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Title: Growth, total lipid, and omega-3 fatty acid production by Nannochloropsis spp. cultivated with raw plant substrate

Abstract

Improving productivity and lipid concentration in microalgae is important for the economic success of both biofuel and microalgae coproducts production. Nannochloropsis spp. are marine microalgae currently being grown at large-scale for the production of biofuel and lipid coproducts. In this work, we demonstrate improvements of growth and omega-3 production in Nannochloropsis gaditana CCMP526 and Nannochloropsis oceanica CCAP849/10 with plant substrate addition, a potentially economical option for increasing microalgal productivity. Furthermore, we examine growth in the presence of corn stover, switchgrass, sugarcane bagasse, and yard waste. By examining the microbial ecology of N. gaditana cultures with and without plant substrate and with and without antibiotics, we discovered a potential bacterial interaction in these cultures, but its presence is not necessary for algal growth improvements in the presence of plant substrate. Analysis of plant substrate morphology by scanning electron microscopy (SEM) after cultivation in media with and without N. gaditana shows a degradation of specific plant structural features and a colonization of the plant phloem by N. gaditana. An examination of N. gaditana in a Congo red plate assay indicates potential cellulolytic activity, but a preliminary examination of the potential cellulase transcripts does not reveal differential expression of a candidate in themore » presence of plant. This evidence demonstrates potential raw plant utilization by a marine microalga for increased productivity and provides a potentially economical option for increasing coproduct concentration at industrial scale without genetic engineering.« less

Authors:
 [1];  [1];  [2];  [2];  [1];  [3];  [1];  [4]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  2. U.S. Forest Service, Madison, WI (United States). Forest Products Lab.
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Conagen, Bedford, MA (United States)
  4. Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
OSTI Identifier:
1650143
Alternate Identifier(s):
OSTI ID: 1739994; OSTI ID: 1811141
Report Number(s):
SAND-2020-7729J; LA-UR-20-23298
Journal ID: ISSN 2211-9264; 687673
Grant/Contract Number:  
AC04-94AL85000; NL0035866; NL0029949; 89233218CNA000001
Resource Type:
Accepted Manuscript
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 51; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Schambach, Jenna Y., Finck, Anna M., Kitin, Peter, Hunt, Christopher G., Hanschen, Erik R., Vogler, Brian, Starkenburg, Shawn R., and Barry, Amanda N. Growth, total lipid, and omega-3 fatty acid production by Nannochloropsis spp. cultivated with raw plant substrate. United States: N. p., 2020. Web. https://doi.org/10.1016/j.algal.2020.102041.
Schambach, Jenna Y., Finck, Anna M., Kitin, Peter, Hunt, Christopher G., Hanschen, Erik R., Vogler, Brian, Starkenburg, Shawn R., & Barry, Amanda N. Growth, total lipid, and omega-3 fatty acid production by Nannochloropsis spp. cultivated with raw plant substrate. United States. https://doi.org/10.1016/j.algal.2020.102041
Schambach, Jenna Y., Finck, Anna M., Kitin, Peter, Hunt, Christopher G., Hanschen, Erik R., Vogler, Brian, Starkenburg, Shawn R., and Barry, Amanda N. Thu . "Growth, total lipid, and omega-3 fatty acid production by Nannochloropsis spp. cultivated with raw plant substrate". United States. https://doi.org/10.1016/j.algal.2020.102041.
@article{osti_1650143,
title = {Growth, total lipid, and omega-3 fatty acid production by Nannochloropsis spp. cultivated with raw plant substrate},
author = {Schambach, Jenna Y. and Finck, Anna M. and Kitin, Peter and Hunt, Christopher G. and Hanschen, Erik R. and Vogler, Brian and Starkenburg, Shawn R. and Barry, Amanda N.},
abstractNote = {Improving productivity and lipid concentration in microalgae is important for the economic success of both biofuel and microalgae coproducts production. Nannochloropsis spp. are marine microalgae currently being grown at large-scale for the production of biofuel and lipid coproducts. In this work, we demonstrate improvements of growth and omega-3 production in Nannochloropsis gaditana CCMP526 and Nannochloropsis oceanica CCAP849/10 with plant substrate addition, a potentially economical option for increasing microalgal productivity. Furthermore, we examine growth in the presence of corn stover, switchgrass, sugarcane bagasse, and yard waste. By examining the microbial ecology of N. gaditana cultures with and without plant substrate and with and without antibiotics, we discovered a potential bacterial interaction in these cultures, but its presence is not necessary for algal growth improvements in the presence of plant substrate. Analysis of plant substrate morphology by scanning electron microscopy (SEM) after cultivation in media with and without N. gaditana shows a degradation of specific plant structural features and a colonization of the plant phloem by N. gaditana. An examination of N. gaditana in a Congo red plate assay indicates potential cellulolytic activity, but a preliminary examination of the potential cellulase transcripts does not reveal differential expression of a candidate in the presence of plant. This evidence demonstrates potential raw plant utilization by a marine microalga for increased productivity and provides a potentially economical option for increasing coproduct concentration at industrial scale without genetic engineering.},
doi = {10.1016/j.algal.2020.102041},
journal = {Algal Research},
number = ,
volume = 51,
place = {United States},
year = {2020},
month = {10}
}

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This content will become publicly available on October 1, 2021
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