE-0966227
Resource Type:
Accepted Manuscript
Journal Name:
Environmental Science: Nano
Additional Journal Information:
Journal Volume: 6; Journal Issue: 8; Journal ID: ISSN 2051-8153
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Spielman-Sun, Eleanor, Avellan, Astrid, Bland, Garret D., Tappero, Ryan V., Acerbo, Alvin S., Unrine, Jason M., Giraldo, Juan Pablo, and Lowry, Gregory V. Nanoparticle surface charge influences translocation and leaf distribution in vascular plants with contrasting anatomy. United States: N. p., 2019. Web. https://doi.org/10.1039/c9en00626e.
Spielman-Sun, Eleanor, Avellan, Astrid, Bland, Garret D., Tappero, Ryan V., Acerbo, Alvin S., Unrine, Jason M., Giraldo, Juan Pablo, & Lowry, Gregory V. Nanoparticle surface charge influences translocation and leaf distribution in vascular plants with contrasting anatomy. United States. https://doi.org/10.1039/c9en00626e
Spielman-Sun, Eleanor, Avellan, Astrid, Bland, Garret D., Tappero, Ryan V., Acerbo, Alvin S., Unrine, Jason M., Giraldo, Juan Pablo, and Lowry, Gregory V. Mon . "Nanoparticle surface charge influences translocation and leaf distribution in vascular plants with contrasting anatomy". United States. https://doi.org/10.1039/c9en00626e. https://www.osti.gov/servlets/purl/1670663.
@article{osti_1670663,
title = {Nanoparticle surface charge influences translocation and leaf distribution in vascular plants with contrasting anatomy},
author = {Spielman-Sun, Eleanor and Avellan, Astrid and Bland, Garret D. and Tappero, Ryan V. and Acerbo, Alvin S. and Unrine, Jason M. and Giraldo, Juan Pablo and Lowry, Gregory V.},
abstractNote = {Root uptake and translocation of engineered nanoparticles (NPs) by plants are dependent on both plant species and NP physicochemical properties. To evaluate the influence of NP surface charge and differences in root structure and vasculature on cerium distribution and spatial distribution within plants, two monocotyledons (corn and rice) and two dicotyledons (tomato and lettuce) were exposed hydroponically to positively-charged, negatively-charged, and neutral ~4 nm CeO2 NPs. Here, leaves were analyzed using synchrotron-based X-ray fluorescence microscopy to provide lateral Ce spatial distribution. Surface charge mediated CeO2 NP interactions with roots for all plant species. Positively charged CeO2 NPs associated to the roots more than the negatively charged NPs due to electrostatic attraction/repulsion to the negatively charged root surfaces, with the highest association for the tomato, likely due to higher root surface area. The positive NPs remained primarily adhered to the roots untransformed, while the neutral and negative NPs were more efficiently translocated from the roots to shoots. This translocation efficiency was highest for the tomato and lettuce compared to corn and rice. Across all plant species, the positive and neutral treatments resulted in the formation of Ce clusters outside of the main vasculature in the mesophyll, while the negative treatment resulted in Ce primarily in the main vasculature of the leaves. Comparing leaf vasculature, Ce was able to move much further outside of the main vasculature in the dicot plants than monocot plants, likely due to the larger airspace volume in dicot leaves compared to monocot leaves. These results provide valuable insight into the influence of plant structure and NP properties on metal transport and distribution of NPs in plants.},
doi = {10.1039/c9en00626e},
journal = {Environmental Science: Nano},
number = 8,
volume = 6,
place = {United States},
year = {2019},
month = {7}
}

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Figures / Tables:

Fig. 1 Fig. 1: (Al Ce concentration (mg-Ce per kg of dried plant tissue) on/in dried roots (bottom) and shoots (top) and (Bl translocation efficiency (%, Tot Ceshoots/Tot Ceroots) of corn (yellow), rice (orange), tomato (light blue), and lettuce (dark blue) after 48 h of hydroponic exposure to 50 mg-Ce per Lasmore » CeO2(+), CeO2(0), or CeO2(-) NPs. Roots were rinsed for 30 s in Ce-free medium prior to lyophilization and analysis. The means are aver aged from four replicates. Error bars correspond to standard deviation. Significant differences [based on ANOVA and Tukey HSD post hoc tests (p < 0.05)] between plant species for the same NP treatment for either the roots or shoots are indicated by capital letters.« less

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