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Title: A carotenoid-deficient mutant of the plant-associated microbe Pantoea sp. YR343 displays an altered membrane proteome

Abstract

Abstract Membrane organization plays an important role in signaling, transport, and defense. In eukaryotes, the stability, organization, and function of membrane proteins are influenced by certain lipids and sterols, such as cholesterol. Bacteria lack cholesterol, but carotenoids and hopanoids are predicted to play a similar role in modulating membrane properties. We have previously shown that the loss of carotenoids in the plant-associated bacteria Pantoea sp. YR343 results in changes to membrane biophysical properties and leads to physiological changes, including increased sensitivity to reactive oxygen species, reduced indole-3-acetic acid secretion, reduced biofilm and pellicle formation, and reduced plant colonization. Here, using whole cell and membrane proteomics, we show that the deletion of carotenoid production in Pantoea sp. YR343 results in altered membrane protein distribution and abundance. Moreover, we observe significant differences in the protein composition of detergent-resistant membrane fractions from wildtype and mutant cells, consistent with the prediction that carotenoids play a role in organizing membrane microdomains. These data provide new insights into the function of carotenoids in bacterial membrane organization and identify cellular functions that are affected by the loss of carotenoids.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1659527
Alternate Identifier(s):
OSTI ID: 1661233
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Published Article
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Name: Scientific Reports Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Microbiology; proteomic analysis

Citation Formats

Vijaya Kumar, Sushmitha, Abraham, Paul E., Hurst, Gregory B., Chourey, Karuna, Bible, Amber N., Hettich, Robert L., Doktycz, Mitchel J., and Morrell-Falvey, Jennifer L. A carotenoid-deficient mutant of the plant-associated microbe Pantoea sp. YR343 displays an altered membrane proteome. United Kingdom: N. p., 2020. Web. https://doi.org/10.1038/s41598-020-71672-w.
Vijaya Kumar, Sushmitha, Abraham, Paul E., Hurst, Gregory B., Chourey, Karuna, Bible, Amber N., Hettich, Robert L., Doktycz, Mitchel J., & Morrell-Falvey, Jennifer L. A carotenoid-deficient mutant of the plant-associated microbe Pantoea sp. YR343 displays an altered membrane proteome. United Kingdom. https://doi.org/10.1038/s41598-020-71672-w
Vijaya Kumar, Sushmitha, Abraham, Paul E., Hurst, Gregory B., Chourey, Karuna, Bible, Amber N., Hettich, Robert L., Doktycz, Mitchel J., and Morrell-Falvey, Jennifer L. Fri . "A carotenoid-deficient mutant of the plant-associated microbe Pantoea sp. YR343 displays an altered membrane proteome". United Kingdom. https://doi.org/10.1038/s41598-020-71672-w.
@article{osti_1659527,
title = {A carotenoid-deficient mutant of the plant-associated microbe Pantoea sp. YR343 displays an altered membrane proteome},
author = {Vijaya Kumar, Sushmitha and Abraham, Paul E. and Hurst, Gregory B. and Chourey, Karuna and Bible, Amber N. and Hettich, Robert L. and Doktycz, Mitchel J. and Morrell-Falvey, Jennifer L.},
abstractNote = {Abstract Membrane organization plays an important role in signaling, transport, and defense. In eukaryotes, the stability, organization, and function of membrane proteins are influenced by certain lipids and sterols, such as cholesterol. Bacteria lack cholesterol, but carotenoids and hopanoids are predicted to play a similar role in modulating membrane properties. We have previously shown that the loss of carotenoids in the plant-associated bacteria Pantoea sp. YR343 results in changes to membrane biophysical properties and leads to physiological changes, including increased sensitivity to reactive oxygen species, reduced indole-3-acetic acid secretion, reduced biofilm and pellicle formation, and reduced plant colonization. Here, using whole cell and membrane proteomics, we show that the deletion of carotenoid production in Pantoea sp. YR343 results in altered membrane protein distribution and abundance. Moreover, we observe significant differences in the protein composition of detergent-resistant membrane fractions from wildtype and mutant cells, consistent with the prediction that carotenoids play a role in organizing membrane microdomains. These data provide new insights into the function of carotenoids in bacterial membrane organization and identify cellular functions that are affected by the loss of carotenoids.},
doi = {10.1038/s41598-020-71672-w},
journal = {Scientific Reports},
number = 1,
volume = 10,
place = {United Kingdom},
year = {2020},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1038/s41598-020-71672-w

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