Anaerobic respiration pathways and response to increased substrate availability of Arctic wetland soils
Abstract
The availability of labile carbon (C) compounds in Arctic wetland soils is expected to increase due to thawing permafrost and increased fermentation as a result of decomposition of organic matter with warming. How microbial communities respond to this change will affect the balance of CO2 and CH4 emitted during anaerobic organic matter decomposition, and ultimately the net radiative forcing of greenhouse gas emissions from these soils. While soil water content limits aerobic respiration, the factors controlling methanogenesis and anaerobic respiration are poorly defined in suboxic Arctic soils. In this study, we conducted incubation experiments on two tundra soils from field sites on the Seward Peninsula, Alaska, with contrasting pH and geochemistry to determine the pathways of anaerobic microbial respiration and changes with increasing substrate availability upon warming. In incubation of soils from the circumneutral Teller site, the ratio of CO2 to CH4 dropped from 10 to <2 after 60 days, indicating rapid depletion of alternative terminal electron acceptors (TEAs). Addition of acetate stimulated production of CO2 and CH4 in a nearly 1:1 ratio, consistent with methanogenesis, and the composition of the microbial community shifted to favor clades capable of utilizing the added acetate such as the Fe(III)-reducing Geobacter and themore »
- Authors:
-
[1];
[2];
[3];
[2];
[2];
[2]
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Hope College, Holland, MI (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Oakland Univ., Rochester, MI (United States)
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1661241
- Alternate Identifier(s):
- OSTI ID: 1658423
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Science: Processes & Impacts
- Additional Journal Information:
- Journal Volume: 22; Journal Issue: 9; Journal ID: ISSN 2050-7887
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES
Citation Formats
Philben, Michael J., Zhang, Lijie, Yang, Ziming, Taş, Neslihan, Wullschleger, Stan D., Graham, David E., and Gu, Baohua. Anaerobic respiration pathways and response to increased substrate availability of Arctic wetland soils. United States: N. p., 2020.
Web. doi:10.1039/d0em00124d.
Philben, Michael J., Zhang, Lijie, Yang, Ziming, Taş, Neslihan, Wullschleger, Stan D., Graham, David E., & Gu, Baohua. Anaerobic respiration pathways and response to increased substrate availability of Arctic wetland soils. United States. https://doi.org/10.1039/d0em00124d
Philben, Michael J., Zhang, Lijie, Yang, Ziming, Taş, Neslihan, Wullschleger, Stan D., Graham, David E., and Gu, Baohua. Fri .
"Anaerobic respiration pathways and response to increased substrate availability of Arctic wetland soils". United States. https://doi.org/10.1039/d0em00124d. https://www.osti.gov/servlets/purl/1661241.
@article{osti_1661241,
title = {Anaerobic respiration pathways and response to increased substrate availability of Arctic wetland soils},
author = {Philben, Michael J. and Zhang, Lijie and Yang, Ziming and Taş, Neslihan and Wullschleger, Stan D. and Graham, David E. and Gu, Baohua},
abstractNote = {The availability of labile carbon (C) compounds in Arctic wetland soils is expected to increase due to thawing permafrost and increased fermentation as a result of decomposition of organic matter with warming. How microbial communities respond to this change will affect the balance of CO2 and CH4 emitted during anaerobic organic matter decomposition, and ultimately the net radiative forcing of greenhouse gas emissions from these soils. While soil water content limits aerobic respiration, the factors controlling methanogenesis and anaerobic respiration are poorly defined in suboxic Arctic soils. In this study, we conducted incubation experiments on two tundra soils from field sites on the Seward Peninsula, Alaska, with contrasting pH and geochemistry to determine the pathways of anaerobic microbial respiration and changes with increasing substrate availability upon warming. In incubation of soils from the circumneutral Teller site, the ratio of CO2 to CH4 dropped from 10 to <2 after 60 days, indicating rapid depletion of alternative terminal electron acceptors (TEAs). Addition of acetate stimulated production of CO2 and CH4 in a nearly 1:1 ratio, consistent with methanogenesis, and the composition of the microbial community shifted to favor clades capable of utilizing the added acetate such as the Fe(III)-reducing Geobacter and the methanogenic archaea Methanosarcina. In contrast, both CO2 and CH4 production declined with acetate addition during incubation of soils from the more acidic Council site, and fermentative microorganisms increased in abundance despite the high availability of fermentation products. These results demonstrate that the degree to which increasing substrate availability stimulates greenhouse gas production in tundra wetlands will vary widely depending on soil pH and geochemistry.},
doi = {10.1039/d0em00124d},
journal = {Environmental Science: Processes & Impacts},
number = 9,
volume = 22,
place = {United States},
year = {Fri Aug 28 00:00:00 EDT 2020},
month = {Fri Aug 28 00:00:00 EDT 2020}
}
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