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Title: Enhancing isoprenoid synthesis in Yarrowia lipolytica by expressing the isopentenol utilization pathway and modulating intracellular hydrophobicity

Authors:
; ; ; ORCiD logo; ; ; ;
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1647108
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Metabolic Engineering
Additional Journal Information:
Journal Name: Metabolic Engineering Journal Volume: 61 Journal Issue: C; Journal ID: ISSN 1096-7176
Publisher:
Elsevier
Country of Publication:
Belgium
Language:
English

Citation Formats

Luo, Zhengshan, Liu, Nian, Lazar, Zbigniew, Chatzivasileiou, Alkiviadis, Ward, Valerie, Chen, Jian, Zhou, Jingwen, and Stephanopoulos, Gregory. Enhancing isoprenoid synthesis in Yarrowia lipolytica by expressing the isopentenol utilization pathway and modulating intracellular hydrophobicity. Belgium: N. p., 2020. Web. doi:10.1016/j.ymben.2020.07.010.
Luo, Zhengshan, Liu, Nian, Lazar, Zbigniew, Chatzivasileiou, Alkiviadis, Ward, Valerie, Chen, Jian, Zhou, Jingwen, & Stephanopoulos, Gregory. Enhancing isoprenoid synthesis in Yarrowia lipolytica by expressing the isopentenol utilization pathway and modulating intracellular hydrophobicity. Belgium. https://doi.org/10.1016/j.ymben.2020.07.010
Luo, Zhengshan, Liu, Nian, Lazar, Zbigniew, Chatzivasileiou, Alkiviadis, Ward, Valerie, Chen, Jian, Zhou, Jingwen, and Stephanopoulos, Gregory. Tue . "Enhancing isoprenoid synthesis in Yarrowia lipolytica by expressing the isopentenol utilization pathway and modulating intracellular hydrophobicity". Belgium. https://doi.org/10.1016/j.ymben.2020.07.010.
@article{osti_1647108,
title = {Enhancing isoprenoid synthesis in Yarrowia lipolytica by expressing the isopentenol utilization pathway and modulating intracellular hydrophobicity},
author = {Luo, Zhengshan and Liu, Nian and Lazar, Zbigniew and Chatzivasileiou, Alkiviadis and Ward, Valerie and Chen, Jian and Zhou, Jingwen and Stephanopoulos, Gregory},
abstractNote = {},
doi = {10.1016/j.ymben.2020.07.010},
journal = {Metabolic Engineering},
number = C,
volume = 61,
place = {Belgium},
year = {2020},
month = {9}
}

Works referenced in this record:

Isoprenoid biosynthesis in eukaryotic phototrophs: A spotlight on algae
journal, April 2012


Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals
journal, September 2016

  • Xu, Peng; Qiao, Kangjian; Ahn, Woo Suk
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 39
  • DOI: 10.1073/pnas.1607295113

Synthetic and systems biology for microbial production of commodity chemicals
journal, April 2016

  • Chubukov, Victor; Mukhopadhyay, Aindrila; Petzold, Christopher J.
  • npj Systems Biology and Applications, Vol. 2, Issue 1
  • DOI: 10.1038/npjsba.2016.9

Yarrowia lipolytica as a biotechnological chassis to produce usual and unusual fatty acids
journal, January 2016


Constructing Tailored Isoprenoid Products by Structure-Guided Modification of Geranylgeranyl Reductase
journal, July 2014


An Artificial Pathway for Isoprenoid Biosynthesis Decoupled from Native Hemiterpene Metabolism
journal, January 2019


Occurrence, biosynthesis and function of isoprenoid quinones
journal, September 2010


Introduction: Natural Product Synthesis
journal, August 2017


Regulation of lipid accumulation in oleaginous micro-organisms
journal, November 2002

  • Ratledge, C.
  • Biochemical Society Transactions, Vol. 30, Issue 6, p. 1047-1050
  • DOI: 10.1042/bst0301047

13C Metabolic Flux Analysis of acetate conversion to lipids by Yarrowia lipolytica
journal, November 2016


Current development in isoprenoid precursor biosynthesis and regulation
journal, August 2013

  • Chang, Wei-chen; Song, Heng; Liu, Hung-wen
  • Current Opinion in Chemical Biology, Vol. 17, Issue 4
  • DOI: 10.1016/j.cbpa.2013.06.020

Recent advances in bioengineering of the oleaginous yeast Yarrowia lipolytica
journal, January 2016

  • Shabbir Hussain, Murtaza; M. Rodriguez, Gabriel; Gao, Difeng
  • AIMS Bioengineering, Vol. 3, Issue 4
  • DOI: 10.3934/bioeng.2016.4.493

