Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer

Wang, Yi-Ting; Shi, Tujin; Srivastava, Sudhir; Kagan, Jacob; Liu, Tao; Rodland, Karin D.

doi:10.3390/cancers12092335

Title: Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer

Journal Article · 18 August 2020 · Cancers (Basel)

DOI: https://doi.org/10.3390/cancers12092335 · OSTI ID:1668329

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^[1]; Srivastava, Sudhir ^[2]; Kagan, Jacob ^[2];

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Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
National Cancer Inst., Bethesda, MD (United States). Cancer Biomarkers Research Group
Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Oregon Health and Science Univ., Portland, OR (United States)

Extracellular vesicles (EVs) are released by nearly all cell types as part of normal cell physiology, transporting biological cargo, including nucleic acids and proteins, across the cell membrane. In pathological states such as cancer, EV-derived cargo may mirror the altered state of the cell of origin. Exosomes are the smaller, 50–150 nanometer-sized EVs released from fusion of multivesicular endosomes with the plasma membrane. Exosomes play important roles in cell-cell communication and participate in multiple cancer processes, including invasion and metastasis. Therefore, proteomic analysis of exosomes is a promising approach to discover potential cancer biomarkers, even though it is still at an early stage. Herein, we critically review the advances in exosome isolation methods and their compatibility with mass spectrometry (MS)-based proteomic analysis, as well as studies of exosomes in pathogenesis and progression of prostate and bladder cancer, two common urologic cancers whose incidence rates continue to rise annually. As urological tumors, both urine and blood samples are feasible for noninvasive or minimally invasive analysis. A better understanding of the biological cargo and functions of exosomes via high-throughput proteomics will help provide new insights into complex alterations in cancer and provide potential therapeutic targets and personalized treatment for patients.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1668329

Report Number(s):: PNNL-SA--154352

Journal Information:: Cancers (Basel), Journal Name: Cancers (Basel) Journal Issue: 9 Vol. 12; ISSN 2072-6694

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Handling and storage of human body fluids for analysis of extracellular vesicles Yuana, Yuana; Böing, Anita N.; Grootemaat, Anita E. Journal of Extracellular Vesicles, Vol. 4, Issue 1 https://doi.org/10.3402/jev.v4.29260	journal	January 2015
The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling Van Deun, Jan; Mestdagh, Pieter; Sormunen, Raija Journal of Extracellular Vesicles, Vol. 3, Issue 1 https://doi.org/10.3402/jev.v3.24858	journal	January 2014
Exosomes as Biomarker Enriched Microvesicles: Characterization of Exosomal Proteins Derived from a Panel of Prostate Cell Lines with Distinct AR Phenotypes Hosseini-Beheshti, Elham; Pham, Steven; Adomat, Hans Molecular & Cellular Proteomics, Vol. 11, Issue 10 https://doi.org/10.1074/mcp.M111.014845	journal	June 2012
Good things come in small packages: Overcoming challenges to harness extracellular vesicles for therapeutic delivery Ingato, Dominique; Lee, Jong Uk; Sim, Sang Jun Journal of Controlled Release, Vol. 241 https://doi.org/10.1016/j.jconrel.2016.09.016	journal	November 2016
Extracellular Vesicle (EV) Array: microarray capturing of exosomes and other extracellular vesicles for multiplexed phenotyping Jørgensen, Malene; Bæk, Rikke; Pedersen, Shona Journal of Extracellular Vesicles, Vol. 2, Issue 1 https://doi.org/10.3402/jev.v2i0.20920	journal	January 2013
Characterization of urinary extracellular vesicle proteins in muscle-invasive bladder cancer Silvers, Christopher R.; Miyamoto, Hiroshi; Messing, Edward M. Oncotarget, Vol. 8, Issue 53 https://doi.org/10.18632/oncotarget.20043	journal	August 2017
Methodology for isolation, identification and characterization of microvesicles in peripheral blood Jayachandran, Muthuvel; Miller, Virginia M.; Heit, John A. Journal of Immunological Methods, Vol. 375, Issue 1-2 https://doi.org/10.1016/j.jim.2011.10.012	journal	January 2012
Extracellular vesicle isolation and characterization: toward clinical application Xu, Rong; Greening, David W.; Zhu, Hong-Jian Journal of Clinical Investigation, Vol. 126, Issue 4 https://doi.org/10.1172/JCI81129	journal	April 2016
Single-step isolation of extracellular vesicles by size-exclusion chromatography Böing, Anita N.; van der Pol, Edwin; Grootemaat, Anita E. Journal of Extracellular Vesicles, Vol. 3, Issue 1 https://doi.org/10.3402/jev.v3.23430	journal	January 2014
Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy Jung, Min Kyo; Mun, Ji Young Journal of Visualized Experiments, Issue 131 https://doi.org/10.3791/56482	journal	January 2018
Vesiclepedia 2019: a compendium of RNA, proteins, lipids and metabolites in extracellular vesicles Pathan, Mohashin; Fonseka, Pamali; Chitti, Sai V. Nucleic Acids Research, Vol. 47, Issue D1 https://doi.org/10.1093/nar/gky1029	journal	November 2018
Targeted Quantification of Low ng/mL Level Proteins in Human Serum without Immunoaffinity Depletion Shi, Tujin; Sun, Xuefei; Gao, Yuqian Journal of Proteome Research, Vol. 12, Issue 7 https://doi.org/10.1021/pr400178v	journal	June 2013
Comparative and Targeted Proteomic Analyses of Urinary Microparticles from Bladder Cancer and Hernia Patients Chen, Chien-Lun; Lai, Yue-Fan; Tang, Petrus Journal of Proteome Research, Vol. 11, Issue 12 https://doi.org/10.1021/pr3008732	journal	October 2012

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