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Title: Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus

Abstract

Histoplasma capsulatum is a dimorphic fungus that most frequently causes pneumonia, but can also disseminate and proliferate in diverse tissues. H. capsulatum has a complex secretion system that mediates the release of macromolecule-degrading enzymes and virulence factors. The formation and release of extracellular vesicles (EVs) are an important mechanism for non-conventional secretion in both ascomycetes and basidiomycetes. H. capsulatum EVs contain diverse proteins associated with virulence and are immunologically active. Despite the growing knowledge of EVs from H. capsulatum and other pathogenic fungi, the extent that changes in the environment impact the sorting of organic molecules in EVs has not been investigated. In our research, we cultivated H. capsulatum with distinct culture media to investigate the potential plasticity in EV loading in response to differences in nutrition. Our findings reveal that nutrition plays an important role in EV loading and formation, which may translate into differences in biological activities of these fungi in various fluids and tissues.

Authors:
ORCiD logo [1];  [1];  [2];  [3]; ORCiD logo [4];  [5];  [3];  [1]
  1. Albert Einstein College of Medicine, Bronx, NY (United States). Dept. of Medicine and Dept.of Microbiology and Immunology
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division; Albert Einstein College of Medicine, Bronx, NY (United States). Dept. of Medicine and Dept.of Microbiology and Immunology
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division
  4. Univ. Federal do Rio de Janeiro (UFRJ), Rio de Janeiro (Brazil). Inst. de Microbiologia Paulo de Góes
  5. Univ. Federal do Rio de Janeiro (UFRJ), Rio de Janeiro (Brazil). Inst. de Microbiologia Paulo de Góes; Fundação Oswaldo Cruz (Fiocruz), Curitiba (Brazil). Inst. Carlos Chagas
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE; National Institutes of Health (NIH); Brazilian Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação Oswaldo Cruz (Fiocruz)
OSTI Identifier:
1646823
Report Number(s):
PNNL-SA-151908
Journal ID: ISSN 1462-5814
Grant/Contract Number:  
AC05-76RL01830; R01AI052733; R21AI124797; 405520/2018-2; 440015/2018-9; 301304/2017-3; 311179/2017-7; 408711/2017-7; VPPCB-007-FIO-18-2- 57; VPPIS-001-FIO-18-66
Resource Type:
Accepted Manuscript
Journal Name:
Cellular Microbiology
Additional Journal Information:
Journal Volume: 22; Journal Issue: 9; Journal ID: ISSN 1462-5814
Country of Publication:
United States
Language:
English
Subject:
histoplasma capsulatum, histoplasmosis, dimorphic fungus, culture media, proteomics, lipidomics, metabolomics, BHI, Ham’s F12, RPMI, extracellular vesicles

Citation Formats

Cleare, Levi G., Zamith, Daniel, Heyman, Heino M., Couvillion, Sneha P., Nimrichter, Leonardo, Rodrigues, Marcio L., Nakayasu, Ernesto S., and Nosanchuk, Joshua D.. Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus. United States: N. p., 2020. Web. https://doi.org/10.1111/cmi.13217.
Cleare, Levi G., Zamith, Daniel, Heyman, Heino M., Couvillion, Sneha P., Nimrichter, Leonardo, Rodrigues, Marcio L., Nakayasu, Ernesto S., & Nosanchuk, Joshua D.. Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus. United States. https://doi.org/10.1111/cmi.13217
Cleare, Levi G., Zamith, Daniel, Heyman, Heino M., Couvillion, Sneha P., Nimrichter, Leonardo, Rodrigues, Marcio L., Nakayasu, Ernesto S., and Nosanchuk, Joshua D.. Mon . "Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus". United States. https://doi.org/10.1111/cmi.13217. https://www.osti.gov/servlets/purl/1646823.
@article{osti_1646823,
title = {Media matters! Alterations in the loading and release of Histoplasma capsulatum extracellular vesicles in response to different nutritional milieus},
author = {Cleare, Levi G. and Zamith, Daniel and Heyman, Heino M. and Couvillion, Sneha P. and Nimrichter, Leonardo and Rodrigues, Marcio L. and Nakayasu, Ernesto S. and Nosanchuk, Joshua D.},
abstractNote = {Histoplasma capsulatum is a dimorphic fungus that most frequently causes pneumonia, but can also disseminate and proliferate in diverse tissues. H. capsulatum has a complex secretion system that mediates the release of macromolecule-degrading enzymes and virulence factors. The formation and release of extracellular vesicles (EVs) are an important mechanism for non-conventional secretion in both ascomycetes and basidiomycetes. H. capsulatum EVs contain diverse proteins associated with virulence and are immunologically active. Despite the growing knowledge of EVs from H. capsulatum and other pathogenic fungi, the extent that changes in the environment impact the sorting of organic molecules in EVs has not been investigated. In our research, we cultivated H. capsulatum with distinct culture media to investigate the potential plasticity in EV loading in response to differences in nutrition. Our findings reveal that nutrition plays an important role in EV loading and formation, which may translate into differences in biological activities of these fungi in various fluids and tissues.},
doi = {10.1111/cmi.13217},
journal = {Cellular Microbiology},
number = 9,
volume = 22,
place = {United States},
year = {2020},
month = {6}
}

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