Extraction and Analysis of Terpenes/Terpenoids
journal, March 2016

  • Jiang, Zuodong; Kempinski, Chase; Chappell, Joe
  • Current Protocols in Plant Biology, Vol. 1, Issue 2
  • DOI: 10.1002/cppb.20024

Engineering a mevalonate pathway in Escherichia coli for production of terpenoids
journal, June 2003

  • Martin, Vincent J. J.; Pitera, Douglas J.; Withers, Sydnor T.
  • Nature Biotechnology, Vol. 21, Issue 7
  • DOI: 10.1038/nbt833

Improved squalene production through increasing lipid contents in Saccharomyces cerevisiae
journal, April 2018

  • Wei, Liu-Jing; Kwak, Suryang; Liu, Jing-Jing
  • Biotechnology and Bioengineering, Vol. 115, Issue 7
  • DOI: 10.1002/bit.26595

Cell free biosynthesis of isoprenoids from isopentenol
journal, September 2019

  • Ward, Valerie C. A.; Chatzivasileiou, Alkiviadis Orfefs; Stephanopoulos, Gregory
  • Biotechnology and Bioengineering, Vol. 116, Issue 12
  • DOI: 10.1002/bit.27146

Standardized Markerless Gene Integration for Pathway Engineering in Yarrowia lipolytica
journal, November 2016

  • Schwartz, Cory; Shabbir-Hussain, Murtaza; Frogue, Keith
  • ACS Synthetic Biology, Vol. 6, Issue 3
  • DOI: 10.1021/acssynbio.6b00285

Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli
journal, May 2005


Paclitaxel: biosynthesis, production and future prospects
journal, May 2014


Two-step pathway for isoprenoid synthesis
journal, December 2018

  • Chatzivasileiou, Alkiviadis Orfefs; Ward, Valerie; Edgar, Steven McBride
  • Proceedings of the National Academy of Sciences, Vol. 116, Issue 2
  • DOI: 10.1073/pnas.1812935116

The isoprenoid alcohol pathway, a synthetic route for isoprenoid biosynthesis
journal, June 2019

  • Clomburg, James M.; Qian, Shuai; Tan, Zaigao
  • Proceedings of the National Academy of Sciences, Vol. 116, Issue 26
  • DOI: 10.1073/pnas.1821004116

Production of isoprenoid pharmaceuticals by engineered microbes
journal, November 2006

  • Chang, Michelle C. Y.; Keasling, Jay D.
  • Nature Chemical Biology, Vol. 2, Issue 12
  • DOI: 10.1038/nchembio836

Isoprenoid lipid chains increase membrane resistance to pore formation
journal, October 2014

  • Panov, P. V.; Akimov, S. A.; Batishchev, O. V.
  • Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, Vol. 8, Issue 4
  • DOI: 10.1134/S1990747814050067

Metabolic engineering of Saccharomyces cerevisiae for production of butanol isomers
journal, June 2015


Lycopene: Progress in microbial production
journal, October 2016

  • Hernández-Almanza, Ayerim; Montañez, Julio; Martínez, Guillermo
  • Trends in Food Science & Technology, Vol. 56
  • DOI: 10.1016/j.tifs.2016.08.013

Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production
journal, January 2013


Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox metabolism
journal, January 2017

  • Qiao, Kangjian; Wasylenko, Thomas M.; Zhou, Kang
  • Nature Biotechnology, Vol. 35, Issue 2
  • DOI: 10.1038/nbt.3763

Exploring natural biodiversity to expand access to microbial terpene synthesis
journal, February 2019


Biotechnological applications of Yarrowia lipolytica: Past, present and future
journal, December 2015


The oxidative pentose phosphate pathway is the primary source of NADPH for lipid overproduction from glucose in Yarrowia lipolytica
journal, July 2015


The isoprenoid pathway and transcriptional response to its inhibitors in the yeast Saccharomyces cerevisiae
journal, February 2010


Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli
journal, September 2010


Recent advances in synthetic biology for engineering isoprenoid production in yeast
journal, October 2017

  • Vickers, Claudia E.; Williams, Thomas C.; Peng, Bingyin
  • Current Opinion in Chemical Biology, Vol. 40
  • DOI: 10.1016/j.cbpa.2017.05.017

Metabolic engineering of Escherichia coli for the production of isoprenoids
journal, March 2018

  • Ward, Valerie C. A.; Chatzivasileiou, Alkiviadis O.; Stephanopoulos, Gregory
  • FEMS Microbiology Letters, Vol. 365, Issue 10
  • DOI: 10.1093/femsle/fny079