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Title: Genetic architecture of leaf morphological and physiological traits in a Populus deltoides ‘Danhong’ × P. simonii ‘Tongliao1’ pedigree revealed by quantitative trait locus analysis

Abstract

Understanding the genetic architecture of leaf morphological and physiological traits will help plant breeders develop high biomass poplar genotypes. Quantitative trait locus (QTL) studies combining next-generation sequencing techniques can advance our understanding of the genetic basis of complex traits. In this study, we measured 13 leaf morphological and physiological traits and identified quantitative trait loci (QTLs) in a Populus deltoides ‘Danhong’ × P. simonii ‘Tongliao1’ F1 population (500 progenies) using a high-density genetic map constructed by whole genome re-sequencing. This linkage map consisted of 5796 single nucleotide polymorphism (SNP) markers assigned to 19 linkage groups (LGs), spanning 2683.80 centimorgans (cM) of genetic length, with an average marker density of 0.46 cM. We identified 109 QTLs on 18 LGs for leaf morphological traits and 55 QTLs on 14 LGs for leaf physiological traits. One-hundred eight putative candidate genes were identified within the candidate genomic region. Co-expression network and gene ontology enrichment analyses suggested that these candidate genes were involved in the photosynthetic process. The differential expression patterns of the CYCLIN (Potri.015G112200) and RED CHLOROPHYLL REDUCTASE (Potri.007G043600) genes between two parents indicated their potential roles in leaf morphological and physiological traits. These findings decipher the genetic architecture of leaf morphological and physiologicalmore » traits in the P. deltoides ‘Danhong’ × P. simonii ‘Tongliao1’ pedigree and provide candidate genes for future poplar genetic improvement.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1]; ORCiD logo [2]; ORCiD logo [1]
  1. Chinese Academy of Forestry, Beijing (China)
  2. Chinese Academy of Forestry, Beijing (China) ; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE; National Key Research and Development Program of China; National Natural Science Foundation of China (NNSFC); National Key Program on Transgenic Research
OSTI Identifier:
1665983
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Tree Genetics & Genomes
Additional Journal Information:
Journal Volume: 16; Journal Issue: 3; Journal ID: ISSN 1614-2942
Country of Publication:
United States
Language:
English

Citation Formats

Sun, Pei, Jia, Huixia, Cheng, Xingqi, Zhang, Yahong, Li, Jianbo, Zhang, Lei, Lu, Mengzhu, Zhang, Jin, and Hu, Jianjun. Genetic architecture of leaf morphological and physiological traits in a Populus deltoides ‘Danhong’ × P. simonii ‘Tongliao1’ pedigree revealed by quantitative trait locus analysis. United States: N. p., 2020. Web. https://doi.org/10.1007/s11295-020-01438-y.
Sun, Pei, Jia, Huixia, Cheng, Xingqi, Zhang, Yahong, Li, Jianbo, Zhang, Lei, Lu, Mengzhu, Zhang, Jin, & Hu, Jianjun. Genetic architecture of leaf morphological and physiological traits in a Populus deltoides ‘Danhong’ × P. simonii ‘Tongliao1’ pedigree revealed by quantitative trait locus analysis. United States. https://doi.org/10.1007/s11295-020-01438-y
Sun, Pei, Jia, Huixia, Cheng, Xingqi, Zhang, Yahong, Li, Jianbo, Zhang, Lei, Lu, Mengzhu, Zhang, Jin, and Hu, Jianjun. Sun . "Genetic architecture of leaf morphological and physiological traits in a Populus deltoides ‘Danhong’ × P. simonii ‘Tongliao1’ pedigree revealed by quantitative trait locus analysis". United States. https://doi.org/10.1007/s11295-020-01438-y. https://www.osti.gov/servlets/purl/1665983.
@article{osti_1665983,
title = {Genetic architecture of leaf morphological and physiological traits in a Populus deltoides ‘Danhong’ × P. simonii ‘Tongliao1’ pedigree revealed by quantitative trait locus analysis},
author = {Sun, Pei and Jia, Huixia and Cheng, Xingqi and Zhang, Yahong and Li, Jianbo and Zhang, Lei and Lu, Mengzhu and Zhang, Jin and Hu, Jianjun},
abstractNote = {Understanding the genetic architecture of leaf morphological and physiological traits will help plant breeders develop high biomass poplar genotypes. Quantitative trait locus (QTL) studies combining next-generation sequencing techniques can advance our understanding of the genetic basis of complex traits. In this study, we measured 13 leaf morphological and physiological traits and identified quantitative trait loci (QTLs) in a Populus deltoides ‘Danhong’ × P. simonii ‘Tongliao1’ F1 population (500 progenies) using a high-density genetic map constructed by whole genome re-sequencing. This linkage map consisted of 5796 single nucleotide polymorphism (SNP) markers assigned to 19 linkage groups (LGs), spanning 2683.80 centimorgans (cM) of genetic length, with an average marker density of 0.46 cM. We identified 109 QTLs on 18 LGs for leaf morphological traits and 55 QTLs on 14 LGs for leaf physiological traits. One-hundred eight putative candidate genes were identified within the candidate genomic region. Co-expression network and gene ontology enrichment analyses suggested that these candidate genes were involved in the photosynthetic process. The differential expression patterns of the CYCLIN (Potri.015G112200) and RED CHLOROPHYLL REDUCTASE (Potri.007G043600) genes between two parents indicated their potential roles in leaf morphological and physiological traits. These findings decipher the genetic architecture of leaf morphological and physiological traits in the P. deltoides ‘Danhong’ × P. simonii ‘Tongliao1’ pedigree and provide candidate genes for future poplar genetic improvement.},
doi = {10.1007/s11295-020-01438-y},
journal = {Tree Genetics & Genomes},
number = 3,
volume = 16,
place = {United States},
year = {2020},
month = {5}
}

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