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Title: Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost

Abstract

Permafrost underlies a large portion of the land in the Northern Hemisphere. It is proposed to be an extreme habitat and home for cold-adaptive microbial communities. Upon thaw permafrost is predicted to exacerbate increasing global temperature trend, where awakening microbes decompose millennia old carbon stocks. Yet our knowledge on composition, functional potential and variance of permafrost microbiome remains limited. In this study, we conducted a deep comparative metagenomic analysis through a 2 m permafrost core from Svalbard, Norway to determine key permafrost microbiome in this climate sensitive island ecosystem. To do so, we developed comparative metagenomics methods on metagenomic-assembled genomes (MAG). We found that community composition in Svalbard soil horizons shifted markedly with depth: the dominant phylum switched from Acidobacteria and Proteobacteria in top soils (active layer) to Actinobacteria, Bacteroidetes, Chloroflexi and Proteobacteria in permafrost layers. Key metabolic potential propagated through permafrost depths revealed aerobic respiration and soil organic matter decomposition as key metabolic traits. We also found that Svalbard MAGs were enriched in genes involved in regulation of ammonium, sulfur and phosphate. Here, we provide a new perspective on how permafrost microbiome is shaped to acquire resources in competitive and limited resource conditions of deep Svalbard soils.

Authors:
 [1];  [1];  [2]; ORCiD logo [3]
  1. University of Bergen, Thormøhlensgt, Bergen, (Norway). Computational Biology Unit, Department of Informatics
  2. University of Bergen, Thormøhlensgt, Bergen, (Norway). Department of Biological Sciences; University Center in Svalbard, UNIS, Longyearbyen (Norway)
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Ecology Department, Earth and Environmental Sciences Area; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Genomics and Systems Biology, Biosciences Area
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1663231
Alternate Identifier(s):
OSTI ID: 1667371
Grant/Contract Number:  
AC02-05CH11231; 227062
Resource Type:
Accepted Manuscript
Journal Name:
FEMS Microbiology Ecology
Additional Journal Information:
Journal Volume: 96; Journal Issue: 5; Journal ID: ISSN 0168-6496
Publisher:
Federation of European Microbiology Societies
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Xue, Yaxin, Jonassen, Inge, Øvreås, Lise, and Taş, Neslihan. Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost. United States: N. p., 2020. Web. doi:10.1093/femsec/fiaa057.
Xue, Yaxin, Jonassen, Inge, Øvreås, Lise, & Taş, Neslihan. Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost. United States. doi:10.1093/femsec/fiaa057.
Xue, Yaxin, Jonassen, Inge, Øvreås, Lise, and Taş, Neslihan. Fri . "Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost". United States. doi:10.1093/femsec/fiaa057. https://www.osti.gov/servlets/purl/1663231.
@article{osti_1663231,
title = {Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost},
author = {Xue, Yaxin and Jonassen, Inge and Øvreås, Lise and Taş, Neslihan},
abstractNote = {Permafrost underlies a large portion of the land in the Northern Hemisphere. It is proposed to be an extreme habitat and home for cold-adaptive microbial communities. Upon thaw permafrost is predicted to exacerbate increasing global temperature trend, where awakening microbes decompose millennia old carbon stocks. Yet our knowledge on composition, functional potential and variance of permafrost microbiome remains limited. In this study, we conducted a deep comparative metagenomic analysis through a 2 m permafrost core from Svalbard, Norway to determine key permafrost microbiome in this climate sensitive island ecosystem. To do so, we developed comparative metagenomics methods on metagenomic-assembled genomes (MAG). We found that community composition in Svalbard soil horizons shifted markedly with depth: the dominant phylum switched from Acidobacteria and Proteobacteria in top soils (active layer) to Actinobacteria, Bacteroidetes, Chloroflexi and Proteobacteria in permafrost layers. Key metabolic potential propagated through permafrost depths revealed aerobic respiration and soil organic matter decomposition as key metabolic traits. We also found that Svalbard MAGs were enriched in genes involved in regulation of ammonium, sulfur and phosphate. Here, we provide a new perspective on how permafrost microbiome is shaped to acquire resources in competitive and limited resource conditions of deep Svalbard soils.},
doi = {10.1093/femsec/fiaa057},
journal = {FEMS Microbiology Ecology},
number = 5,
volume = 96,
place = {United States},
year = {2020},
month = {4}
}

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