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Title: Geographic variation in the genetic basis of resistance to leaf rust between locally adapted ecotypes of the biofuel crop switchgrass (Panicum virgatum)

Abstract

Local adaptation is an important process in plant evolution, which can be impacted by differential pathogen pressures along environmental gradients. However, the degree to which pathogen resistance loci vary in effect across space and time is incompletely described. To understand how the genetic architecture of resistance varies across time and geographic space, we quantified rust (Puccinia spp.) severity in switchgrass (Panicum virgatum) plantings at eight locations across the central USA for 3 yr and conducted quantitative trait locus (QTL) mapping for rust progression. We mapped several variable QTLs, but two large-effect QTLs which we have named Prr1 and Prr2 were consistently associated with rust severity in multiple sites and years, particularly in northern sites. By contrast, there were numerous small-effect QTLs at southern sites, indicating a genotype-by-environment interaction in rust resistance loci. Interestingly, Prr1 and Prr2 had a strong epistatic interaction, which also varied in the strength and direction of effect across space. Our results suggest that abiotic factors covarying with latitude interact with the genetic loci underlying plant resistance to control rust infection severity. Furthermore, our results indicate that segregating genetic variation in epistatically interacting loci may play a key role in determining response to infection across geographic space.

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [4];  [5];  [6]; ORCiD logo [7]; ORCiD logo [8]; ORCiD logo [1]
  1. Michigan State Univ., East Lansing, MI (United States); USDOE Bioenergy Research Centers (BRC) (United States). Great Lakes Bioenergy Research Center (GLBRC)
  2. Univ. of Texas, Austin, TX (United States)
  3. Univ. of Missouri, Columbia, MO (United States)
  4. USDA‐Agricultural Research Service (ARS) Grassland, Temple, TX (United States)
  5. Univ. of Nebraska Lincoln NE (United States). USDA‐Agricultural Research Service (ARS) Wheat, Sorghum, and Forage Research Unit
  6. USDA‐Natural Resources Conservation Service (NRCS) Kika de la Garza Plant Materials Center, Kingsville, TX (United States)
  7. Texas A&M AgriLife Research and Extension Center Overton, TX (United States)
  8. Cornell Univ., Ithaca, NY (United States)
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States); USDOE Bioenergy Research Centers (BRC) (United States). Great Lakes Bioenergy Research Center (GLBRC); Univ. of Texas, Austin, TX (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)
OSTI Identifier:
1656820
Alternate Identifier(s):
OSTI ID: 1615701; OSTI ID: 1773851
Grant/Contract Number:  
SC0017883; SC0014156; IOS-0922457; IOS-1444533; SC0018409; FC02-07ER64494
Resource Type:
Accepted Manuscript
Journal Name:
New Phytologist
Additional Journal Information:
Journal Volume: 227; Journal Issue: 6; Journal ID: ISSN 0028-646X
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; GxE; local adaptation; pathogen resistance; Puccinia; QTL mapping; switchgrass (Panicum virgatum); pathogen QTL switchgrass

Citation Formats

VanWallendael, Acer, Bonnette, Jason, Juenger, Thomas E., Fritschi, Felix B., Fay, Philip A., Mitchell, Robert B., Lloyd‐Reilley, John, Rouquette, Jr., Francis M., Bergstrom, Gary C., and Lowry, David B. Geographic variation in the genetic basis of resistance to leaf rust between locally adapted ecotypes of the biofuel crop switchgrass (Panicum virgatum). United States: N. p., 2020. Web. https://doi.org/10.1111/nph.16555.
VanWallendael, Acer, Bonnette, Jason, Juenger, Thomas E., Fritschi, Felix B., Fay, Philip A., Mitchell, Robert B., Lloyd‐Reilley, John, Rouquette, Jr., Francis M., Bergstrom, Gary C., & Lowry, David B. Geographic variation in the genetic basis of resistance to leaf rust between locally adapted ecotypes of the biofuel crop switchgrass (Panicum virgatum). United States. https://doi.org/10.1111/nph.16555
VanWallendael, Acer, Bonnette, Jason, Juenger, Thomas E., Fritschi, Felix B., Fay, Philip A., Mitchell, Robert B., Lloyd‐Reilley, John, Rouquette, Jr., Francis M., Bergstrom, Gary C., and Lowry, David B. Mon . "Geographic variation in the genetic basis of resistance to leaf rust between locally adapted ecotypes of the biofuel crop switchgrass (Panicum virgatum)". United States. https://doi.org/10.1111/nph.16555. https://www.osti.gov/servlets/purl/1656820.
@article{osti_1656820,
title = {Geographic variation in the genetic basis of resistance to leaf rust between locally adapted ecotypes of the biofuel crop switchgrass (Panicum virgatum)},
author = {VanWallendael, Acer and Bonnette, Jason and Juenger, Thomas E. and Fritschi, Felix B. and Fay, Philip A. and Mitchell, Robert B. and Lloyd‐Reilley, John and Rouquette, Jr., Francis M. and Bergstrom, Gary C. and Lowry, David B.},
abstractNote = {Local adaptation is an important process in plant evolution, which can be impacted by differential pathogen pressures along environmental gradients. However, the degree to which pathogen resistance loci vary in effect across space and time is incompletely described. To understand how the genetic architecture of resistance varies across time and geographic space, we quantified rust (Puccinia spp.) severity in switchgrass (Panicum virgatum) plantings at eight locations across the central USA for 3 yr and conducted quantitative trait locus (QTL) mapping for rust progression. We mapped several variable QTLs, but two large-effect QTLs which we have named Prr1 and Prr2 were consistently associated with rust severity in multiple sites and years, particularly in northern sites. By contrast, there were numerous small-effect QTLs at southern sites, indicating a genotype-by-environment interaction in rust resistance loci. Interestingly, Prr1 and Prr2 had a strong epistatic interaction, which also varied in the strength and direction of effect across space. Our results suggest that abiotic factors covarying with latitude interact with the genetic loci underlying plant resistance to control rust infection severity. Furthermore, our results indicate that segregating genetic variation in epistatically interacting loci may play a key role in determining response to infection across geographic space.},
doi = {10.1111/nph.16555},
journal = {New Phytologist},
number = 6,
volume = 227,
place = {United States},
year = {2020},
month = {3}
}

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