Maximizing enzymatic hydrolysis efficiency of bamboo with a mild ethanol-assistant alkaline peroxide pretreatment

Huang, Chen; Fang, Guigan; Yu, Longxiang; Zhou, Yang; Meng, Xianzhi; Deng, Yongjun; Shen, Kuizhong; Ragauskas, Arthur J.

doi:10.1016/j.biortech.2019.122568

Title: Maximizing enzymatic hydrolysis efficiency of bamboo with a mild ethanol-assistant alkaline peroxide pretreatment

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Abstract

In this study, to overcome the delignification saturation point in traditional alkaline hydrogen peroxide pretreatment (AHP), a powerful modified AHP delignification methodology was established by introducing ethanol into the system. The pretreatment caused significant lignin removal of bamboo at elevated pretreatment temperature with the highest lignin removal reaching 80.0% at 100 °C, higher than that (74.9% lignin removal) in pretreatment without the ethanol assistance. In addition, a certain amount of carbohydrates was also solubilized during the process whose recovery was 83.3% (glucan) and 67.6% (hemicellulose), respectively. The pretreated solid exhibited excellent enzymatic digestibility, with hydrolysis yields of ~100% and 95.7% for glucan and xylan, respectively. Our studies further indicate that this delignification methodology is versatile for hardwood and herbaceous plants, but does not perform well on softwood.

Authors:

Huang, Chen ^[1]; Fang, Guigan ^[2]; Yu, Longxiang ^[3]; Zhou, Yang ^[3]; Meng, Xianzhi ^[4]; Deng, Yongjun ^[5]; Shen, Kuizhong ^[5];

^[6]

Chinese Academy of Forestry, Nanjing (China). Inst. of Chemical Industry of Forest Products; Nanjing Forestry Univ. (China). Co-Innovation Center for Efficient Processing and Utilization of Forest Resources; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
Chinese Academy of Forestry, Nanjing (China). Inst. of Chemical Industry of Forest Products; Nanjing Forestry Univ. (China). Co-Innovation Center for Efficient Processing and Utilization of Forest Resources
Nanjing Forestry Univ. (China). Co-Innovation Center for Efficient Processing and Utilization of Forest Resources
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
Chinese Academy of Forestry, Nanjing (China). Inst. of Chemical Industry of Forest Products
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering and Dept. of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). UTK-ORNL Joint Institute for Biological Science, Biosciences Division

Publication Date:: 2019-12-07

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1649143

Grant/Contract Number:: AC05-00OR22725; LHSXKQ8

Resource Type:: Accepted Manuscript

Journal Name:: Bioresource Technology

Additional Journal Information:: Journal Volume: 299; Journal ID: ISSN 0960-8524

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; bamboo; low-temperature pretreatment; alkaline hydrogen peroxide; delignification; enzymatic hydrolysis

Citation Formats


                    Huang, Chen, Fang, Guigan, Yu, Longxiang, Zhou, Yang, Meng, Xianzhi, Deng, Yongjun, Shen, Kuizhong, and Ragauskas, Arthur J. Maximizing enzymatic hydrolysis efficiency of bamboo with a mild ethanol-assistant alkaline peroxide pretreatment.  United States: N. p., 2019. 
Web.  doi:10.1016/j.biortech.2019.122568.

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                    Huang, Chen, Fang, Guigan, Yu, Longxiang, Zhou, Yang, Meng, Xianzhi, Deng, Yongjun, Shen, Kuizhong, & Ragauskas, Arthur J. Maximizing enzymatic hydrolysis efficiency of bamboo with a mild ethanol-assistant alkaline peroxide pretreatment.  United States.  https://doi.org/10.1016/j.biortech.2019.122568

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                    Huang, Chen, Fang, Guigan, Yu, Longxiang, Zhou, Yang, Meng, Xianzhi, Deng, Yongjun, Shen, Kuizhong, and Ragauskas, Arthur J. Sat .  
"Maximizing enzymatic hydrolysis efficiency of bamboo with a mild ethanol-assistant alkaline peroxide pretreatment".  United States.  https://doi.org/10.1016/j.biortech.2019.122568.  https://www.osti.gov/servlets/purl/1649143.

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@article{osti_1649143,

  title        = {Maximizing enzymatic hydrolysis efficiency of bamboo with a mild ethanol-assistant alkaline peroxide pretreatment},

  author       = {Huang, Chen and Fang, Guigan and Yu, Longxiang and Zhou, Yang and Meng, Xianzhi and Deng, Yongjun and Shen, Kuizhong and Ragauskas, Arthur J.},

  abstractNote = {In this study, to overcome the delignification saturation point in traditional alkaline hydrogen peroxide pretreatment (AHP), a powerful modified AHP delignification methodology was established by introducing ethanol into the system. The pretreatment caused significant lignin removal of bamboo at elevated pretreatment temperature with the highest lignin removal reaching 80.0% at 100 °C, higher than that (74.9% lignin removal) in pretreatment without the ethanol assistance. In addition, a certain amount of carbohydrates was also solubilized during the process whose recovery was 83.3% (glucan) and 67.6% (hemicellulose), respectively. The pretreated solid exhibited excellent enzymatic digestibility, with hydrolysis yields of ~100% and 95.7% for glucan and xylan, respectively. Our studies further indicate that this delignification methodology is versatile for hardwood and herbaceous plants, but does not perform well on softwood.},

  doi          = {10.1016/j.biortech.2019.122568},

  journal      = {Bioresource Technology},

  number       = ,

  volume       = 299,

  place        = {United States},

  year         = {2019},

  month        = {12}

}

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