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Title: Biomass Recalcitrance in Willow Under Two Biological Conversion Paradigms: Enzymatic Hydrolysis and Anaerobic Digestion

Abstract

Biomass recalcitrance, the inherent resistance of plants towards deconstruction, negatively affects the viability of biorefineries. This trait is not only dictated by the properties of the biomass but also by the conversion system used and its interactions with specific features of the biomass. Here, biomass recalcitrance to anaerobic digestion (AD) was assessed using a biomethanation potential (BMP) assay. Plant material (n = 94) was selected from a large population of natural Salix viminalis accessions, previously evaluated for biomass recalcitrance using hydrothermal pretreatment-enzymatic hydrolysis. Correlations between yields from the two biological conversion systems were evaluated, as well as the influence of biomass compositional features, analyzed by pyrolysis-molecular beam mass spectrometry (py-MBMS), and other biomass physical properties on conversion performance. BMP values averaged 198.0 Nml CH4/g biomass after 94 days, ranging from 28.6 to 245.9. S lignin and carbohydrate-derived spectral features were positively correlated with performance under both systems, whereas G lignin, p-coumaric acid, and ferulic acid-derived ions were negatively correlated with yields and rates. Most spectral features were more strongly correlated with enzymatic hydrolysis yields compared to methane production. For early-stage methane production and rate, recalcitrance factors were similar compared to enzymatic hydrolysis, with weaker correlations observed at later timepoints. Themore » results suggest that although variation in methane potential was considerably lower than enzymatic hydrolysis yields, a reduced recalcitrance under this system will still be of importance to improve early conversion rates. Spectral features of low methane-producing samples indicate the presence of inhibitory substances, warranting further study.« less

Authors:
 [1];  [2];  [2];  [2]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States). Biosciences Center, Center for Bioenergy Innovation
  2. Swedish Univ. of Agricultural Sciences, Uppsala (Sweden)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); Swedish Research Council (SRC)
OSTI Identifier:
1659772
Report Number(s):
NREL/JA-2700-74548
Journal ID: ISSN 1939-1234; MainId:19430;UUID:97c1f8f4-4db9-e911-9c24-ac162d87dfe5;MainAdminID:7908
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
BioEnergy Research
Additional Journal Information:
Journal Volume: 13; Journal ID: ISSN 1939-1234
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Salix viminalis; biomass recalcitrance; anaerobic digestion; biomethanation potential; enzymatic hydrolysis; analytical pyrolysis

Citation Formats

Ware, Anne, Ohlsson, Jonas A., Sandgren, Mats, and Schnurer, Anna. Biomass Recalcitrance in Willow Under Two Biological Conversion Paradigms: Enzymatic Hydrolysis and Anaerobic Digestion. United States: N. p., 2019. Web. https://doi.org/10.1007/s12155-019-10079-6.
Ware, Anne, Ohlsson, Jonas A., Sandgren, Mats, & Schnurer, Anna. Biomass Recalcitrance in Willow Under Two Biological Conversion Paradigms: Enzymatic Hydrolysis and Anaerobic Digestion. United States. https://doi.org/10.1007/s12155-019-10079-6
Ware, Anne, Ohlsson, Jonas A., Sandgren, Mats, and Schnurer, Anna. Sat . "Biomass Recalcitrance in Willow Under Two Biological Conversion Paradigms: Enzymatic Hydrolysis and Anaerobic Digestion". United States. https://doi.org/10.1007/s12155-019-10079-6. https://www.osti.gov/servlets/purl/1659772.
@article{osti_1659772,
title = {Biomass Recalcitrance in Willow Under Two Biological Conversion Paradigms: Enzymatic Hydrolysis and Anaerobic Digestion},
author = {Ware, Anne and Ohlsson, Jonas A. and Sandgren, Mats and Schnurer, Anna},
abstractNote = {Biomass recalcitrance, the inherent resistance of plants towards deconstruction, negatively affects the viability of biorefineries. This trait is not only dictated by the properties of the biomass but also by the conversion system used and its interactions with specific features of the biomass. Here, biomass recalcitrance to anaerobic digestion (AD) was assessed using a biomethanation potential (BMP) assay. Plant material (n = 94) was selected from a large population of natural Salix viminalis accessions, previously evaluated for biomass recalcitrance using hydrothermal pretreatment-enzymatic hydrolysis. Correlations between yields from the two biological conversion systems were evaluated, as well as the influence of biomass compositional features, analyzed by pyrolysis-molecular beam mass spectrometry (py-MBMS), and other biomass physical properties on conversion performance. BMP values averaged 198.0 Nml CH4/g biomass after 94 days, ranging from 28.6 to 245.9. S lignin and carbohydrate-derived spectral features were positively correlated with performance under both systems, whereas G lignin, p-coumaric acid, and ferulic acid-derived ions were negatively correlated with yields and rates. Most spectral features were more strongly correlated with enzymatic hydrolysis yields compared to methane production. For early-stage methane production and rate, recalcitrance factors were similar compared to enzymatic hydrolysis, with weaker correlations observed at later timepoints. The results suggest that although variation in methane potential was considerably lower than enzymatic hydrolysis yields, a reduced recalcitrance under this system will still be of importance to improve early conversion rates. Spectral features of low methane-producing samples indicate the presence of inhibitory substances, warranting further study.},
doi = {10.1007/s12155-019-10079-6},
journal = {BioEnergy Research},
number = ,
volume = 13,
place = {United States},
year = {2019},
month = {12}
}

