Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer

Wang, Yi-Ting; Shi, Tujin; Srivastava, Sudhir; Kagan, Jacob; Liu, Tao; Rodland, Karin D.

doi:10.3390/cancers12092335

Title: Proteomic Analysis of Exosomes for Discovery of Protein Biomarkers for Prostate and Bladder Cancer

Journal Article · 19 August 2020 · Cancers (Basel)

DOI: https://doi.org/10.3390/cancers12092335 · OSTI ID:1668329

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^[1]; Srivastava, Sudhir ^[2]; Kagan, Jacob ^[2];

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Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
National Cancer Inst., Bethesda, MD (United States). Cancer Biomarkers Research Group
Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Oregon Health and Science Univ., Portland, OR (United States)

Extracellular vesicles (EVs) are released by nearly all cell types as part of normal cell physiology, transporting biological cargo, including nucleic acids and proteins, across the cell membrane. In pathological states such as cancer, EV-derived cargo may mirror the altered state of the cell of origin. Exosomes are the smaller, 50–150 nanometer-sized EVs released from fusion of multivesicular endosomes with the plasma membrane. Exosomes play important roles in cell-cell communication and participate in multiple cancer processes, including invasion and metastasis. Therefore, proteomic analysis of exosomes is a promising approach to discover potential cancer biomarkers, even though it is still at an early stage. Herein, we critically review the advances in exosome isolation methods and their compatibility with mass spectrometry (MS)-based proteomic analysis, as well as studies of exosomes in pathogenesis and progression of prostate and bladder cancer, two common urologic cancers whose incidence rates continue to rise annually. As urological tumors, both urine and blood samples are feasible for noninvasive or minimally invasive analysis. A better understanding of the biological cargo and functions of exosomes via high-throughput proteomics will help provide new insights into complex alterations in cancer and provide potential therapeutic targets and personalized treatment for patients.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)

Grant/Contract Number:: AC05-76RL01830; U24CA210955; U01CA214116; R21CA223715; P41GM103493

OSTI ID:: 1668329

Report Number(s):: PNNL-SA-154352

Journal Information:: Cancers (Basel), Vol. 12, Issue 9; ISSN 2072-6694

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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