Cell-free gene expression: an expanded repertoire of applications
Abstract
Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-yielding (grams of protein per litre), cell-free gene expression systems from model bacteria, the development of cell-free platforms from non-model organisms and multiplexed strategies for rapidly assessing biological design. These advances provide exciting opportunities to profoundly transform synthetic biology by enabling new approaches to the model-driven design of synthetic gene networks, the fast and portable sensing of compounds, on-demand biomanufacturing, building cells from the bottom up, and next-generation educational kits.
- Authors:
-
[1];
[1];
[2]
- Northwestern Univ., Evanston, IL (United States). Chemistry of Life Processes Institute and Center for Synthetic Biology
- Northwestern Univ., Evanston, IL (United States); Northwestern Univ., Chicago, IL (United States). Center for Synthetic Biology, Robert H. Lurie Comprehensive Cancer Center, and Simpson Querrey Institute
- Publication Date:
- Research Org.:
- Northwestern Univ., Evanston, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1660454
- Alternate Identifier(s):
- OSTI ID: 2301593
- Grant/Contract Number:
- SC0018249; EE0008354; EE0008343
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Reviews. Genetics
- Additional Journal Information:
- Journal Volume: 21; Journal Issue: 3; Journal ID: ISSN 1471-0056
- Publisher:
- Springer Nature
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Silverman, Adam D., Karim, Ashty S., and Jewett, Michael C. Cell-free gene expression: an expanded repertoire of applications. United States: N. p., 2019.
Web. doi:10.1038/s41576-019-0186-3.
Silverman, Adam D., Karim, Ashty S., & Jewett, Michael C. Cell-free gene expression: an expanded repertoire of applications. United States. https://doi.org/10.1038/s41576-019-0186-3
Silverman, Adam D., Karim, Ashty S., and Jewett, Michael C. Thu .
"Cell-free gene expression: an expanded repertoire of applications". United States. https://doi.org/10.1038/s41576-019-0186-3. https://www.osti.gov/servlets/purl/1660454.
@article{osti_1660454,
title = {Cell-free gene expression: an expanded repertoire of applications},
author = {Silverman, Adam D. and Karim, Ashty S. and Jewett, Michael C.},
abstractNote = {Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-yielding (grams of protein per litre), cell-free gene expression systems from model bacteria, the development of cell-free platforms from non-model organisms and multiplexed strategies for rapidly assessing biological design. These advances provide exciting opportunities to profoundly transform synthetic biology by enabling new approaches to the model-driven design of synthetic gene networks, the fast and portable sensing of compounds, on-demand biomanufacturing, building cells from the bottom up, and next-generation educational kits.},
doi = {10.1038/s41576-019-0186-3},
journal = {Nature Reviews. Genetics},
number = 3,
volume = 21,
place = {United States},
year = {Thu Nov 28 00:00:00 EST 2019},
month = {Thu Nov 28 00:00:00 EST 2019}
}
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