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Works referenced in this record:

Lignin Transformation of One-Year-Old Plants During Anaerobic Digestion (AD)
journal, May 2019

  • Waliszewska, Hanna; Zborowska, Magdalena; Stachowiak-Wencek, Agata
  • Polymers, Vol. 11, Issue 5
  • DOI: 10.3390/polym11050835

Microbial biogas production from hydrolysis lignin: insight into lignin structural changes
journal, March 2018

  • Mulat, Daniel Girma; Dibdiakova, Janka; Horn, Svein Jarle
  • Biotechnology for Biofuels, Vol. 11, Issue 1
  • DOI: 10.1186/s13068-018-1054-7

The effect of aromatic structure on the inhibition of acetoclastic methanogenesis in granular sludge
journal, January 1991

  • Sierra-Alvarez, Reyes; Lettinga, Gatze
  • Applied Microbiology and Biotechnology, Vol. 34, Issue 4
  • DOI: 10.1007/BF00180586

Lessons from the cow: What the ruminant animal can teach us about consolidated bioprocessing of cellulosic biomass
journal, November 2009


New opportunities for agricultural digestate valorization: current situation and perspectives
journal, January 2015

  • Monlau, F.; Sambusiti, C.; Ficara, E.
  • Energy & Environmental Science, Vol. 8, Issue 9
  • DOI: 10.1039/C5EE01633A

Characteristics of Hot Water Extracts from the Bark of Cultivated Willow ( Salix sp.)
journal, February 2018


Toxic effects of phenolic pollutants on anaerobic benzoate-degrading granules
journal, January 1995

  • Fang, Herbert H. P.; Chen, T.; Chan, O. C.
  • Biotechnology Letters, Vol. 17, Issue 1
  • DOI: 10.1007/BF00134207

Biomass recalcitrance: a multi-scale, multi-factor, and conversion-specific property: Fig. 1.
journal, June 2015

  • McCann, Maureen C.; Carpita, Nicholas C.
  • Journal of Experimental Botany, Vol. 66, Issue 14
  • DOI: 10.1093/jxb/erv267

The potential of cellulases and cellulosomes for cellulosic waste management
journal, June 2007


Towards a standardization of biomethane potential tests
journal, September 2016

  • Holliger, Christof; Alves, Madalena; Andrade, Diana
  • Water Science and Technology, Vol. 74, Issue 11
  • DOI: 10.2166/wst.2016.336

Cellulosic ethanol: status and innovation
journal, June 2017


Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007

  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

The Path Forward for Biofuels and Biomaterials
journal, January 2006

  • Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
  • Science, Vol. 311, Issue 5760, p. 484-489
  • DOI: 10.1126/science.1114736

Bioenergy and biofuels: History, status, and perspective
journal, February 2015


Biogas-based polyhydroxyalkanoates production by Methylocystis hirsuta: A step further in anaerobic digestion biorefineries
journal, February 2018


Syringyl-Rich Lignin Renders Poplars More Resistant to Degradation by Wood Decay Fungi
journal, February 2013

  • Skyba, Oleksandr; Douglas, Carl J.; Mansfield, Shawn D.
  • Applied and Environmental Microbiology, Vol. 79, Issue 8
  • DOI: 10.1128/AEM.03182-12

Multiple levers for overcoming the recalcitrance of lignocellulosic biomass
journal, January 2019

  • Holwerda, Evert K.; Worthen, Robert S.; Kothari, Ninad
  • Biotechnology for Biofuels, Vol. 12, Issue 1
  • DOI: 10.1186/s13068-019-1353-7

Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems
journal, November 2017

  • Thomas, Vanessa A.; Kothari, Ninad; Bhagia, Samarthya
  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0975-x

