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Title: Agrobacterium tumefaciens : A Bacterium Primed for Synthetic Biology

Abstract

Agrobacterium tumefaciens is an important tool in plant biotechnology due to its natural ability to transfer DNA into the genomes of host plants. Genetic manipulations of A. tumefaciens have yielded considerable advances in increasing transformational efficiency in a number of plant species and cultivars. Moreover, there is overwhelming evidence that modulating the expression of various mediators of A. tumefaciens virulence can lead to more successful plant transformation; thus, the application of synthetic biology to enable targeted engineering of the bacterium may enable new opportunities for advancing plant biotechnology. In this review, we highlight engineering targets in both A. tumefaciens and plant hosts that could be exploited more effectively through precision genetic control to generate high-quality transformation events in a wider range of host plants. We then further discuss the current state of A. tumefaciens and plant engineering with regard to plant transformation and describe how future work may incorporate a rigorous synthetic biology approach to tailor strains of A. tumefaciens used in plant transformation.

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [1];  [3]; ORCiD logo [4]; ORCiD logo [2]; ORCiD logo [5]
  1. Joint BioEnergy Institute, Emeryville, CA, USA, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA, Department of Plant Biology, University of California-Davis, Davis, CA, USA
  2. Joint BioEnergy Institute, Emeryville, CA, USA, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA, Department of Plant and Microbial Biology, University of California-Berkeley, Berkeley, CA, USA
  3. Joint BioEnergy Institute, Emeryville, CA, USA, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
  4. Department of Plant Biology, University of California-Davis, Davis, CA, USA
  5. Joint BioEnergy Institute, Emeryville, CA, USA, Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA, Department of Plant Biology, University of California-Davis, Davis, CA, USA, Genome Center, University of California-Davis, Davis, CA, USA
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1639130
Alternate Identifier(s):
OSTI ID: 1777955
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Published Article
Journal Name:
BioDesign Research
Additional Journal Information:
Journal Name: BioDesign Research Journal Volume: 2020; Journal ID: ISSN 2693-1257
Publisher:
American Association for the Advancement of Science (AAAS)
Country of Publication:
India
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Thompson, Mitchell G., Moore, William M., Hummel, Niklas F. C., Pearson, Allison N., Barnum, Collin R., Scheller, Henrik V., and Shih, Patrick M. Agrobacterium tumefaciens : A Bacterium Primed for Synthetic Biology. India: N. p., 2020. Web. https://doi.org/10.34133/2020/8189219.
Thompson, Mitchell G., Moore, William M., Hummel, Niklas F. C., Pearson, Allison N., Barnum, Collin R., Scheller, Henrik V., & Shih, Patrick M. Agrobacterium tumefaciens : A Bacterium Primed for Synthetic Biology. India. https://doi.org/10.34133/2020/8189219
Thompson, Mitchell G., Moore, William M., Hummel, Niklas F. C., Pearson, Allison N., Barnum, Collin R., Scheller, Henrik V., and Shih, Patrick M. Tue . "Agrobacterium tumefaciens : A Bacterium Primed for Synthetic Biology". India. https://doi.org/10.34133/2020/8189219.
@article{osti_1639130,
title = {Agrobacterium tumefaciens : A Bacterium Primed for Synthetic Biology},
author = {Thompson, Mitchell G. and Moore, William M. and Hummel, Niklas F. C. and Pearson, Allison N. and Barnum, Collin R. and Scheller, Henrik V. and Shih, Patrick M.},
abstractNote = {Agrobacterium tumefaciens is an important tool in plant biotechnology due to its natural ability to transfer DNA into the genomes of host plants. Genetic manipulations of A. tumefaciens have yielded considerable advances in increasing transformational efficiency in a number of plant species and cultivars. Moreover, there is overwhelming evidence that modulating the expression of various mediators of A. tumefaciens virulence can lead to more successful plant transformation; thus, the application of synthetic biology to enable targeted engineering of the bacterium may enable new opportunities for advancing plant biotechnology. In this review, we highlight engineering targets in both A. tumefaciens and plant hosts that could be exploited more effectively through precision genetic control to generate high-quality transformation events in a wider range of host plants. We then further discuss the current state of A. tumefaciens and plant engineering with regard to plant transformation and describe how future work may incorporate a rigorous synthetic biology approach to tailor strains of A. tumefaciens used in plant transformation.},
doi = {10.34133/2020/8189219},
journal = {BioDesign Research},
number = ,
volume = 2020,
place = {India},
year = {2020},
month = {5}
}

