Maximizing enzymatic hydrolysis efficiency of bamboo with a mild ethanol-assistant alkaline peroxide pretreatment

Journal Article · 07 December 2019 · Bioresource Technology

DOI: https://doi.org/10.1016/j.biortech.2019.122568 · OSTI ID:1649143

Huang, Chen ^[1]; Fang, Guigan ^[2]; Yu, Longxiang ^[3]; Zhou, Yang ^[3]; Meng, Xianzhi ^[4]; Deng, Yongjun ^[5]; Shen, Kuizhong ^[5]; Ragauskas, Arthur J.  ^[6]

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1. Chinese Academy of Forestry, Nanjing (China). Inst. of Chemical Industry of Forest Products; Nanjing Forestry Univ. (China). Co-Innovation Center for Efficient Processing and Utilization of Forest Resources; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
2. Chinese Academy of Forestry, Nanjing (China). Inst. of Chemical Industry of Forest Products; Nanjing Forestry Univ. (China). Co-Innovation Center for Efficient Processing and Utilization of Forest Resources
3. Nanjing Forestry Univ. (China). Co-Innovation Center for Efficient Processing and Utilization of Forest Resources
4. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
5. Chinese Academy of Forestry, Nanjing (China). Inst. of Chemical Industry of Forest Products
6. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering and Dept. of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). UTK-ORNL Joint Institute for Biological Science, Biosciences Division

In this study, to overcome the delignification saturation point in traditional alkaline hydrogen peroxide pretreatment (AHP), a powerful modified AHP delignification methodology was established by introducing ethanol into the system. The pretreatment caused significant lignin removal of bamboo at elevated pretreatment temperature with the highest lignin removal reaching 80.0% at 100 °C, higher than that (74.9% lignin removal) in pretreatment without the ethanol assistance. In addition, a certain amount of carbohydrates was also solubilized during the process whose recovery was 83.3% (glucan) and 67.6% (hemicellulose), respectively. The pretreated solid exhibited excellent enzymatic digestibility, with hydrolysis yields of ~100% and 95.7% for glucan and xylan, respectively. Our studies further indicate that this delignification methodology is versatile for hardwood and herbaceous plants, but does not perform well on softwood.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE
• Grant/Contract Number: AC05-00OR22725; LHSXKQ8
• OSTI ID: 1649143
• Journal Information: Bioresource Technology, Vol. 299; ISSN 0960-8524
• Publisher: ElsevierCopyright Statement
• Country of Publication: United States
• Language: English