The environmental biorefinery: state-of-the-art on the production of hydrogen and value-added biomolecules in mixed-culture fermentation
journal, January 2018

  • Moscoviz, Roman; Trably, Eric; Bernet, Nicolas
  • Green Chemistry, Vol. 20, Issue 14
  • DOI: 10.1039/C8GC00572A

Anaerobic digestion without biogas?
journal, September 2015

  • Kleerebezem, Robbert; Joosse, Bart; Rozendal, Rene
  • Reviews in Environmental Science and Bio/Technology, Vol. 14, Issue 4
  • DOI: 10.1007/s11157-015-9374-6

Factors Influencing the Variability of Antioxidative Phenolic Glycosides in Salix Species
journal, July 2010

  • Förster, Nadja; Ulrichs, Christian; Zander, Matthias
  • Journal of Agricultural and Food Chemistry, Vol. 58, Issue 14
  • DOI: 10.1021/jf100887v

Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass
journal, April 2011


Ferulic acid: a key component in grass lignocellulose recalcitrance to hydrolysis
journal, November 2014

  • de Oliveira, Dyoni Matias; Finger-Teixeira, Aline; Rodrigues Mota, Thatiane
  • Plant Biotechnology Journal, Vol. 13, Issue 9
  • DOI: 10.1111/pbi.12292

Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002

  • Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
  • Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
  • DOI: 10.1128/MMBR.66.3.506-577.2002

Genetic diversity, population structure and phenotypic variation in European Salix viminalis L. (Salicaceae)
journal, August 2014


Biogas production and saccharification of Salix pretreated at different steam explosion conditions
journal, September 2011


Lignocellulosic Materials Into Biohydrogen and Biomethane: Impact of Structural Features and Pretreatment
journal, January 2013

  • Monlau, Florian; Barakat, Abdellatif; Trably, Eric
  • Critical Reviews in Environmental Science and Technology, Vol. 43, Issue 3
  • DOI: 10.1080/10643389.2011.604258

Methane fermentation of woody biomass
journal, January 1991


Bioenergy: how much can we expect for 2050?
journal, September 2013


Effect of lignin-derived and furan compounds found in lignocellulosic hydrolysates on biomethane production
journal, January 2012


Cytotoxic constituents from the bark of Salix hulteni
journal, August 2008

  • Jeon, Seong Ho; Chun, Wanjoo; Choi, Yong Joon
  • Archives of Pharmacal Research, Vol. 31, Issue 8
  • DOI: 10.1007/s12272-001-1255-9

Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates
journal, June 2014


High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
journal, January 2015

  • Decker, Stephen R.; Sykes, Robert W.; Turner, Geoffrey B.
  • Journal of Visualized Experiments, Issue 103
  • DOI: 10.3791/53163

QTL Mapping of Wood FT-IR Chemotypes Shows Promise for Improving Biofuel Potential in Short Rotation Coppice Willow (Salix spp.)
journal, March 2018

  • Pawar, Prashant Mohan-Anupama; Schnürer, Anna; Mellerowicz, Ewa J.
  • BioEnergy Research, Vol. 11, Issue 2
  • DOI: 10.1007/s12155-018-9901-8

Methane production from solid-state anaerobic digestion of lignocellulosic biomass
journal, November 2012


Direct mass-spectrometric studies of the pyrolysis of carbonaceous fuels
journal, March 1986

  • Evans, Robert J.; Milne, Thomas A.; Soltys, Michael N.
  • Journal of Analytical and Applied Pyrolysis, Vol. 9, Issue 3
  • DOI: 10.1016/0165-2370(86)80012-2

Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population
journal, June 2019

  • Ohlsson, Jonas A.; Hallingbäck, Henrik R.; Jebrane, Mohamed
  • Biotechnology for Biofuels, Vol. 12, Issue 1
  • DOI: 10.1186/s13068-019-1479-7

Comparing methods for measuring the digestibility of miscanthus in bioethanol or biogas processing
journal, June 2016

  • Frydendal-Nielsen, Susanne; Jørgensen, Uffe; Hjorth, Maibritt
  • GCB Bioenergy, Vol. 9, Issue 1
  • DOI: 10.1111/gcbb.12377

Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure
journal, February 2016

  • Dumitrache, Alexandru; Akinosho, Hannah; Rodriguez, Miguel
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-016-0445-x

Inhibition of anaerobic digestion process: A review
journal, July 2008


Biogas: Developments and perspectives in Europe
journal, December 2018


Waste to bioproduct conversion with undefined mixed cultures: the carboxylate platform
journal, February 2011