Journal Article:
Free Publicly Available Full Text
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https://doi.org/10.34133/2020/8189219

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A GROBACTERIUM AND P LANT G ENES I NVOLVED IN T-DNA T RANSFER AND I NTEGRATION
journal, June 2000


Hfq Influences Multiple Transport Systems and Virulence in the Plant Pathogen Agrobacterium tumefaciens
journal, July 2012

  • Wilms, I.; Moller, P.; Stock, A. -M.
  • Journal of Bacteriology, Vol. 194, Issue 19
  • DOI: 10.1128/JB.00510-12

AGROBEST: an efficient Agrobacterium-mediated transient expression method for versatile gene function analyses in Arabidopsis seedlings
journal, January 2014


Agrobacterium-mediated horizontal gene transfer: Mechanism, biotechnological application, potential risk and forestalling strategy
journal, January 2019


A Plant Arabinogalactan-Like Glycoprotein Promotes a Novel Type of Polar Surface Attachment by Rhizobium leguminosarum
journal, February 2012

  • Xie, Fang; Williams, Alan; Edwards, Anne
  • Molecular Plant-Microbe Interactions, Vol. 25, Issue 2
  • DOI: 10.1094/MPMI-08-11-0211

Ethylene production in plants during transformation suppresses vir gene expression in Agrobacterium tumefaciens
journal, May 2008


Technical Focus: A guide to Agrobacterium binary Ti vectors
journal, October 2000


Arabidopsis RETICULON-LIKE3 (RTNLB3) and RTNLB8 Participate in Agrobacterium-Mediated Plant Transformation
journal, February 2018

  • Huang, Fan-Chen; Fu, Bi-Ju; Liu, Yin-Tzu
  • International Journal of Molecular Sciences, Vol. 19, Issue 2
  • DOI: 10.3390/ijms19020638

Inducible Expression of Agrobacterium Virulence Gene VirE2 for Stringent Regulation of T-DNA Transfer in Plant Transient Expression Systems
journal, November 2015

  • Denkovskienė, Erna; Paškevičius, Šarūnas; Werner, Stefan
  • Molecular Plant-Microbe Interactions®, Vol. 28, Issue 11
  • DOI: 10.1094/MPMI-05-15-0102-R

MAPK cascade signalling networks in plant defence
journal, August 2009

  • Pitzschke, Andrea; Schikora, Adam; Hirt, Heribert
  • Current Opinion in Plant Biology, Vol. 12, Issue 4
  • DOI: 10.1016/j.pbi.2009.06.008

The Agrobacterium Ti Plasmids
journal, December 2014


Cyclic diguanylic acid and cellulose synthesis in Agrobacterium tumefaciens.
journal, January 1989


Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions
journal, May 2007

  • Atmakuri, Krishnamohan; Cascales, Eric; Burton, Oliver T.
  • The EMBO Journal, Vol. 26, Issue 10
  • DOI: 10.1038/sj.emboj.7601696

virF, the host-range-determining virulence gene of Agrobacterium tumefaciens, affects T-DNA transfer to Zea mays.
journal, December 1991

  • Jarchow, E.; Grimsley, N. H.; Hohn, B.
  • Proceedings of the National Academy of Sciences, Vol. 88, Issue 23
  • DOI: 10.1073/pnas.88.23.10426

Molecular mechanism of RNA silencing suppression mediated by p19 protein of tombusviruses
journal, February 2004

  • Lakatos, Lóránt; Szittya, György; Silhavy, Dániel
  • The EMBO Journal, Vol. 23, Issue 4
  • DOI: 10.1038/sj.emboj.7600096

The VirE3 protein of Agrobacterium mimics a host cell function required for plant genetic transformation
journal, December 2004


T-DNA integration in plants results from polymerase-θ-mediated DNA repair
journal, October 2016


Agrobacterium infection and plant defense—transformation success hangs by a thread
journal, January 2013


Epigenetic silencing in transgenic plants
journal, September 2015

  • Rajeevkumar, Sarma; Anunanthini, Pushpanathan; Sathishkumar, Ramalingam
  • Frontiers in Plant Science, Vol. 6
  • DOI: 10.3389/fpls.2015.00693